Regulation of dendrite growth and maintenance by exocytosis

Peng, Yun; Lee, Jiae; Rowland, Kimberly; Wen, Yuhui; Hua, Hope; Carlson, Nicole E.; Lavania, Shweta; Parrish, Jay Z.; Kim, Michael D.

doi:10.1242/jcs.174771

Cited by 34 publications

(36 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S2 cells were cotransfected with Myc-Sec71 and different forms of (data not shown). Consistent with our observations, a previous study also reported Sec15 distribution in larval sensory neurons (Peng et al, 2015). Some Sec15-positive puncta were localized adjacent to or partially colocalized with the Arf1-positive Golgi apparatus (Fig.…”

Section: Sec71 Is An Arf1gef That Preferentially Interacts With Gdpbosupporting

confidence: 92%

Sec71 functions as a GEF for the small GTPase Arf1 to govern dendrite pruning of Drosophila sensory neurons

Wang¹,

Zhang²,

Shi³

et al. 2017

Journal of Cell Science

View full text Add to dashboard Cite

Pruning, whereby neurons eliminate their excess neurites, is central for the maturation of the nervous system. In Drosophila, sensory neurons, ddaCs, selectively prune their larval dendrites without affecting their axons during metamorphosis. However, it is unknown whether the secretory pathway plays a role in dendrite pruning. Here, we show that the small GTPase Arf1, an important regulator of the secretory pathway, is specifically required for dendrite pruning of ddaC/D/E sensory neurons but dispensable for apoptosis of ddaF neurons. Analyses of the GTP-and GDP-locked forms of Arf1 indicate that the cycling of Arf1 between GDP-bound and GTP-bound forms is essential for dendrite pruning. We further identified Sec71 as a guanine nucleotide exchange factor for Arf1 that preferentially interacts with its GDP-bound form. Like Arf1, Sec71 is also important for dendrite pruning, but not for apoptosis, of sensory neurons. Arf1 and Sec71 are interdependent for their localizations on Golgi. Finally, we show that the Sec71/Arf1-mediated trafficking process is a prerequisite for Rab5-dependent endocytosis to facilitate endocytosis and degradation of the cell-adhesion molecule Neuroglian (Nrg).

show abstract

Section: Sec71 Is An Arf1gef That Preferentially Interacts With Gdpbosupporting

confidence: 92%

Sec71 functions as a GEF for the small GTPase Arf1 to govern dendrite pruning of Drosophila sensory neurons

Wang¹,

Zhang²,

Shi³

et al. 2017

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…Indeed, a number of neuronal intrinsic factors have been identified that specifically regulate spacefilling of neurons. These factors include transcription factors that collectively determine the neuronal identity and bestow upon neurons the capacity to elaborate exuberant neurites (7,8), motor proteins and components of the secretary pathway that control the number and position of high-order dendrites (9)(10)(11)(12), and an amino acid transporter that allows neurons to grow total dendritic length beyond a certain threshold (13). In contrast to intrinsic control of dendritic growth, extracellular regulation of space-filling is more mysterious.…”

mentioning

confidence: 99%

Dendritic space-filling requires a neuronal type-specific extracellular permissive signal in Drosophila

Poe

Tang

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Neurons sometimes completely fill available space in their receptive fields with evenly spaced dendrites to uniformly sample sensory or synaptic information. The mechanisms that enable neurons to sense and innervate all space in their target tissues are poorly understood. Using somatosensory neurons as a model, we show that heparan sulfate proteoglycans (HSPGs) Dally and Syndecan on the surface of epidermal cells act as local permissive signals for the dendritic growth and maintenance of space-filling nociceptive C4da neurons, allowing them to innervate the entire skin. Using long-term time-lapse imaging with intact larvae, we found that dendrites grow into HSPG-deficient areas but fail to stay there. HSPGs are necessary to stabilize microtubules in newly formed high-order dendrites. In contrast to C4da neurons, non-space-filling sensory neurons that develop in the same microenvironment do not rely on HSPGs for their dendritic growth. Furthermore, HSPGs do not act by transporting extracellular diffusible ligands or require leukocyte antigen-related (Lar), a receptor protein tyrosine phosphatase (RPTP) and the only known HSPG receptor, for promoting dendritic growth of space-filling neurons. Interestingly, another RPTP, Ptp69D, promotes dendritic growth of C4da neurons in parallel to HSPGs. Together, our data reveal an HSPG-dependent pathway that specifically allows dendrites of space-filling neurons to innervate all target tissues in.

show abstract

“…The SIP is widely used to analyze differences and changes in dendritic morphologies (see, for example, Rekha et al, 2011;Williams et al, 2013;Peng et al, 2015;Johnson et al, 2016;Chittajallu et al, 2017;Keil et al, 2017). We have shown previously that the SIP can be accurately predicted from three factors with more functional interpretations: the dendrite spanning field, the centripetal bias, and the total length.…”

Section: Interpreting Changes In Sipmentioning

confidence: 99%

“…Fractal measures of neuronal morphology (Smith et al, 1989;Caserta et al, 1990), which quantify how well a dendrite fills its space, have also been shown to correlate strongly with statistics derived from the SIP (Caserta et al, 1995;Ferná ndez and Jelinek, 2001). Most recently, SIPs have been widely used to demonstrate changes in neuronal structure caused by genetic manipulation (O'Keeffe et al, 2008;Peng et al, 2015), pathology (Williams et al, 2013;Chittajallu et al, 2017), or treatment (Kigerl et al, 2009;Rekha et al, 2011).…”

Section: Introductionmentioning

confidence: 99%