Regulation of calcium-activated chloride channels by inositol 3,4,5,6 tetrakisphosphate

Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.)

“…Inositol phosphates have been suggested to participate in diverse cellular signaling. The levels of InsP 3 in many plants increased under treatment of hyperosmotic stress or drought, ABA, and cold Staxen et al 1999;Drobak and Watkins 2000;Munnik and Meijer 2001;Ruelland et al 2002;Hunt et al 2003).InsP 4 isomers play an important role in regulating ion channels such as Ca 2+ channels and Cl − channels (Irvine and Schell, 2001;Zonia et al, 2002;Ho and Shears, 2002). InsP 6 plays a major role in the response of the guard cells to ABA.…”

scyllo‐Inositol Pentakisphosphate as an Analogue of myo‐Inositol 1,3,4,5,6‐Pentakisphosphate: Chemical Synthesis, Physicochemistry and Biological Applications

“…Some delocalized electrostatic interactions of inositol phosphates with proteins might be biologically relevant. [10,11] However, other A C H T U N G T R E N N U N G delocalized electrostatic interactions, which can be permitted in a particular set of experimental conditions, might not be relevant to the intracellular milieu. [13] Based on earlier studies of other inositol phosphates, a larger difference between the acid-base properties of Ins(1,3,4,5,6)P 5 and scyllo-InsP 5 might have been expected.…”

“…This applies, for example, to Ca 2 + mobilization by InsA C H T U N G T R E N N U N G (1,4,5)P 3 [10] and Cl À channel inhibition by InsA C H T U N G T R E N N U N G (3,4,5,6)P 4 . [11] These ligand-protein interactions involve electrostatic interactions between the protein and the negatively charged phosphate groups on the ligand. Geometric constraints imposed by the bulky nature of the phosphate groups will also contribute to ligand specificity.…”

Molecular basis for the integration of inositol phosphate signaling pathways via human ITPK1

“…At least in mammalian cells, the inhibition of Cl − channel conductance by Ins(3,4,5,6) P 4 is an exquisitely specific regulatory process; it is not imitated by any of the many other inositol phosphates that exist inside cells (Ho et al, 2001; Ho and Shears, 2002; Xie et al, 1996). …”

“…Thus, Ins(3,4,5,6) P 4 is a molecule that has the potential to affect neuronal development. It therefore seems pertinent that Ins(3,4,5,6) P 4 has also previously been suggested to have the characteristics of a “memory molecule”, because its relatively slow rate of metabolism permits its physiological effects to long outlast the duration of the stimulus that initially prompts intracellular Ins(3,4,5,6) P 4 to accumulate (Ho and Shears, 2002). ClC3 is known to have many other roles, including tumor cell migration (Mao et al, 2008), bone remodeling (Okamoto et al, 2008), apoptosis (Claud et al, 2008), insulin secretion (Barg et al, 2001), and inflammatory responses (Moreland et al, 2006).…”