Regulation of Gibberellin Biosynthesis in <i>Gibberella fujikuroi</i>

Candau, Reyes; Ávalos, Javier; Cerdá‐Olmedo, Enrique

doi:10.1104/pp.100.3.1184

Cited by 56 publications

(58 citation statements)

References 10 publications

(7 reference statements)

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“…As expected, the mRNA content for the three genes was low in the early stage of the cultures (2 days) and augmented in later stages (4 and 7 days) (Fig. 8B), when nitrogen is exhausted (47). However, in contrast to the GA3 amounts found in the cultures, mRNA contents in the wild type reached higher levels in the dark than under illumination, suggesting that CryD facilitates a more efficient translation of gib transcripts in the light, possibly linked to enhanced mRNA degradation.…”

Section: Resultsmentioning

confidence: 66%

Light-Dependent Functions of the Fusarium fujikuroi CryD DASH Cryptochrome in Development and Secondary Metabolism

Castrillo

García-Martínez

Ávalos

2013

Appl Environ Microbiol

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DASH (Drosophila, Arabidopsis, Synechocystis, human) cryptochromes (cry-DASHs) constitute a subgroup of the photolyase cryptochrome family with diverse light-sensing roles, found in most taxonomical groups. The genome of Fusarium fujikuroi, a phytopathogenic fungus with a rich secondary metabolism, contains a gene encoding a putative cry-DASH, named CryD. The expression of the cryD gene is induced by light in the wild type, but not in mutants of the "white collar" gene wcoA. Targeted ⌬cryD mutants show light-dependent phenotypic alterations, including changes in morphology and pigmentation, which disappear upon reintroduction of a wild-type cryD allele. In addition to microconidia, the colonies of the ⌬cryD mutants produced under illumination and nitrogen starvation large septated spores called macroconidia, absent in wild-type colonies. The ⌬cryD mutants accumulated similar amounts of carotenoids to the control strain under constant illumination, but produced much larger amounts of bikaverin under nitrogen starvation, indicating a repressing role for CryD in this biosynthetic pathway. Additionally, a moderate photoinduction of gibberellin production was exhibited by the wild type but not by the ⌬cryD mutants. The phenotypic alterations of the ⌬cryD mutants were only noticeable in the light, as expected from the low expression of cryD in the dark, but did not correlate with mRNA levels for structural genes of the bikaverin or gibberellin biosynthetic pathways, suggesting the participation of CryD in posttranscriptional regulatory mechanisms. This is the first report on the participation of a cry-DASH protein in the regulation of fungal secondary metabolism.

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Section: Resultsmentioning

confidence: 66%

Light-Dependent Functions of the Fusarium fujikuroi CryD DASH Cryptochrome in Development and Secondary Metabolism

Castrillo

García-Martínez

Ávalos

2013

Appl Environ Microbiol

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“…Furthermore, the GA 3 concentration in plants grown on low nitrate was higher than in plants grown on high nitrate both for both wild type and ga1-3 mutants (Figs 3a, b). Candau et al (1992) also reported that gibberellin biosynthesis occurred in lownitrogen medium but not in high-nitrogen medium, so we suggest that low nitrate may promote flowering by inducing the gibberellic acid pathway in wild type Arabidopsis. Yamauchi et al (2004) found the biosynthesis of GAs was not completely inhibited in ga1-3 mutants.…”

Section: Discussionmentioning

confidence: 99%

“…Marín et al (2011) reported that nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellins, and autonomous pathways. Gibberellin biosynthesis in Gibberella fujikuroi, however, occurred in low-nitrogen medium but not in high-nitrogen medium (Candau et al 1992). Exogenous GAs have been shown to promote the switch from vegetative growth to flowering in a variety of plants (Bernier 1988;Jacobsen & Olszewski 1993;Chandler & Dean 1994), but the interaction between GAs and nitrate on flowering has not been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Nitrate or NaCl regulates floral induction in Arabidopsis thaliana

Hou

Ren

et al. 2013

Biologia

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Flowering, the transition from the vegetative to reproductive phase in plants, is regulated by both endogenous and environmental signals. Exposure to an extended period of stress (such as low nitrate or NaCl) can also promote flowering in many species, but little is known about how these forms of stress regulate floral induction. In this study, we found that stress induced by low concentrations of nitrate or NaCl activated the biosynthesis of gibberellin (GA) as evidenced by increased expression of the GA biosynthetic enzyme GA1. Expression of CO and SOC1 were also enhanced, leading to an acceleration of flowering. The effects of nitrate and NaCl on the photoperiod pathway were distinct, however. Two genes related to the photoperiod pathway, CCA1 and LHY, were repressed only under low NaCl treatment, while expression was unaltered by nitrate. Therefore, we suggest that the biosynthesis of gibberellin (GA) may play an important role in integrating signals induced by exogenous stress to regulate flowering in Arabidopsis.

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“…High levels of both sugar and nitrogen have been found to be inhibitory to GAs production and responsiveness (Candau et al, 1992;Perata et al, 1997). It has been demonstrated that many magnolia genotypes prefer media containing a lower nitrogen concentration than that of MS medium (Biedermann, 1987;Kamenicka and Lanakova, 2000;Wojtania et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Morphological and Biochemical Responses to Gibberellic Acid in Magnolia × ‘Spectrum’ in Vitro

Wojtania

Skrzypek

Gabryszewska

2016

Acta Biologica Cracoviensia S. Botanica

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The total soluble sugar content and antioxidant enzyme activities were studied for the first time during axillary shoot formation in Magnolia × 'Spectrum' in vitro in response to BAP (0.3 mg l -1 ), different levels of gibberellic acid (GA 3 ; 0.0, 0.1, 0.5, 1.0 mg l -1 ), sucrose (20 and 30 g l -1 ) and nitrogen salts (KNO 3 /NH 4 NO 3 ; 100/100% and 75/50% relative to MS medium). Among various GA 3 and sucrose/nitrogen salts ratios, the most effective axillary multiplication (5.9 shoots/explant) and leaf formation (25.7 leaves per multiplied clumps) were obtained after addition of GA 3 at 0.1 mg l -1 to a BAP medium containing 20 g l -1 sucrose and reduced levels of nitrogen salts (75% KNO 3 and 50% NH 4 NO 3 ). The addition of GA 3 to the BAP medium enhanced shoot formation by 36% and leaf formation by 27%. The highest shoot formation capacity of M. × 'Spectrum' in vitro coincided with enhanced levels of soluble sugar and peroxidase (POD) activity. Increasing GA 3 concentration from 0.1 to 1.0 mg l -1 in the above medium resulted in inhibition of shoot and leaf formation and a decrease in the soluble sugar content. The influence of GA 3 on the activities of catalase (CAT) and POD depended on its concentration and the levels of sucrose and nitrogen salts in the medium. The highest increase in CAT and POD activities, that coincided with the enhanced shoot formation capacity of M. × 'Spectrum' in vitro, was observed after addition of GA 3 to the medium containing high levels of sucrose and nitrogen salts.

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Regulation of Gibberellin Biosynthesis in Gibberella fujikuroi

Cited by 56 publications

References 10 publications

Light-Dependent Functions of the Fusarium fujikuroi CryD DASH Cryptochrome in Development and Secondary Metabolism

Light-Dependent Functions of the Fusarium fujikuroi CryD DASH Cryptochrome in Development and Secondary Metabolism

Nitrate or NaCl regulates floral induction in Arabidopsis thaliana

Morphological and Biochemical Responses to Gibberellic Acid in Magnolia × ‘Spectrum’ in Vitro

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