Regulation of expression of trehalose-6-phosphate synthase during cold shock in Arthrobacter strain A3

Chen, Ximing; Jiang, Ying; Li, Yuanting; Zhang, Haihong; Li, Jie; Chen, Xing; Zhao, Qi; Zhao, Jing; Si, Jing; Zhou, Lin; Zhang, Hua; Dyson, Paul; An, Lu

doi:10.1007/s00792-011-0380-5

Cited by 21 publications

(15 citation statements)

References 44 publications

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“…In E. coli , trehalose synthesis by enzymes OtsA and OtsB is induced by cold shock and is essential for cell viability [ 44 ]. In the psychrotolerant bacterium Arthrobacter strain A3, trehalose serves as a source of carbon, allowing cells to maintain normal metabolism at prolonged low temperatures [ 45 ]. Additional pathways for trehalose biosynthesis from maltose by the enzyme trehalose synthase ( treS ), and from maltodextrin by malto-oligosyl trehalose synthase ( treY ) and malto-oligosyl trehalose trehalohydrolase ( treZ ), were also identified in all Antarctic and temperate Arthrobacter genomes.…”

Section: Resultsmentioning

confidence: 99%

Genomic and phenotypic insights into the ecology of Arthrobacter from Antarctic soils

et al. 2015

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BackgroundMembers of the bacterial genus Arthrobacter are both readily cultured and commonly identified in Antarctic soil communities. Currently, relatively little is known about the physiological traits that allow these bacteria to survive in the harsh Antarctic soil environment. The aim of this study is to investigate if Antarctic strains of Arthrobacter owe their resilience to substantial genomic changes compared to Arthrobacter spp. isolated from temperate soil environments.ResultsQuantitative PCR-based analysis revealed that up to 4% of the soil bacterial communities were comprised of Arthrobacter spp. at four locations in the Ross Sea Region. Genome analysis of the seven Antarctic Arthrobacter isolates revealed several features that are commonly observed in psychrophilic/psychrotolerant bacteria. These include genes primarily associated with sigma factors, signal transduction pathways, the carotenoid biosynthesis pathway and genes induced by cold-shock, oxidative and osmotic stresses. However, these genes were also identified in genomes of seven temperate Arthrobacter spp., suggesting that these mechanisms are beneficial for growth and survival in a range of soil environments. Phenotypic characterisation revealed that Antarctic Arthrobacter isolates demonstrate significantly lower metabolic versatility and a narrower salinity tolerance range compared to temperate Arthrobacter species. Comparative analyses also revealed fewer protein-coding sequences and a significant decrease in genes associated with transcription and carbohydrate transport and metabolism in four of the seven Antarctic Arthrobacter isolates. Notwithstanding genome incompleteness, these differences together with the decreased metabolic versatility are indicative of genome content scaling.ConclusionsThe genomes of the seven Antarctic Arthrobacter isolates contained several features that may be beneficial for growth and survival in the Antarctic soil environment, although these features were not unique to the Antarctic isolates. These genome sequences allow further investigations into the expression of physiological traits that enable survival under extreme conditions and, more importantly, into the ability of these bacteria to respond to future perturbations including climate change and human impacts.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1220-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genomic and phenotypic insights into the ecology of Arthrobacter from Antarctic soils

et al. 2015

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“…Compared with the psychotrophic Arthrobacter strain A3, the Ar51 has the stronger ability to synthesis trehalose as the osmotic stress protector (Chen et al, 2011;Sun et al, 2016). In the genome of Ar51, it contains three pathways to synthesis trehalose: the OtsA/B (AU252_RS06465, AU252_RS06470, AU252_RS12615), TreY/TreZ (AU252_RS17210, AU252_RS17205) and TreS (AU252_RS10705, AU252_RS06525) pathways.…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence of a psychotrophic Pseudarthrobacter sulfonivorans strain Ar51 (CGMCC 4.7316), a novel crude oil and multi benzene compounds degradation strain

Zhang

Sun

Yang

et al. 2016

Journal of Biotechnology

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Pseudarthrobacter sulfonivorans strain Ar51, a psychotrophic bacterium isolated from the Tibet permafrost of China, can degrade crude oil and multi benzene compounds efficiently in low temperature. Here we report the complete genome sequence of this bacterium. The complete genome sequence of Pseudarthrobacter sulfonivorans strain Ar51, consisting of a cycle chromosome with a size of 5.04Mbp and a cycle plasmid with a size of 12.39kbp. The availability of this genome sequence allows us to investigate the genetic basis of crude oil degradation and adaptation to growth in a nutrient-poor permafrost environment.

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“…An otsA deletion mutant (Ar0002) has significantly reduced intracellular trehalose levels compared to the wild-type strain, Ar0001 ( S1A Fig ) [ 24 ]. We also observed that the mutant exhibits an apparent markedly slower growth rate in low osmolarity medium as determined by optical density (OD 600 ; Fig 1A ).…”

Section: Resultsmentioning

confidence: 99%

“…Arthrobacter sp. strain A3 (hereafter referred to as Arthrobacter A3), a psychrotrophic bacterium, was isolated from the alpine permafrost of the Tianshan Mountains in China [ 24 ]. It has an optimal growth temperature of 20 0 C, but can survive and grow at near-freezing temperatures as low as -4 0 C. Its stress tolerance is in part due to synthesis of trehalose catalyzed by OtsA/B [ 25 ] .…”

Section: Introductionmentioning

confidence: 99%

A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis

Chen

Fan

et al. 2017

PLoS Genet

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The dissacharide trehalose is an important intracellular osmoprotectant and the OtsA/B pathway is the principal pathway for trehalose biosynthesis in a wide range of bacterial species. Scaffolding proteins and other cytoskeletal elements play an essential role in morphogenetic processes in bacteria. Here we describe how OtsA, in addition to its role in trehalose biosynthesis, functions as an osmotic stress sensor to regulate cell morphology in Arthrobacter strain A3. In response to osmotic stress, this and other Arthrobacter species undergo a transition from bacillary to myceloid growth. An otsA null mutant exhibits constitutive myceloid growth. Osmotic stress leads to a depletion of trehalose-6-phosphate, the product of the OtsA enzyme, and experimental depletion of this metabolite also leads to constitutive myceloid growth independent of OtsA function. In vitro analyses indicate that OtsA can self-assemble into protein networks, promoted by trehalose-6-phosphate, a property that is not shared by the equivalent enzyme from E. coli, despite the latter’s enzymatic activity when expressed in Arthrobacter. This, and the localization of the protein in non-stressed cells at the mid-cell and poles, indicates that OtsA from Arthrobacter likely functions as a cytoskeletal element regulating cell morphology. Recruiting a biosynthetic enzyme for this morphogenetic function represents an intriguing adaptation in bacteria that can survive in extreme environments.

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Regulation of expression of trehalose-6-phosphate synthase during cold shock in Arthrobacter strain A3

Cited by 21 publications

References 44 publications

Genomic and phenotypic insights into the ecology of Arthrobacter from Antarctic soils

Genomic and phenotypic insights into the ecology of Arthrobacter from Antarctic soils

Complete genome sequence of a psychotrophic Pseudarthrobacter sulfonivorans strain Ar51 (CGMCC 4.7316), a novel crude oil and multi benzene compounds degradation strain

A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis

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