Homologous Recombination Defective Arabidopsis Mutants Exhibit Enhanced Sensitivity to Abscisic Acid

Roy, Sujit; Das, K. P.

doi:10.1371/journal.pone.0169294

Cited by 10 publications

(2 citation statements)

References 75 publications

(115 reference statements)

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“…Indeed, both seed dehydration and germination, which are accompanied by a burst of ROS production, are highly damaging for DNA, and up-regulation of DNA repair genes during germination is well documented in Arabidopsis (Waterworth et al, 2010), Medicago truncatula (Balestrazzi et al, 2011) and Phaseolus vulgaris (Parreira et al, 2018). Consistently, atm mutants fail to delay germination in aged seeds, and show extensive chromosomal abnormalities (Waterworth et al, 2016), and HR-deficient or DDR mutants are hypersensitive to ABA during germination and at the seedling stage (Roy and Das, 2017). Thus, the probable contribution of the plant DDR to abiotic stress tolerance is supported by its essential role during germination, a particularly stressful step of the plant life cycle.…”

Section: Role Of the Plant Ddr In Abiotic Stress Responsesmentioning

confidence: 86%

The Plant DNA Damage Response: Signaling Pathways Leading to Growth Inhibition and Putative Role in Response to Stress Conditions

et al. 2019

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Maintenance of genome integrity is a key issue for all living organisms. Cells are constantly exposed to DNA damage due to replication or transcription, cellular metabolic activities leading to the production of Reactive Oxygen Species (ROS) or even exposure to DNA damaging agents such as UV light. However, genomes remain extremely stable, thanks to the permanent repair of DNA lesions. One key mechanism contributing to genome stability is the DNA Damage Response (DDR) that activates DNA repair pathways, and in the case of proliferating cells, stops cell division until DNA repair is complete. The signaling mechanisms of the DDR are quite well conserved between organisms including in plants where they have been investigated into detail over the past 20 years. In this review we summarize the acquired knowledge and recent advances regarding the DDR control of cell cycle progression. Studying the plant DDR is particularly interesting because of their mode of development and lifestyle. Indeed, plants develop largely post-embryonically, and form new organs through the activity of meristems in which cells retain the ability to proliferate. In addition, they are sessile organisms that are permanently exposed to adverse conditions that could potentially induce DNA damage in all cell types including meristems. In the second part of the review we discuss the recent findings connecting the plant DDR to responses to biotic and abiotic stresses.

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Section: Role Of the Plant Ddr In Abiotic Stress Responsesmentioning

confidence: 86%

The Plant DNA Damage Response: Signaling Pathways Leading to Growth Inhibition and Putative Role in Response to Stress Conditions

et al. 2019

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“…Further studies identified a potential connection between the stress-induced hormone abscisic acid (ABA) and HR ( Roy and Das, 2017 ). Exogenous treatment of ABA enhances HR frequency, and plants with knockout mutations in the HR-related genes AtRAD51 , AtRAD52 , AtRAD54, and AtBRCA1 display an ABA hypersensitive phenotype in seed germination ( Roy and Das, 2017 ). Furthermore, the ABA hypersensitive mutant, abo4-1 , exhibits a 60-fold increase in HR frequency relative to wild type ( Yin et al, 2009 ).…”

Section: Other Factors That Influence Plant Hr Frequencymentioning

confidence: 99%

Enhancing HR Frequency for Precise Genome Editing in Plants

2022

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Gene-editing tools, such as Zinc-fingers, TALENs, and CRISPR-Cas, have fostered a new frontier in the genetic improvement of plants across the tree of life. In eukaryotes, genome editing occurs primarily through two DNA repair pathways: non-homologous end joining (NHEJ) and homologous recombination (HR). NHEJ is the primary mechanism in higher plants, but it is unpredictable and often results in undesired mutations, frameshift insertions, and deletions. Homology-directed repair (HDR), which proceeds through HR, is typically the preferred editing method by genetic engineers. HR-mediated gene editing can enable error-free editing by incorporating a sequence provided by a donor template. However, the low frequency of native HR in plants is a barrier to attaining efficient plant genome engineering. This review summarizes various strategies implemented to increase the frequency of HDR in plant cells. Such strategies include methods for targeting double-strand DNA breaks, optimizing donor sequences, altering plant DNA repair machinery, and environmental factors shown to influence HR frequency in plants. Through the use and further refinement of these methods, HR-based gene editing may one day be commonplace in plants, as it is in other systems.

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Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

Sihi

Bakshi

Maiti

et al. 2021

J Plant Growth Regul

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Homologous Recombination Defective Arabidopsis Mutants Exhibit Enhanced Sensitivity to Abscisic Acid

Cited by 10 publications

References 75 publications

The Plant DNA Damage Response: Signaling Pathways Leading to Growth Inhibition and Putative Role in Response to Stress Conditions

The Plant DNA Damage Response: Signaling Pathways Leading to Growth Inhibition and Putative Role in Response to Stress Conditions

Enhancing HR Frequency for Precise Genome Editing in Plants

Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

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