Regulation of Androgen Receptor-Mediated Transcription by RPB5 Binding Protein URI/RMP

Mita, Paolo; Savas, Jeffrey N.; Djouder, Nabil; Yates, John R.; Ha, Susan; Ruoff, Rachel; Schafler, Eric D.; Nwachukwu, Jerome C.; Tanese, Naoko; Cowan, Nicholas J.; Zavadil, Jiří; Garabedian, Michael J.; Logan, Susan K.

doi:10.1128/mcb.05429-11

Cited by 32 publications

(60 citation statements)

References 34 publications

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“…Surprisingly, we also found that AR binds to ARE I in the coding region and not in the promoter. We have also demonstrated that URI depletion results in decreased Art-27 recruitment to the TSS and increased AR recruitment to ARE1 (Mita et al, 2011).…”

Section: Sub-aim 1asupporting

confidence: 52%

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URI Regulation of Androgen Receptor-Mediated Cell Growth

Schafler¹,

Logan²

2013

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Section: Sub-aim 1asupporting

confidence: 52%

“…To gain a better understanding of the role of the AR/Art-27/URI transcription complex in mediating gene transcription, we performed ChIP analysis in LNCaP cells to locate each of these factors along the promoter and coding region of Nkx3.1 (Mita et al, 2011).…”

Section: Sub-aim 1amentioning

confidence: 99%

URI Regulation of Androgen Receptor-Mediated Cell Growth

Schafler¹,

Logan²

2013

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“…Tip60 and RVB1/2 are also recruited by c-myc to its target promoters, and Tip60 is required for AR-and p53-mediated transcription (40,41). Yet another RVB-containing complex, URI, negatively regulates RNA Pol II during activated transcription driven by VP16 and hepatitis B virus X protein (34), and it was recently shown to negatively regulate AR-driven transcription (33). Although these complexes contain RVB proteins, the role of these proteins in regulating many of these processes remains unexplored.…”

Section: Discussionmentioning

confidence: 99%

“…The URI complex contains RVB proteins (32) and is known to negatively modulate VP16-and androgen receptor (AR)-driven transcription (33,34). Two doxycyclineinducible hairpins against URI efficiently decreased URI protein levels (Fig.…”

Section: Gene Expression Analysis Of Hela Cells Carrying Tagged Stat2mentioning

confidence: 99%

The Human RVB Complex Is Required for Efficient Transcription of Type I Interferon-Stimulated Genes

Gnatovskiy

Mita

Levy

2013

Molecular and Cellular Biology

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Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosinephosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA ؉ ATP binding proteins contained in chromatin-remodeling complexes such as Ino80, SNF2-related CBP activator protein (SRCAP), and Tip60/NuA4, interacted with the transactivation domain of STAT2 in the nuclei of IFN-stimulated cells. RNA interference (RNAi) experiments demonstrated that RVB proteins were required for robust activation of IFN-␣-stimulated genes (ISGs). The requirement for RVB proteins was specific to IFN-␣/ STAT2 signaling; transcription of tumor necrosis factor alpha (TNF-␣)-and IFN-␥-driven genes was not affected by RVB1 depletion. Using RNAi-based depletion, we assessed the involvement of catalytic subunits of the RVB-containing Tip60, BRD8, Ino80, SRCAP, and URI complexes. No component other than RVB1/2 was uniquely required for ISG induction, suggesting that RVB1/2 functions as part of an as yet unidentified complex. Chromatin immunoprecipitation assays indicated that RVB1/2 was required for recruitment of RNA polymerase II (Pol II) to ISG promoters but was dispensable for STAT2 recruitment to chromatin. We hypothesize that an RVB1/2 chromatin-remodeling complex is required for efficient Pol II recruitment and initiation at ISG promoters and is recruited through interaction with the STAT2 transactivation domain. Interferons (IFNs) are a family of pleiotropic cytokines primarily known for their ability to establish a potent antiviral state via modulation of the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signal transduction pathway. Alternatively, aberrant production of IFNs correlates with systemic autoimmunity, underscoring the importance of the stringent regulation of IFN responses. Type I IFNs activate the heterotrimeric transcription factor ISGF3, composed of tyrosine-phosphorylated STAT1 and STAT2 and an auxiliary DNA binding protein, IRF9. Following IFN stimulation, phosphorylated ISGF3 translocates into the nucleus, binds the interferon-stimulated response elements (ISREs) in the promoters of IFN-␣/␤-stimulated genes (ISGs), and rapidly and robustly induces the transcription of a large family of previously silent genes. ISGs subsequently influence multiple cellular pathways involved in innate immunity in order to protect against viral and bacterial replication, including modulation of cell stress responses, apoptosis, proliferation, translation, and innate and adaptive immune signaling (1). In the context of ISGF3, STAT2 contains an essential transactivation domain (TAD) that provides most of the transcriptional function to the transcription factor complex, whereas STAT1 and IRF9 confer DNA sequence specificity (2). Induction of IFN target genes provides a robust system for studying the molecular mechanisms underlying transcriptional regulation. However, little is...

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“…Owing to RPB5-URI interaction, RPB5 could participate in regulating the androgen receptor in human cells [41]. This interaction also extends to S. cerevisiae and the correct association between Rpb5 and the URI orthologue, Bud27, is essential for the correct cytoplasmic assembly of the three RNA pols before their entry to the nucleus [42].…”

Section: Rpb5mentioning

confidence: 99%

Subunits Common to RNA Polymerases

Cuevas-Bermúdez¹,

Martínez-Fernández²,

Garrido-Godino³

et al. 2018

The Yeast Role in Medical Applications

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RNA polymerases are heteromultimeric complexes responsible of RNA synthesis. In yeast, as in the other eukaryotes, these complexes contain five common subunits (Rpb5, Rpb6, Rpb8, Rpb10 and Rpb12) that must have similar functions in the three RNA polymerases. However, some of these proteins have been shown to also have specific roles. In the last few decades, substantial progress has been made to understand the role of these common subunits in transcription, but their participation in the activity of each enzyme remains unclear. This review gives a comprehensive overview of current knowledge on the five common subunits of eukaryotic RNA pol, placing attention not only on their common roles in the activity of the RNA pols but also on describing specific roles for some of the complexes.

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Regulation of Androgen Receptor-Mediated Transcription by RPB5 Binding Protein URI/RMP

Cited by 32 publications

References 34 publications

URI Regulation of Androgen Receptor-Mediated Cell Growth

URI Regulation of Androgen Receptor-Mediated Cell Growth

The Human RVB Complex Is Required for Efficient Transcription of Type I Interferon-Stimulated Genes

Subunits Common to RNA Polymerases

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