Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

Sihi, Sayantani; Bakshi, Sankar; Maiti, Satyabrata; Nayak, Arup; Sengupta, Dibyendu N.

doi:10.1007/s00344-021-10390-7

Cited by 8 publications

(4 citation statements)

References 71 publications

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“…Drought can cause damage to plants’ cellular structures, such as membrane lipids, proteins, and DNA, which is often caused by the accumulation of reactive oxygen species (ROS) [ 65 ]. Levels of MDA and H 2 O 2 are widely measured to assess the production of ROS in plants [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Humic Substances and Mycorrhizal Fungi on Drought-Stressed Cactus: Focus on Growth, Physiology, and Biochemistry

Lahbouki,

Fernando,

Rodrigues

et al. 2023

Plants

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Utilizing water resources rationally has become critical due to the expected increase in water scarcity. Cacti are capable of surviving with minimal water requirements and in poor soils. Despite being highly drought-resistant, cacti still faces limitations in realizing its full potential under drought-stress conditions. To this end, we investigated the interactive effect of humic substances (Hs) and arbuscular mycorrhizal fungi (AMF) on cactus plants under drought stress. In the study, a cactus pot experiment had three irrigation levels (W1: no irrigation, W2: 15% of field capacity, and W3: 30% of field capacity) and two biostimulants (Hs soil amendment and AMF inoculation), applied alone or combined. The findings show that the W1 and W2 regimes affected cactus performance. However, Hs and/or AMF significantly improved growth. Our results revealed that drought increased the generation of reactive oxygen species. However, Hs and/or AMF application improved nutrient uptake and increased anthocyanin content and free amino acids. Furthermore, the soil’s organic matter, phosphorus, nitrogen, and potassium contents were improved by the application of these biostimulants. Altogether, using Hs alone or in combination with AMF can be an effective and sustainable approach to enhance the tolerance of cactus plants to drought conditions, while also improving the soil quality.

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Section: Discussionmentioning

confidence: 99%

Effects of Humic Substances and Mycorrhizal Fungi on Drought-Stressed Cactus: Focus on Growth, Physiology, and Biochemistry

Lahbouki,

Fernando,

Rodrigues

et al. 2023

Plants

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“…Salinity-induced ROS-dependent DNA damage and alteration in plants have not been extensively studied ( Huh et al., 2002 ; Demidchik et al., 2010 ). According to research by Sihi et al. (2022) , the main mechanism by which salinity stress causes DNA strand breaks in rice is the release of ROS.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the genotoxicity of salinity stress and secondary products gene manipulation in lime, Citrus aurantifolia, plants

et al. 2023

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Salinity is a significant abiotic stress that has a profound effect on growth, the content of secondary products, and the genotoxicity of cells. Lime, Citrus aurantifolia, is a popular plant belonging to the family Rutaceae. The interest in cultivating this plant is due to the importance of its volatile oil, which is included in many pharmaceutical industries, but C. aurantifolia plants are affected by the NaCl salinity levels. In the present study, a comet assay test has been applied to evaluate the genotoxic impact of salinity at 0, 50, 100, and 200 mM of NaCl on C. aurantifolia tissue-cultured plants. Furthermore, terpene gene expression was investigated using a semi-quantitative real-time polymerase chain reaction. Results from the two analyses revealed that 200 mM of NaCl stress resulted in high levels of severe damage to the C. aurantifolia plants’ DNA tail 21.8%, tail length 6.56 µm, and tail moment 3.19 Unit. The relative highest expression of RtHK and TAT genes was 2.08, and 1.693, respectively, when plants were exposed to 200 mM of NaCl, whereas pv4CL2RT expressed 1.50 in plants subjected to 100 mM of NaCl. The accumulation of transcripts for the RTMYB was 0.951 when plants were treated with NaCl at 50 mM, and RtGPPS gene was significantly decreased to 0.446 during saline exposure at 100 mM. We conclude that the comet assay test offers an appropriate tool to detect DNA damage as well as RtHK, TAT, and pv4CL2RT genes having post-transcriptional regulation in C. aurantifolia plant cells under salinity stress. Future studies are needed to assess the application of gene expression and comet assay technologies using another set of genes that show vulnerability to different stresses on lime and other plants.

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“…Heavy metals roots of Brassica juncea alkaline CASP software [166] roots of Brassica juncea alkaline Carl Zeiss Axiovision software (Special Edition 64 Rel. 4.9.1) [27] leaves of Vitis vinifera alkaline visual scoring (four classes of damaged DNA) followed by FISH [28] roots of Vicia faba and Allium cepa alkaline Comet Assay IV (Perceptive Instruments) [167] leaves of 30-day-old Solanum lycopersicum alkaline CASP software [182] root tips of Allium cepa neutral Comet Assay Software (CASP-version 1.2.3b) [29] root tips of Lupinus luteus alkaline Scion Image analysis system [165] the roots and shoots of Cucumis melo seedlings alkaline visual scoring and ranking into classes 0-4 (non-damagedmaximally damaged) [169] High [190] root tip cells and 7 d old seedlings of Hordeum vulgare alkaline OpenComet v1.3.1 Software [191] leaves of 32-day-old Vicia faba alkaline Komet 5 software (Liverpoo1, UK) [192] roots of Allium cepa alkaline Carl Zeiss Axiovision software [18] germinated seeds of Solanum melongena neutral CaspLab software [193] protonema [195] root tips of Lactuca sativa seedlings alkaline CASP software [22] roots of Lactuca sativa seedlings alkaline scoring with the classification of comets into 0 to 4 according to shape [23] Neutral Comet assays performed with the leaves of barley genotypes with different salt tolerance showed that after two weeks of plant growth in a hydroponic medium supplemented with 300 mM NaCl, DNA damage in leaf cells of a salt-sensitive cultivar significantly increased; this was accompanied by a rise in ROS production and a drop in the activities of antioxidant systems [188].…”

Section: Use Of the Comet Assay In Studies On Plants Exposed To Cytot...mentioning

confidence: 99%

“…The role of DNA polymerase λ (OsPolλ) in DNA repair and in plant tolerance to salinity and drought stress was studied on three Oryza sativa cultivars [190]. The damage to genomic DNA was assessed using neutral Comet assays.…”

Section: Use Of the Comet Assay In Studies On Plants Exposed To Cytot...mentioning

confidence: 99%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

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Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

Cited by 8 publications

References 71 publications

Effects of Humic Substances and Mycorrhizal Fungi on Drought-Stressed Cactus: Focus on Growth, Physiology, and Biochemistry

Effects of Humic Substances and Mycorrhizal Fungi on Drought-Stressed Cactus: Focus on Growth, Physiology, and Biochemistry

Evaluating the genotoxicity of salinity stress and secondary products gene manipulation in lime, Citrus aurantifolia, plants

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

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