Regulation of decellularized matrix mediated immune response

Chakraborty, Juhi; Roy, Subhadeep; Ghosh, Sourabh

doi:10.1039/c9bm01780a

Cited by 75 publications

(84 citation statements)

References 178 publications

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“…Additionally, the α-gal epitope, a carbohydrate responsible for the hyperacute rejection and failure of xenogeneic materials, was absent after decellularization (Macher and Galili, 2008;Pouliot et al, 2016). Then we monitored residual detergents (SDS; Naahidi et al, 2017;Chakraborty et al, 2020) and concentrations that do not interfere with cellular growth were achieved after six washes, similar to the data reported by Cebotari et al (2010).…”

Section: Discussionmentioning

confidence: 88%

“…The main biocompatibility concern in decellularized ECM materials is the possible presence of residual detergents, toxins, or cell debris (Lee et al, 2014;Keane et al, 2015;Naahidi et al, 2017;Chakraborty et al, 2020). In this proof of concept, we evaluated the overall acute immune response to EndoECM hydrogels and determined its toxic effects over a relatively short time span.…”

Section: Discussionmentioning

confidence: 99%

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A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

López-Martínez

Campo

Miguel-Gómez

et al. 2021

Front. Bioeng. Biotechnol.

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Decellularization techniques support the creation of biocompatible extracellular matrix hydrogels, providing tissue-specific environments for both in vitro cell culture and in vivo tissue regeneration. We obtained endometrium derived from porcine decellularized uteri to create endometrial extracellular matrix (EndoECM) hydrogels. After decellularization and detergent removal, we investigated the physicochemical features of the EndoECM, including gelation kinetics, ultrastructure, and proteomic profile. The matrisome showed conservation of structural and tissue-specific components with low amounts of immunoreactive molecules. EndoECM supported in vitro culture of human endometrial cells in two- and three-dimensional conditions and improved proliferation of endometrial stem cells with respect to collagen and Matrigel. Further, we developed a three-dimensional endometrium-like co-culture system of epithelial and stromal cells from different origins. Endometrial co-cultures remained viable and showed significant remodeling. Finally, EndoECM was injected subcutaneously in immunocompetent mice in a preliminary study to test a possible hypoimmunogenic reaction. Biomimetic endometrial milieus offer new strategies in reproductive techniques and endometrial repair and our findings demonstrate that EndoECM has potential for in vitro endometrial culture and as treatment for endometrial pathologies.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

López-Martínez

Campo

Miguel-Gómez

et al. 2021

Front. Bioeng. Biotechnol.

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“…The predictive plasticity and macrophage polarization on the decellularized scaffold surface help gain detailed information regarding the immunological response. 67,68 However, cell-mediated cytotoxicity plays an important role in the host immune defense. 69 The proliferation and cytotoxic assays are widely used for the biological potential determination of biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Development of biological meniscus scaffold: Decellularization method and recellularization with meniscal cell population derived from mesenchymal stem cells

et al. 2021

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Tissue engineering approaches which include a combination of cells and scaffold materials provide an alternative treatment for meniscus regeneration. Decellularization and recellularization techniques are potential treatment options for transplantation. Maintenance of the ultrastructure composition of the extracellular matrix and repopulation with cells are important factors in constructing a biological scaffold and eliminating immunological reactions. The aim of the study is to develop a method to obtain biological functional meniscus scaffolds for meniscus regeneration. For this purpose, meniscus tissue was decellularized by our modified method, a combination of physical, chemical, and enzymatic methods and then recellularized with a meniscal cell population composed of fibroblasts, chondrocytes and fibrochondrocytes that obtained from mesenchymal stem cells. Decellularized and recellularized meniscus scaffolds were analysed biochemically, biomechanically and histologically. Our results revealed that cellular components of the meniscus were successfully removed by preserving collagen and GAG structures without any significant loss in biomechanical properties. Recellularization results showed that the meniscal cells were localized in the empty lacuna on the decellularized meniscus, and also well distributed and proliferated consistently during the cell culture period (p < 0.05). Furthermore, a high amount of DNA, collagen, and GAG contents (p < 0.05) were obtained with the meniscal cell population in recellularized meniscus tissue. The study demonstrates that our decellularization and recellularization methods were effective to develop a biological functional meniscus scaffold and can mimic the meniscus tissue with structural and biochemical features. We predict that the obtained biological meniscus scaffolds may provide avoidance of adverse immune reactions and an appropriate microenvironment for allogeneic or xenogeneic recipients in the transplantation process. Therefore, as a promising candidate, the obtained biological meniscus scaffolds might be verified with a transplantation experiment.

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“…Despite the biochemical resemblance and complexity of musculoskeletal-tissue-derived dECM bioinks, there are limitations that must be overcome [152]. Usually, the innate and adaptive immune responses of the donor tissue to dECM are overlooked.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Park

Gao

Cho

2021

IJMS

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The musculoskeletal system is a vital body system that protects internal organs, supports locomotion, and maintains homeostatic function. Unfortunately, musculoskeletal disorders are the leading cause of disability worldwide. Although implant surgeries using autografts, allografts, and xenografts have been conducted, several adverse effects, including donor site morbidity and immunoreaction, exist. To overcome these limitations, various biomedical engineering approaches have been proposed based on an understanding of the complexity of human musculoskeletal tissue. In this review, the leading edge of musculoskeletal tissue engineering using 3D bioprinting technology and musculoskeletal tissue-derived decellularized extracellular matrix bioink is described. In particular, studies on in vivo regeneration and in vitro modeling of musculoskeletal tissue have been focused on. Lastly, the current breakthroughs, limitations, and future perspectives are described.

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Regulation of decellularized matrix mediated immune response

Abstract: This review addresses critical gaps and often-ignored aspects of immune response to a decellularized ECM.

Cited by 75 publications

References 178 publications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications

Development of biological meniscus scaffold: Decellularization method and recellularization with meniscal cell population derived from mesenchymal stem cells

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

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