Regulation of Arabidopsis thaliana Ku genes at different developmental stages under heat stress

Liu, Pei‐Feng; Wang, Yung Kai; Chang, Wen Chi; Chang, Hwan‐You; Pan, Rong

doi:10.1016/j.bbagrm.2008.05.002

Cited by 10 publications

(8 citation statements)

References 38 publications

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“…uvh6 mutants have been reported to exhibit aberrant levels of several RNAs and proteins (Jenkins et al 1997; Liu et al 2008; Hall et al 2009). With respect to its role in heat tolerance, uvh6 was reported to contain increased levels of HSP21 (Jenkins et al 1997) but normal levels of HSP101 and sHSPs (Larkindale et al 2005).…”

Section: Resultsmentioning

confidence: 99%

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Kim

Hatherell

et al. 2012

G3 Genes|Genomes|Genetics

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Plants must adapt to a variety of abiotic inputs, including visible light, ultraviolet (UV) light, and heat. In Arabidopsis thaliana, DE-ETIOLATED 1 (DET1) plays a role in visible light signaling, UV tolerance, and development. UV-HYPERSENSITIVE 6 (UVH6) mutants are UV and heat sensitive, as well as dwarf and pale, like det1. In this study, we examine the genetic interactions between these two genes. In dark-grown seedlings, uvh6 exhibits a weak de-etiolated phenotype but does not affect the stronger de-etiolated phenotype of det1. In the light, det1 is epistatic to uvh6 with regard to chlorophyll level, but their effect on all size parameters is additive and therefore independent. With regard to UV tolerance, det1 UV resistance is epistatic to uvh6 UV sensitivity. In heat stress experiments, det1 enhances heat-induced tissue damage in the uvh6 background but suppresses heat-induced growth inhibition. Thus, det1 acts epistatically to uvh6 with respect to de-etiolation, chlorophyll level, UV tolerance, and heat-induced growth inhibition, whereas det1 and uvh6 act additively to regulate plant size and heat-induced cell death. These data provide insight into interplay between light and heat signaling.

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Section: Resultsmentioning

confidence: 99%

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Kim

Hatherell

et al. 2012

G3 Genes|Genomes|Genetics

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“…The seeds were harvested from mature dry siliques and stored in darkness at room temperature. The transferred DNA insertion mutants lacking either AT4G13250 (SALK_091664; NYC1) or AT3G24650 (SALK_138922; ABI3 [Liu et al, 2008]) were obtained from the Arabidopsis Biological Resource Center. The transferred DNA insertion mutants lacking AT5G04900 (AL759262; NOL) were obtained from GABI-Kat.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Chlorophyll b Reductase Plays an Essential Role in Maturation and Storability of Arabidopsis Seeds

Nakajima

Itô²,

Tanaka³

et al. 2012

Plant Physiology

108

104

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Although seeds are a sink organ, chlorophyll synthesis and degradation occurs during embryogenesis and in a manner similar to that observed in photosynthetic leaves. Some mutants retain chlorophyll after seed maturation, and they are disturbed in seed storability. To elucidate the effects of chlorophyll retention on the seed storability of Arabidopsis (Arabidopsis thaliana), we examined the non-yellow coloring1 (nyc1)/nyc1-like (nol) mutants that do not degrade chlorophyll properly. Approximately 10 times more chlorophyll was retained in the dry seeds of the nyc1/nol mutant than in the wild-type seeds. The germination rates rapidly decreased during storage, with most of the mutant seeds failing to germinate after storage for 23 months, whereas 75% of the wild-type seeds germinated after 42 months. These results indicate that chlorophyll retention in the seeds affects seed longevity. Electron microscopic studies indicated that many small oil bodies appeared in the embryonic cotyledons of the nyc1/nol mutant; this finding indicates that the retention of chlorophyll affects the development of organelles in embryonic cells. A sequence analysis of the NYC1 promoter identified a potential abscisic acid (ABA)-responsive element. An electrophoretic mobility shift assay confirmed the binding of an ABA-responsive transcriptional factor to the NYC1 promoter DNA fragment, thus suggesting that NYC1 expression is regulated by ABA. Furthermore, NYC1 expression was repressed in the ABAinsensitive mutants during embryogenesis. These data indicate that chlorophyll degradation is induced by ABA during seed maturation to produce storable seeds.

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“…ABA has been found to repress the expression of both Ku80 and Ku70 following heat stress, which causes higher accumulation of ABA in plants [69]. In another study, UV-sensitive Arabidopsis mutant uvs66 was shown to be hypersensitive to salinity and ABA.…”

Section: Discussionmentioning

confidence: 99%

Homologous Recombination Defective Arabidopsis Mutants Exhibit Enhanced Sensitivity to Abscisic Acid

Roy

Das

2017

PLoS ONE

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Abscisic acid (ABA) acts as an important plant hormone in regulating various aspects of plant growth and developmental processes particularly under abiotic stress conditions. An increased ABA level in plant cells inhibits DNA replication and cell division, causing plant growth retardation. In this study, we have investigated the effects of ABA on the growth responses of some major loss-of-function mutants of DNA double-stand break (DSB) repair genes in Arabidopsis during seed germination and early stages of seedling growth for understanding the role of ABA in the induction of genome instability in plants. A comparative analysis of ABA sensitivity of wild-type Arabidopsis and the knockout mutant lines related to DSB sensors, including atatm, atatr, the non-homologous end joining (NHEJ) pathway genes, and mutants related to homologous recombination (HR) pathway genes showed relatively enhanced sensitivity of atatr and HR-related mutants to ABA treatment. The expression levels of HR-related genes were increased in wild-type Arabidopsis (Col-0) during seed germination and early stages of seedling growth. Immunoblotting experiments detected phosphorylation of histone H2AX in wild-type (Col-0) and DSB repair gene mutants after ABA treatment, indicating the activation of DNA damage response due to ABA treatment. Analyses of DSB repair kinetics using comet assay under neutral condition have revealed comparatively slower DSB repair activity in HR mutants. Overall, our results have provided comprehensive information on the possible effect of ABA on DNA repair machinery in plants and also indicated potential functional involvement of HR pathway in repairing ABA induced DNA damage in Arabidopsis.

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Regulation of Arabidopsis thaliana Ku genes at different developmental stages under heat stress

Cited by 10 publications

References 38 publications

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Genetic Interactions Between ArabidopsisDET1andUVH6During Development and Abiotic Stress Response

Chlorophyll b Reductase Plays an Essential Role in Maturation and Storability of Arabidopsis Seeds

Homologous Recombination Defective Arabidopsis Mutants Exhibit Enhanced Sensitivity to Abscisic Acid

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