Influence of sorbitol on protein production and glycosylation and cell wall formation in Trichoderma reesei

Górka-Nieć, Wioletta; Perlińska-Lenart, Urszula; Zembek, Patrycja; Palamarczyk, Grażyna; Kruszewska, Joanna S.

doi:10.1016/j.funbio.2010.07.010

Cited by 15 publications

(4 citation statements)

References 31 publications

(34 reference statements)

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“…Our studies demonstrated that osmotic protector reduces anti-Candida activity of 5h. In alignment with Górska-Nieć et al [35], we proved that enhanced biomass production leads to loss of antifungal activity of 5h at concentrations ranging from 4 to 16 µg/mL. Moreover, the activity of 5h did not correspond with AmB affecting cell wall due to activity accompanied by an increase concentration in medium with sorbitol [36].…”

Section: Discussionsupporting

confidence: 85%

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

et al. 2021

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Our study aimed to characterise the action mode of N-phenacyldibromobenzimidazoles against C. albicans and C. neoformans. Firstly, we selected the non-cytotoxic most active benzimidazoles based on the structure–activity relationships showing that the group of 5,6-dibromobenzimidazole derivatives are less active against C. albicans vs. 4,6-dibromobenzimidazole analogues (5e–f and 5h). The substitution of chlorine atoms to the benzene ring of the N-phenacyl substituent extended the anti-C. albicans action (5e with 2,4-Cl2 or 5f with 3,4-Cl2). The excellent results for N-phenacyldibromobenzimidazole 5h against the C. albicans reference and clinical isolate showed IC50 = 8 µg/mL and %I = 100 ± 3, respectively. Compound 5h was fungicidal against the C. neoformans isolate. Compound 5h at 160–4 µg/mL caused irreversible damage of the fungal cell membrane and accidental cell death (ACD). We reported on chitinolytic activity of 5h, in accordance with the patterns observed for the following substrates: 4-nitrophenyl-N-acetyl-β-d-glucosaminide and 4-nitrophenyl-β-d-N,N′,N″-triacetylchitothiose. Derivative 5h at 16 µg/mL: (1) it affected cell wall by inducing β-d-glucanase, (2) it caused morphological distortions and (3) osmotic instability in the C. albicans biofilm-treated. Compound 5h exerted Candida-dependent inhibition of virulence factors.

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Section: Discussionsupporting

confidence: 85%

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

et al. 2021

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“…As a result, cells exhibit limited activity of β-1,3-glucan synthase as well as chitin synthase. Sorbitol is therefore a factor that causes slight cell stress, which may cause the inhibition of cell growth in the presence of some nonspecific cell wall inhibitors [ 30 , 31 ]. As both cinnamaldehyde and sorbitol influence β-1,3-glucan synthase as well as chitin synthase activity, the decrease in MIC value for cinnamon oil in the presence of this osmoprotectant is explainable.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils

et al. 2018

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The antimicrobial activity of plant oils and extracts has been recognized for many years. In this study the activity of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus essential oils (EOs) distributed by Pollena Aroma (Nowy Dwór Mazowiecki, Poland) was investigated against a group of 183 clinical isolates of C. albicans and 76 isolates of C. glabrata. All of the oils exhibited both fungistatic and fungicidal activity toward C. albicans and C. glabrata isolates. The highest activity was observed for cinnamon oil, with MIC (Minimum Inhibitory Concentration) values in the range 0.002–0.125% (v/v). The MIC values of the rest of the oils were in the range 0.005% (or less) to 2.5% (v/v). In most cases MFC (Minimum Fungicidal Concentration) values were equal to MIC or twice as high. Additionally, we examined the mode of action of selected EOs. The effect on cell wall components could not be clearly proved. Three of the tested EOs (thyme, lemon, and clove) affected cell membranes. At the same time, thyme, cinnamon, and clove oil influenced potassium ion efflux, which was not seen in the case of lemon oil. All of the tested oils demonstrated the ability to inhibit the transition of yeast to mycelium form, but the effect was the lowest in the case of cinnamon oil.

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“…Finally, growth on high levels of sorbitol also resulted in growth reduction of the Δ Bcsun1 mutants ( Figure 2A ). This sugar alcohol is used frequently to stabilize osmotically damaged cell walls, but it may also increase glycerol production causing a reduction in the synthesis of cell wall components (Gorka-Niec et al, 2010). These findings strongly suggest that BcSUN1 may play a key role in the metabolism of the cell wall, since its absence in the mutants results in weaker walls.…”

Section: Discussionmentioning

confidence: 99%

BcSUN1, a B. cinerea SUN-Family Protein, Is Involved in Virulence

Pérez‐Hernández

González

et al. 2017

Front. Microbiol.

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BcSUN1 is a glycoprotein secreted by Botrytis cinerea, an important plant pathogen that causes severe losses in agriculture worldwide. In this work, the role of BcSUN1 in different aspects of the B. cinerea biology was studied by phenotypic analysis of Bcsun1 knockout strains. We identified BcSUN1 as the only member of the Group-I SUN family of proteins encoded in the B. cinerea genome, which is expressed both in axenic culture and during infection. BcSUN1 is also weakly attached to the cellular surface and is involved in maintaining the structure of the cell wall and/or the extracellular matrix. Disruption of the Bcsun1 gene produces different cell surface alterations affecting the production of reproductive structures and adhesion to plant surface, therefore reducing B. cinerea virulence. BcSUN1 is the first member of the SUN family reported to be involved in the pathogenesis of a filamentous fungus.

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Influence of sorbitol on protein production and glycosylation and cell wall formation in Trichoderma reesei

Cited by 15 publications

References 31 publications

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils

BcSUN1, a B. cinerea SUN-Family Protein, Is Involved in Virulence

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