Regulation of Adenovirus Gene Expression

Persson, Håkan; Philipson, Lennart

doi:10.1007/978-3-642-68318-3_4

Cited by 20 publications

(10 citation statements)

References 245 publications

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“…The results obtained when the antiserum to the 72-kd protein (the viral E2 gene product) was used clearly show that early gene expression could be detected in the high-multiplicity hr-1 infection (Fig. 4B); thus, there must also have been expression of the 12S EtA RNA in these cells, as has been shown previously (3,37). Therefore, the ElA peptide serum detects only the 13S products which were absent from the hr-1 infection.…”

supporting

confidence: 67%

“…The use of appropriate viral systems affords the opportunity to overcome these limitations. The expression of adenovirus genes during the early phase of a viral infection is strictly regulated at multiple levels of RNA biogenesis (33,37,50). Much of the regulation of early viral gene expression occurs at the level of transcription and is mediated through the action of specific viral gene products (32,34,35).…”

mentioning

confidence: 99%

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Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells.

Feldman¹,

Nevins²

1983

Mol. Cell. Biol.

102

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The function of the adenovirus E1Aa protein (the product of the 13S ElA mRNA) during a productive viral infection is to activate transcription of the six early viral transcription units. To study the mechanism of action of this protein, a peptide which was 13 amino acids long and had a sequence unique to the protein product of the adenovirus 13S ElA mRNA (pElAa) was coupled to keyhole limpet hemocyanin and used to raise an antibody in rabbits. The resulting antiserum was specific to this protein and did not react with the protein product of the 12S ElA mRNA, which shares considerable sequence with the E1Aa protein.This antiserum was used to probe for the E1Aa protein in situ by indirect immunofluorescence and in extracts of infected HeLa cells. We found that the protein was associated with large cellular structures both in the nucleus and in the cytoplasm. The nuclear form of the protein was analyzed further and was found to purify with the nuclear matrix.

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supporting

confidence: 67%

mentioning

confidence: 99%

Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells.

Feldman¹,

Nevins²

1983

Mol. Cell. Biol.

102

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“…The human adenovirus (adeno), type 2 genome has seven transcription units that are expressed early after infection, the L1, Ela, Elb, E2a, E2b, E3, and E4 regions (1). Of these the E3 region codes for a Mr 19,000 protein (2).…”

mentioning

confidence: 99%

Homology of adenoviral E3 glycoprotein with HLA-DR heavy chain.

Chatterjee¹,

Maizel²

1984

Proc. Natl. Acad. Sci. U.S.A.

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The Mr 19,000 adenovirus glycoproteins coded by the E3 region that is expressed on the cell membrane and presumably binds to HLA class I antigen shows sequence homology to the a-chain of human HLA-DR and to the -chain of human IgM. The homology extends to a conserved domain present in al of the major histocompatibility complex antigens, P2-microglobulins, and immungobulins. Predicted ,& sheet secondary structures are similar between the Mr 19,000 adenovirus protein and these immune system proteins. Evolutionary and functional implications of the homology are discussed.

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“…However, as the E2 and E3 transcription units do not hybridize a human probe, there must be something particular about E1A, E1B, and E4, which do allow hybridization. The only major difference in the transcriptional regulation between El A, E1B, and E4 on the one hand and E2 and E3 on the other, is the change in promoter sites utilized between early and intermediate expression (Chow et ai, 1979) for the E2 and E3 genes (see Persson and Philipson, 1982, for a review). Once the exact sequences involved in the hybridization of Ad2 and human DNA have been identified the differences between these two classes of genes might be clearer.…”

Section: Discussionmentioning

confidence: 99%

Adenoviruses Have Homology with a Reiterated Sequence in Genomic DNA

Braithwaite¹,

Lejeune²,

Naora³

1984

DNA

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The DNAs of human adenovirus types 2 and 5 (Ad2, Ad5) hybridize to genomic DNAs from human and chicken tissue. The pattern of Southern blot hybridization to genomic DNA digested with Eco RI suggests that sequences homologous to cloned Ad2 DNA are reiterated in genomic DNA. This was confirmed by liquid hybridization which shows that the hybrids have a low C0t 1/2 value. Finally, we show that total genomic human DNA probes hybridize to short fragments of adenoviral DNA in early-region genes near the ends of the viral genome.

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Regulation of Adenovirus Gene Expression

Cited by 20 publications

References 245 publications

Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells.

Localization of the adenovirus E1Aa protein, a positive-acting transcriptional factor, in infected cells infected cells.

Homology of adenoviral E3 glycoprotein with HLA-DR heavy chain.

Adenoviruses Have Homology with a Reiterated Sequence in Genomic DNA

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