Regulation of 70-Kilodalton Heat-Shock-Like Messenger Ribonucleic Acid<i>in Vitro</i>and<i>in Vivo</i>by Prolactin

deToledo, Sonia M.; Murphy, Liam; Hatton, T.; Friesen, Henry G.

doi:10.1210/mend-1-6-430

Cited by 41 publications

(5 citation statements)

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“…For example, Nb2 cells display an abnormal response to heat shock. Indeed, whereas the hsp70 -like mRNA is upregulated following lactogen stimulation [ 41 ], no expression of the inducible hsp70 (GenBank X74271) gene was detected. As components of the heat-shock response are involved in normal cell-cycle-progression, the abnormalities observed in Nb2 cells may have important consequences for their growth.…”

Section: Resultsmentioning

confidence: 99%

Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Bôle‐Feysot

Perret²,

Roustan³

et al. 2000

Genome Biol

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Background: Rat Nb2-11C lymphoma cells are dependent on prolactin for proliferation and are widely used to study prolactin signaling pathways. To investigate the role of this hormone in the transcriptional mechanisms that underlie prolactin-stimulated mitogenesis, five different techniques were used to isolate differentially expressed transcripts: mRNA differential display, representational difference analysis (RDA), subtractive suppressive hybridization (SSH), analysis of weakly expressed candidate genes, and differential screening of an organized library.

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Section: Resultsmentioning

confidence: 99%

Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Bôle‐Feysot

Perret²,

Roustan³

et al. 2000

Genome Biol

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“…To identify genes that may play a role in Prl-mediated growth control, the strategy was to isolate genes that are expressed early after Prl stimulation of quiescent Nb2 T cells without protein synthesis. Incubation with Prl for 4 h was chosen because previous studies have determined that two growthrelated genes, c-myc (13,47) and Nb29, a heat shock protein 70 homolog (8,47), were induced 5-to 10-fold at the transcriptional level at 4 h after Prl stimulation (47). A lambda ZAP Nb2 T-cell cDNA library was differentially screened with first-strand cDNAs prepared from quiescent versus Prl-plus-CHX-stimulated cells.…”

Section: Resultsmentioning

confidence: 99%

“…To reinitiate growth, 10 ng of Prl (NIH-P-S13 from the National Institute of Diabetes and Digestive and Kidney Diseases) per ml was added. Protein synthesis inhibitors were used at either 10 ,ug of CHX per ml (10 mg/ml stock in water) or 30 ,uM anisomycin (8,OOOx stock in ethanol).…”

Section: Methodsmentioning

confidence: 99%

Interferon-regulatory factor 1 is an immediate-early gene under transcriptional regulation by prolactin in Nb2 T cells.

Yu-Lee¹,

Hrachovy²,

Stevens³

et al. 1990

Mol. Cell. Biol.

144

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The pituitary peptide hormone prolactin (Prl) is a potent inducer of Nb2 T lymphoma cell proliferation. To analyze the early genetic response to the mitogenic signals of Pri, a cDNA library was constructed from Nb2 T cells stimulated for 4 h with Prl and the protein synthesis inhibitor cycloheximide. Of 26 distinct clones isolated by differential screening, one clone, designated c25, exhibited extremely rapid but transient kinetics of induction by Prl and superinduction by Prl plus cycloheximide. Run-on transcription analysis indicated that c25 gene transcription was induced greater than 20-fold within 30 to 60 min of Prl stimulation. Surprisingly, DNA sequence analysis of c25 cDNA revealed that this Prl-inducible early-response gene is the rat homolog of the mouse transcription factor interferon-regulatory factor 1 (IRF-1), sharing 91% coding sequence similarity with mouse IRF-1. At the protein level, rat IRF-1 shares 97% and 92% homology with mouse IRF-1 and human IRF-1, respectively, suggesting that this molecule has been functionally conserved throughout evolution. Our studies show that the gene for IRF-1 is an immediate-early gene in Prl-stimulated T cells, which suggests that IRF-1 is a multifunctional molecule. In addition to its role in regulating growth-inhibitory interferon genes, IRF-1 may, therefore, also play a stimulatory role in cell proliferation. The gene for IRF-1 is one of the earliest genes known to be transcriptionally regulated by Prl.

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“…Kiefer et al 1991). Filters were also hybridized with pNb29, a cDNA encoding a constitutively expressed rat heat shock¬ like protein as a control for RNA loading (deToledo et al 1987). Autoradiography was performed by exposing Kodak X-Omat AR film (Eastman Kodak, Rochester, NY, U.S.A.) to the nitrocellulose filters at -70°C in the presence of an enhancing screen for up to 6 days.…”

Section: Rna Extraction and Northern Blot Analysismentioning

confidence: 99%

Effects of oestrogen on rat uterine expression of insulin-like growth factor-binding proteins

Molnar

Murphy²

1994

Journal of Molecular Endocrinology

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Previous studies have established that the IGFs are involved in oestrogen-induced uterine proliferation. IGF-binding proteins (IGFBPs) are present in most biological fluids and tissues and may modulate the actions of the IGFs. We examined uterine, hepatic and renal expression of the IGFBPs throughout the oestrous cycle and investigated the effects of oestradiol (OE2) on IGFBP expression in ovariectomized (ovx) rats. Uterine expression of IGFBPs-1 and -3 showed a definite variation throughout the oestrous cycle with highest levels during dioestrus. In the liver and kidney the changes in IGFBP-1 and IGFBP-3 mRNA abundance were the opposite of those observed in the uterus, with the highest levels observed during oestrus. Administration of OE2 to ovx rats decreased uterine IGFBP-3 mRNA and increased IGFBP-4 mRNA levels. In these rats there were no consistent changes in renal IGFBP-1 or IGFBP-3 mRNAs; however, a significant increase in IGFBP-4 mRNA was observed in this tissue, as in the uterus. In the liver an increase in IGFBP-1 mRNA and a decrease in IGFBP-3 mRNA levels were observed in rats treated with OE2. Despite changes in uterine, hepatic and renal IGFBP mRNA levels, no significant variation was seen in serum IGFBPs as determined by ligand blotting of sera. These data demonstrate that there is a cyclical variation in the expression of the IGFBPs in the uterus, kidney and liver, and that OE2 is able to modulate differentially IGFBP expression in these tissues.

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Regulation of 70-Kilodalton Heat-Shock-Like Messenger Ribonucleic Acidin Vitroandin Vivoby Prolactin

Cited by 41 publications

References 0 publications

Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Interferon-regulatory factor 1 is an immediate-early gene under transcriptional regulation by prolactin in Nb2 T cells.

Effects of oestrogen on rat uterine expression of insulin-like growth factor-binding proteins

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