Regulation of aging by unc-13 and sbt-1 in Caenorhabditis elegans is temperature-dependent

He, Ke-Wen; Shen, Lanxiao; Zhou, Wenwen; Wang, Dayong

doi:10.1007/s12264-009-6123-2

Cited by 15 publications

(6 citation statements)

References 30 publications

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“…Based on studies of ortholog genes in C. elegans aging models, it has been suggested that some neurexins also contribute to the process of aging [19]. Here, we investigated the NRX-1 gene, the C. elegans ortholog of the mammalian CNTNAP4 gene [20].…”

Section: Resultsmentioning

confidence: 99%

A Common Copy Number Variation (CNV) Polymorphism in the CNTNAP4 Gene: Association with Aging in Females

Iakoubov¹,

Mossakowska

Szwed

et al. 2013

PLoS ONE

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BackgroundAging is a biological process strongly determined by genetics. However, only a few single nucleotide polymorphisms (SNPs) have been reported to be consistently associated with aging. While investigating whether copy number variations (CNVs) could fill this gap, we focused on CNVs that have not been studied in previous SNP-based searches via tagging SNPs.MethodsTaqMan qPCR assays were developed to quantify 20 common CNVs in 222 senior American Caucasians in order to reveal possible association with longevity. The replication study was comprised of 1283 community-dwelling senior European Caucasians. Replicated CNVs were further investigated for association with healthy aging and aging-related diseases, while association with longevity was additionally tested in Caenorhabditis elegans.ResultsIn the discovery study of ≥80 vs.<80 years old seniors, a homozygous intronic CNV deletion in the CNTNAP4 gene was inversely associated with survival to the age of 80 (OR=0.51, 95%CI 0.29-0.87, p=0.015 before correction for multiple testing). After stratification by sex, association remained significant in females (OR=0.41, 95%CI 0.21-0.77, p=0.007), but not in males (OR=0.97, 95%CI 0.33-2.79, p=1). The finding was validated in a replication study (OR=0.66, 95%CI 0.48-0.90, p=0.011 for females). CNTNAP4 association with longevity was supported by a marked 25% lifespan change in C. elegans after knocking down the ortholog gene. An inverse association of the CNV del/del variant with female healthy aging was observed (OR=0.39, 95%CI 0.19-0.76, p=0.006). A corresponding positive association with aging-related diseases was revealed for cognitive impairment (OR=2.17, 95%CI 1.11-4.22, p=0.024) and, in independent studies, for Alzheimer’s (OR=4.07, 95%CI 1.17-14.14, p=0.036) and Parkinson’s (OR=1.59, 95%CI 1.03-2.42, p=0.041) diseases.ConclusionThis is the first demonstration for association of the CNTNAP4 gene and one of its intronic CNV polymorphisms with aging. Association with particular aging-related diseases awaits replication and independent validation.

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Section: Resultsmentioning

confidence: 99%

A Common Copy Number Variation (CNV) Polymorphism in the CNTNAP4 Gene: Association with Aging in Females

Iakoubov¹,

Mossakowska

Szwed

et al. 2013

PLoS ONE

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“…Lifespan assay was performed basically as described [65] – [66] . In the test, the hermaphrodites were transferred daily for the first 4 days of adulthood.…”

Section: Methodsmentioning

confidence: 99%

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Zhuang

Zhao

et al. 2014

PLoS ONE

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In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.

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“…Lifespan assay was performed as previously described (He et al, 2009;Wang et al, 2013). Hermaphrodites were transferred daily for the first 4 days of adulthood.…”

Section: Lifespan Assaymentioning

confidence: 99%

Adverse effects of coal combustion related fine particulate matter (PM 2.5 ) on nematode Caenorhabditis elegans

Sun

Liu²,

Liao

et al. 2015

Science of The Total Environment

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Regulation of aging by unc-13 and sbt-1 in Caenorhabditis elegans is temperature-dependent

Cited by 15 publications

References 30 publications

A Common Copy Number Variation (CNV) Polymorphism in the CNTNAP4 Gene: Association with Aging in Females

A Common Copy Number Variation (CNV) Polymorphism in the CNTNAP4 Gene: Association with Aging in Females

Adverse Effects from Clenbuterol and Ractopamine on Nematode Caenorhabditis elegans and the Underlying Mechanism

Adverse effects of coal combustion related fine particulate matter (PM 2.5 ) on nematode Caenorhabditis elegans

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