Regulation of expression by divalent cations of a light-inducible gene in Arthrobacter photogonimos

Divergent pathways for δ-aminolevulinic acid synthesis in two species of Arthrobacter

“…(ATCC 49663) and A. globi-,formis (ATCC 8010) were obtained from the American Type Culture Collection (Rockville, MD). As described previously [7], the characteristics of A. photogonimos are consistent with the genus designation Pimelobacter, which was introduced to refine classification of the heterogeneous coryneform bacteria [ 131. Cells were grown in the dark as described previously for A. photogonimos [7] for 5 h to obtain early exponential phase cells or 3 days for stationary phase cells.…”

“…As described previously [7], the characteristics of A. photogonimos are consistent with the genus designation Pimelobacter, which was introduced to refine classification of the heterogeneous coryneform bacteria [ 131. Cells were grown in the dark as described previously for A. photogonimos [7] for 5 h to obtain early exponential phase cells or 3 days for stationary phase cells. Cells were harvested by centrifugation at 5000 X g and suspended in a minimal medium containing 5 mM Na glutamate, 8 mM Na,HPO,, 0.8 mM KH?PO,, 1 mM (NH,J2S0,, and I mM MgCl?, pH 7.5, to an optical density at 450 nm of 2.0 (approximately 4 X IO' cells ml -' >.…”

“…In contrast, gabaculine does not inhibit ALA synthase [6] and the reactions from glutamate to succinyl-CoA result in loss of C-l of glutamate as CO,. In stubies with Arthrobacter photogonimos [7], we noticed that under some conditions this organism secreted large amounts of coproporphyrin III (Copro) into the medium. a feature shared by A. glob@mis…”

Molecular cloning of a light-inducible gene (lipA) encoding a novel pilin from Arthrobacter photogonimos

“…photogonimos (ATCC 49663) was characterized previously [1,4]. Cultures were treated overnight at 25³C with 1 WM neutral red and 15 W m 3P white £uorescent light and then LIP was washed from cells with 3 M NaCl containing 10 mM NaHCO Q (10 ml per ml packed cells).…”

“…Amino acid analysis, determination of N-terminal amino acid by dansylation, C-terminal sequence analysis by the kinetics of release of amino acids during digestion with carboxypeptidase Y [9], spectrophotometric assays for peptidase activity with amino acyl-p-nitroanilide substrates [11], and Western blot assays [4] were done as described previously. N-terminal amino acid sequencing was performed with a solid-phase sequencer.…”