“…On the other hand, it is well known that in hypo‐osmotic challenged cells, as a consequence of influx of water, cells swell and Mpk1p is transiently activated. In addition, it has also been reported that chitin synthesis is stimulated in cells grown in hypotonic conditions with respect to hypertonic ones (Davenport et al , 1995; Deshpande et al , 1997; Munro et al , 1998; Popolo et al , 1997; Turchini et al , 2000). In addition, it has been recently found that in cells defective in β(1,3)‐glucan synthesis, loss of downregulation of the HOG pathway or deletion of the glycerol channel Fps1p are detrimental for growth (Lesage et al , 2004).…”