Homology of adenoviral E3 glycoprotein with HLA-DR heavy chain.

Abstract

The Mr 19,000 adenovirus glycoproteins coded by the E3 region that is expressed on the cell membrane and presumably binds to HLA class I antigen shows sequence homology to the a-chain of human HLA-DR and to the -chain of human IgM. The homology extends to a conserved domain present in al of the major histocompatibility complex antigens, P2-microglobulins, and immungobulins. Predicted ,& sheet secondary structures are similar between the Mr 19,000 adenovirus protein and these immune system proteins. Evolutionary and functional implications of the homology are discussed.

The Endoplasmic Reticulum Lumenal Domain of the Adenovirus Type 2 E3-19K Protein Binds to Peptide-Filled and Peptide-Deficient HLA-A*1101 Molecules

“…1A). Two Asn residues (Asn 12 and Asn 61 ) have been identified as putative N glycosylation sites in the amino acid sequence of E3-19K (44). To firmly establish that the three bands observed on the SDS-PAGE gel correlates with the glycosylated nature of E3-19K, a sample of purified E3-19K was incubated with PNGase F under denaturing conditions (Fig.…”

The recognition event between virus and host cell receptor: a target for antiviral agents

“…Class I HLA MHC molecule (Chatterjee & Maizel, 1984) Class I HLA MHC molecule via flz-microglobulin (Grundy et al, …”

“…Residues 1-141 of E3gp (Chatterjee & Maizel, 1984) Residues 204-297 of 1-1301 ordomain gene product (Beck & Barrell, 1988) Residues 21--29, 372-378 of gp350 (Nemerow et al, …”

“…Residues 95 105 of VPI capsid protein (Murray et al, 1988) Class II HLA-DR MHC molecule or-chain and IgM x-chain (Chatterjee & Maizel, 1984) Class I MHC molecule ~z3 chain (Beck & Barrell, 1988) C3 complement fragment C3d (Nemerow et aL, 1987;Tanner et al, …”

Cell-type-specific synthesis of murine immunoglobulin mu RNA from an adenovirus vector.

“…heavy chains of class I transplantation antigen (64) and shares structural homology with HLA-DR heavy chain and ,B2-microglobulin (17). This mutant has a second deletion between map units 3.8 and 10 and therefore lacks part of the ElA and all of the E1B transcription units, making it necessary to propagate the virus on a permissive cell line such as 293, which complements adenovirus El defects (35).…”