Regulation of angiopoietin-like protein 4 production during and after exercise

Norheim, Frode; Hjorth, Marit; Langleite, Torgrim M.; Lee, Sindre; Holen, Torgeir; Bindesbøll, Christian; Stadheim, Hans Kristian; Gulseth, Hanne Løvdal; Birkeland, Kåre I.; Kielland, Anders; Jensen, Jørgen; Dalen, Knut Tomas; Drevon, Christian A.

doi:10.14814/phy2.12109

Cited by 43 publications

(54 citation statements)

References 33 publications

(108 reference statements)

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“…However, a chronic exercise intervention combining strength and endurance training for 12 weeks had no effect on basal plasma ANGPTL4 concentrations and skeletal muscle ANGPTL4 mRNA content. 52 In conclusion, ANGPTL4 is highly induced in muscle in response to exercise. However, adipose tissue and the liver may contribute more than muscle to the exerciseinduced increase in circulating ANGPTL4.…”

Section: Skeletal Muscle As the Source Of Myokines And Adipo-myokinesmentioning

confidence: 88%

“…In a study by Norheim et al, it was reported that 30 min after an acute bout of endurance exercise, ANGPTL4 mRNA expression was significantly higher in overweight dysglycemic subjects compared to lean controls at the beginning of the intervention study. 52 Interestingly, they found that ANGPTL4 mRNA expression was much higher in adipose tissue than muscle. Following 12 weeks of combined endurance and resistance training, adipose tissue mRNA expression of ANGPTL4 as well as basal plasma levels was unchanged in both groups.…”

Section: Exercise and The Production Of Adipokines By Adipose Tissuementioning

confidence: 99%

“…However, adipose tissue and the liver may contribute more than muscle to the exerciseinduced increase in circulating ANGPTL4. 52 The chemokine MCP-1 is another adipo-myokine which is regulated by exercise in muscle. Among other functions, it plays an important role in the recruitment of monocytes and T lymphocytes into tissues.…”

Section: Skeletal Muscle As the Source Of Myokines And Adipo-myokinesmentioning

confidence: 99%

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Exercise and Regulation of Adipokine and Myokine Production

Görgens

Eckardt

Jensen

et al. 2015

Progress in Molecular Biology and Translational Science

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127

108

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Section: Skeletal Muscle As the Source Of Myokines And Adipo-myokinesmentioning

confidence: 88%

Section: Exercise and The Production Of Adipokines By Adipose Tissuementioning

confidence: 99%

Section: Skeletal Muscle As the Source Of Myokines And Adipo-myokinesmentioning

confidence: 99%

See 1 more Smart Citation

Exercise and Regulation of Adipokine and Myokine Production

Görgens

Eckardt

Jensen

et al. 2015

Progress in Molecular Biology and Translational Science

Self Cite

127

108

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“…RNA integrity was determined using Agilent RNA 6000 Nano Chips and a Bioanalyzer 2100. Deep sequencing was performed with the Illumina HiSeq 2000 system with multiplexed design [22]. The cDNA was fragmented, and cDNA fragments with 51 bp nucleotides were selected and amplified.…”

Section: Methodsmentioning

confidence: 99%

Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines

Pourteymour

Eckardt

Holen

et al. 2017

Molecular Metabolism

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ObjectiveSkeletal muscle is an important secretory organ, producing and releasing numerous myokines, which may be involved in mediating beneficial health effects of physical activity. More than 100 myokines have been identified by different proteomics approaches, but these techniques may not detect all myokines. We used mRNA sequencing as an untargeted approach to study gene expression of secreted proteins in skeletal muscle upon acute as well as long-term exercise.MethodsTwenty-six middle-aged, sedentary men underwent combined endurance and strength training for 12 weeks. Skeletal muscle biopsies from m. vastus lateralis and blood samples were taken before and after an acute bicycle test, performed at baseline as well as after 12 weeks of training intervention. We identified transcripts encoding secretory proteins that were changed more than 1.5-fold in muscle after exercise. Secretory proteins were defined based on either curated UniProt annotations or predictions made by multiple bioinformatics methods.ResultsThis approach led to the identification of 161 candidate secretory transcripts that were up-regulated after acute exercise and 99 that where increased after 12 weeks exercise training. Furthermore, 92 secretory transcripts were decreased after acute and/or long-term physical activity. From these responsive transcripts, we selected 17 candidate myokines sensitive to short- and/or long-term exercise that have not been described as myokines before. The expression of these transcripts was confirmed in primary human skeletal muscle cells during in vitro differentiation and electrical pulse stimulation (EPS). One of the candidates we identified was macrophage colony-stimulating factor-1 (CSF1), which influences macrophage homeostasis. CSF1 mRNA increased in skeletal muscle after acute and long-term exercise, which was accompanied by a rise in circulating CSF1 protein. In cultured muscle cells, EPS promoted a significant increase in the expression and secretion of CSF1.ConclusionWe identified 17 new, exercise-responsive transcripts encoding secretory proteins. We further identified CSF1 as a novel myokine, which is secreted from cultured muscle cells and up-regulated in muscle and plasma after acute exercise.

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“…The expression level of ANGPTL‐4 depends on nutritional status and human plasma ANGPTL‐4 increases after long‐term fasting, chronic caloric restriction, and following physical activity . These three situations have as a common feature of elevated plasma FFA levels.…”

Section: Introductionmentioning

confidence: 99%