Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.)

Sui, Zhenhua; Niu, L. C.; Yue, Guidong; Yang, Aifang; Zhang, Juren

doi:10.1016/j.gene.2008.09.004

Cited by 18 publications

(39 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on this hypothesis, the present study was designed to explore the regulation and physiological function of the previously described ZmPIS gene (Sui et al 2008). We demonstrated that ZmPIS was able to complement a yeast mutant that contained a disrupted endogenous PIS1 gene (Fig.…”

Section: Discussionmentioning

confidence: 91%

“…This might help circumvent defects in the acylation synthesis pathway of PA (Athenstaedt and Daum 1999). Currently, plant PLCs and PLDs have been linked to salt and dehydration stresses in several independent studies (Hirayama et al 1995;Frank et al 2000;Munnik et al 2000;Katagiri et al 2001;Zhai et al 2005;Mane et al 2007;Hong et al 2008;Tasma et al 2008;Sui et al 2008;Wang et al 2008;Bargmann et al 2009). Consistent with this, PA plays a key role in the responses of plants to stresses, such as salinity, cold and drought, and several PA target proteins involved in biotic and/or abiotic stresses have been identified (Testerink and Munnik 2011).…”

Section: Introductionmentioning

confidence: 99%

“…We previously presented evidence that suggests the presence of at least two copies of the PIS gene in the maize (Zea mays L.) genome, ZmPIS and ZmPIS2 (Sui et al 2008). RT-PCR indicated that the expression of both PISs was upregulated in response to cold, drought and various phytohormones, such as abscisic acid, methyl jasmonate, indole-3-acetic acid and gibberellic acid.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance

Zhai

Gao

Hou

et al. 2011

Planta

View full text Add to dashboard Cite

Phosphatidylinositol (PtdIns) is an important lipid because it serves as a key membrane constituent and is the precursor of the inositol-containing lipids that are found in all plants and animals. It is synthesized from cytidine-diphosphodiacylglycerol (CDP-DG) and myo-inositol by PtdIns synthase (PIS). We have previously reported that two putative PIS genes from maize (Zea mays L.), ZmPIS and ZmPIS2, are transcriptionally up-regulated in response to drought (Sui et al., Gene, 426:47-56, 2008). In this work, we report on the characterization of ZmPIS in vitro and in vivo. The ZmPIS gene successfully complemented the yeast pis mutant BY4743, and the determination of PIS activity in the yeast strain further confirmed the enzymatic function of ZmPIS. An ESI-MS/MS-based lipid profiling approach was used to identify and quantify the lipid species in transgenic and wild-type tobacco plants before and after drought treatment. The results show that the overexpression of ZmPIS significantly increases lipid levels in tobacco leaves under drought stress compared to those of wild-type tobacco, which correlated well with the increased drought tolerance of the transgenic plants. Further analysis showed that, under drought stress conditions, ZmPIS overexpressors were found to exhibit increased membrane integrity, thereby enabling the retention of more solutes and water compared with the wild-type and the vector control transgenic lines. Our findings give us new insights into the role of the ZmPIS gene in the response of maize to drought/osmotic stress and the mechanisms by which plants adapt to drought stress.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance

Zhai

Gao

Hou

et al. 2011

Planta

View full text Add to dashboard Cite

show abstract

“…For real‐time RT‐PCR, the gene‐specific primer sequences of ZmPIS were used according to Sui et al . (). Real‐time RT‐PCR was performed on a ChromoTM 4 (MJ Research, Waltham, MA, USA) with the SYBR® RT‐PCR Kit (Takara, Dalian, China) following a method described by Sui et al .…”

Section: Methodsmentioning

confidence: 97%

Overexpression of the phosphatidylinositol synthase gene (ZmPIS) conferring drought stress tolerance by altering membrane lipid composition and increasing ABA synthesis in maize

Liu

Zhai²,

Zhao

et al. 2012

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Phosphatidylinositol (PtdIns) synthase is a key enzyme in the phospholipid pathway and catalyses the formation of PtdIns. PtdIns is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. Here, we obtained transgenic maize constitutively overexpressing or underexpressing PIS from maize (ZmPIS) under the control of a maize ubiquitin promoter. Transgenic plants were confirmed by PCR, Southern blotting analysis and realtime RT-PCR assay. The electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based lipid profiling analysis showed that, under drought stress conditions, the overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild type (WT). At the same time, the expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid (ABA) biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1 was markedly up-regulated in the overexpression lines after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants was enhanced significantly at the preflowering stages compared with WT maize plants. These results imply that ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize.

show abstract

“…Moreover, several genes have been shown to be upregulated in response to cold stress, including PLDα1, PLDδ, PLC1, PLC4, PLC5, DGK1 , and DGK2 (Hirayama et al, 1995; Gomez-Merino et al, 2004; Li et al, 2004; Lee et al, 2005). Also, in maize roots and leaves several genes encoding DGK, PLC, and PLD were upregulated within 30 min of cold stress (Sui et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase

Arisz¹,

Wijk²,

Roels³

et al. 2013

Front. Plant Sci.

427

560

View full text Add to dashboard Cite

Phosphatidic acid (PtdOH) is emerging as an important signaling lipid in abiotic stress responses in plants. The effect of cold stress was monitored using 32P-labeled seedlings and leaf discs of Arabidopsis thaliana. Low, non-freezing temperatures were found to trigger a very rapid 32P-PtdOH increase, peaking within 2 and 5 min, respectively. In principle, PtdOH can be generated through three different pathways, i.e., (1) via de novo phospholipid biosynthesis (through acylation of lyso-PtdOH), (2) via phospholipase D hydrolysis of structural phospholipids, or (3) via phosphorylation of diacylglycerol (DAG) by DAG kinase (DGK). Using a differential 32P-labeling protocol and a PLD-transphosphatidylation assay, evidence is provided that the rapid 32P-PtdOH response was primarily generated through DGK. A simultaneous decrease in the levels of 32P-PtdInsP, correlating in time, temperature dependency, and magnitude with the increase in 32P-PtdOH, suggested that a PtdInsP-hydrolyzing PLC generated the DAG in this reaction. Testing T-DNA insertion lines available for the seven DGK genes, revealed no clear changes in 32P-PtdOH responses, suggesting functional redundancy. Similarly, known cold-stress mutants were analyzed to investigate whether the PtdOH response acted downstream of the respective gene products. The hos1, los1, and fry1 mutants were found to exhibit normal PtdOH responses. Slight changes were found for ice1, snow1, and the overexpression line Super-ICE1, however, this was not cold-specific and likely due to pleiotropic effects. A tentative model illustrating direct cold effects on phospholipid metabolism is presented.

show abstract

Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.)

Cited by 18 publications

References 47 publications

Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance

Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance

Overexpression of the phosphatidylinositol synthase gene (ZmPIS) conferring drought stress tolerance by altering membrane lipid composition and increasing ABA synthesis in maize

Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase

Contact Info

Product

Resources

About