Regulation of Formation of Monospecies and Binary Biofilms by Human Skin Microbiota Components, Staphylococcus epidermidis and Staphylococcus aureus, by Human Natriuretic Peptides

Ганнесен, А. В.; Lesouhaitier, Olivier; Нетрусов, А.И.; Плакунов, В. К.; Feuilloley, Marc

doi:10.1134/s0026261718050090

Cited by 16 publications

(34 citation statements)

References 31 publications

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“…We have recently shown that NUPs can affect significantly S. epidermidis and S. aureus biofilm formation activity (Gannesen et al, 2018b). Now we show the effect of ANP and CNP in the regulation of monospecies and mixed biofilms of C. acnes and S. aureus and explored for the first time the influence of oxygen concentrations and temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Monospecies biofilms of S. aureus MFP03 and C. acnes RT5 were obtained in 24-well plates with flat glass bottoms (Greiner Bio-One, Germany). Biofilms were grown according to Gannesen et al (2018a,b). Briefly, prepared cultures were washed twice with sterile PS and OD 580 was adjusted to 1.0.…”

Section: Methodsmentioning

confidence: 99%

“…The metabolic activity of cells in biofilms was determined as described by Gannesen et al (2018a,b), using the method of Plakunov et al (2016). Briefly, filters with biofilms were stained with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT).…”

Section: Methodsmentioning

confidence: 99%

“…The general principle of the determination of the effects of ANP and CNP were performed as described in Gannesen et al (2018a,b). Briefly, biofilms were grown on glass fiber filters Whatman GF/F in RCM in 12-well plates (Thermo Fisher Scientific, United States).…”

Section: Methodsmentioning

confidence: 99%

“…So, these two bacteria in this area are under permanent exposure to NUPs, which can potentially affect bacterial growth and biofilm formation as well as, through this, general skin status. Recently we have shown that ANPs and CNPs affect the growth of binary biofilms of S. aureus and S. epidermidis (Gannesen et al, 2018b). This work aims to investigate the impact of ANPs and CNPs on monospecies and binary biofilms of S. aureus and C. acnes .…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Monospecies and Mixed Biofilms Formation of Skin Staphylococcus aureus and Cutibacterium acnes by Human Natriuretic Peptides

et al. 2018

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Staphylococcus aureus and Cutibacterium acnes are common representatives of the human skin microbiome. However, when these bacteria are organized in biofilm, they could be involved in several skin disorders such as acne or psoriasis. They inhabit in hollows of hair follicles and skin glands, where they form biofilms. There, they are continuously exposed to human hormones, including human natriuretic peptides (NUPs). We first observed that the atrial natriuretic peptide (ANP) and the C-type natriuretic peptide (CNP) have a strong effect S. aureus and C. acnes biofilm formation on the skin. These effects are significantly dependent on the aero-anaerobic conditions and temperature. We also show that both ANP and CNP increased competitive advantages of C. acnes toward S. aureus in mixed biofilm. Because of their temperature-dependent effects, NUPs appear to act as a thermostat, allowing the skin to modulate bacterial development in normal and inflammatory conditions. This is an important step toward understanding how human neuroendocrine systems can regulate the cutaneous microbial community and should be important for applications in fundamental sciences, medicine, dermatology, and cosmetology.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of Monospecies and Mixed Biofilms Formation of Skin Staphylococcus aureus and Cutibacterium acnes by Human Natriuretic Peptides

et al. 2018

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Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Ганнесен

Schelkunov

Geras’kina

et al. 2021

Biofilm

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In this study, the effect of epinephrine on the biofilm formation of Micrococcus luteus C01 isolated from human skin was investigated in depth for the first time. This hormone has a complex effect on biofilms in various systems. In a system with polytetrafluoroethylene (PTFE) cubes, treatment with epinephrine at a physiological concentration of 4.9 × 10 −9 M increased the total amount of 72-h biofilm biomass stained with crystal violet and increased the metabolic activity of biofilms, but at higher and lower concentrations, the treatment had no significant effect. On glass fiber filters, treatment with the hormone decreased the number of colony forming units (CFUs) and changed the aggregation but did not affect the metabolic activity of biofilm cells. In glass bottom plates examined by confocal microscopy, epinephrine notably inhibited the growth of biofilms. RNA-seq analysis and RT–PCR demonstrated reproducible upregulation of genes encoding Fe–S cluster assembly factors and cyanide detoxification sulfurtransferase, whereas genes encoding the co-chaperone GroES, the LysE superfamily of lysine exporters, short-chain alcohol dehydrogenase and the potential c-di-GMP phosphotransferase were downregulated. Our results suggest that epinephrine may stimulate matrix synthesis in M. luteus biofilms, thereby increasing the activity of NAD(H) oxidoreductases. Potential c-di-GMP pathway proteins are essential in these processes.

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Structure of cell-wall glycopolymers of Micrococcus luteus C01

Zdorovenko

Ганнесен

Dmitrenok

et al. 2021

Carbohydrate Research

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Regulation of Formation of Monospecies and Binary Biofilms by Human Skin Microbiota Components, Staphylococcus epidermidis and Staphylococcus aureus, by Human Natriuretic Peptides

Cited by 16 publications

References 31 publications

Regulation of Monospecies and Mixed Biofilms Formation of Skin Staphylococcus aureus and Cutibacterium acnes by Human Natriuretic Peptides

Regulation of Monospecies and Mixed Biofilms Formation of Skin Staphylococcus aureus and Cutibacterium acnes by Human Natriuretic Peptides

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Structure of cell-wall glycopolymers of Micrococcus luteus C01

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