Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Bôle‐Feysot, Christine; Perret, Eric; Roustan, P.; Bouchard, Brigitte; Kelly, Paul A.

doi:10.1186/gb-2000-1-4-research0008

Cited by 27 publications

(9 citation statements)

References 82 publications

(87 reference statements)

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“…These data collectively support the proposal that Egr-1 acts as a growth stimulator for B lymphoma cells and that reduction of Egr-1 levels (antisense ODN) or function (dominant-negative approach) leads to growth inhibition. Consistent with our data, Egr-1 has been found to play an important role in many transformed cells, including human prostate cancer (12), Nb2 lymphoma (49), and body cavity BC-2 lymphoma (13) cells.…”

Section: Discussionsupporting

confidence: 90%

The Role of MAPKs in B Cell Receptor-induced Down-regulation of Egr-1 in Immature B Lymphoma Cells

Gururajan

Kumar

et al. 2006

Journal of Biological Chemistry

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The immediate-early gene egr-1 (also known as NGFI-A, krox24, zif268, and tis8) encodes a transcription factor containing three tandem zinc finger motifs that bind to GC-rich DNA elements in the promoters of a range of target genes to activate their transcription (1). egr-1 expression is elicited in response to a diverse variety of signals, including growth factors, cytokines, lipopolysaccharide, serum, irradiation, stress, hypoxia, and urea, in many cell types, including neuronal cells, epithelial cells, fibroblasts, myeloid cells, and T and B lymphocytes (2-9). In many studies, Egr-1 was found to be associated with cell proliferation, differentiation, and transformation (3, 10 -14). Egr-1 was also shown to induce apoptosis in certain cell types in response to irradiation by activating p53 (15) or PTEN expression (16). Yu et al. (17) reported that differential post-translational modification of Egr-1 (acetylation versus phosphorylation) is likely responsible for its different roles in promoting growth versus apoptosis in response to serum and UV irradiation. Moreover, Egr-1 is shown to be important for production of pro-inflammatory cytokines such as interleukin-1␤, interleukin-13, and tumor necrosis factor as well as chemokines (18 -20).In normal mature B lymphocytes, signaling through the B cell receptor (BCR) 2 induces rapid and transient expression of egr-1 (6), but its importance for subsequent B cell activation and proliferation is unknown. In contrast, BCR engagement in immature B lymphoma cells fails to induce egr-1 expression, and the lymphoma cells undergo growth inhibition and apoptosis (21). Thus, BCR-induced positive versus negative signals are reflected in the differential expression of egr-1. Interestingly, an immature B lymphoma cell line (BKS-2) constitutively expresses high levels of Egr-1, and antisense oligodeoxynucleotides (ODNs) to Egr-1 inhibit BKS-2 cell growth (14). BCR signaling, which causes growth arrest and apoptosis of BKS-2 cells, also down-regulates egr-1 (14). The down-regulation of Egr-1 and growth inhibition caused by anti-IgM antibody are reversed by CpG ODN (22). These data suggest a positive correlation between the levels of Egr-1 and growth of B lymphoma cells. An examination of the microarray data published by Alizadeh et al. (23) revealed that egr-1 expression is elevated in ϳ35% of human diffuse large B lymphoma cells, supporting the notion that Egr-1 is important for B lymphoma cell growth.Several studies in different cell lines have shown that the rapid induction of Egr-1 with various stimuli is mediated * This work was supported by National Institutes of Health Grant 5P01CA092372 (to S. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C.

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Section: Discussionsupporting

confidence: 90%

The Role of MAPKs in B Cell Receptor-induced Down-regulation of Egr-1 in Immature B Lymphoma Cells

Gururajan

Kumar

et al. 2006

Journal of Biological Chemistry

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“…In addition to elevated serum PRL levels, male PRLtg mice also have elevated testosterone levels. The reason for this is that PRL stimulates LH-receptor expression in the testicular Leydig cells of rodents, resulting in increased testosterone production [13,18]. When the level of serum testosterone was normalized or eliminated in PRL-tg males, resistin mRNA expression was unchanged compared to control mice.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies indicate that prolactin (PRL) is a diabetogenic hormone and hyperprolactinemia induces glucose intolerance, hyperinsulinaemia and insulin resistance in both human patients and rodents [5^12]. The PRL receptor (PRLR) is a member of the cytokine receptor superfamily and it mediates the e¡ects of PRL on its target tissues [13]. In a recent study, we demonstrated PRLR expression in mouse adipocytes [14].…”

Section: Introductionmentioning

confidence: 99%

Increased resistin expression in the adipose tissue of male prolactin transgenic mice and in male mice with elevated androgen levels

et al. 2001

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The aim of this study was to investigate the regulation of resistin, a recently identified adipocyte-secreted peptide, in the adipose tissue of prolactin (PRL)-transgenic (tg) mice using ribonuclease protection assay. The level of resistin mRNA increased 3.5-fold in the adipose tissue of untreated male PRLtg mice compared to controls. However, there was no difference in resistin expression in the adipose tissue of female PRL-tg mice compared to control mice. PRL-tg male mice have elevated serum testosterone levels and we therefore analyzed the effects of testosterone alone on resistin mRNA expression. Furthermore, the effects of elevated androgen levels on PRL receptor (PRLR) mRNA expression in the adipose tissue were investigated. Resistin mRNA increased 2.6-fold in the adipose tissue of control male mice with elevated serum androgen levels. In addition, PRLR mRNA expression was increased in the adipose tissue of male mice with elevated testosterone. These results suggest testosterone to be a regulator of resistin and PRLR mRNA expression in the adipose tissue of male mice. ß

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“…The level of PRL-R mRNA strongly increases during late pregnancy. PRL has multiple functions throughout the body including growth and di¡erentiation and immune response [1] and it remains di¤cult to assign a precise role for uterine PRL. It will therefore be of great interest to further investigate a possible role for PRL and/or related hormones acting through the PRL-R in the establishment of an adequate uterine environment allowing the maintenance of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…Prolactin (PRL) is a pleitropic hormone involved in a large number of physiological actions that can be divided into the following categories: water and electrolyte balance, growth and development, endocrinology and metabolism, brain and behavior, reproduction and immunoregulation and protection [1]. The e¡ects of PRL are mediated through an interaction with a speci¢c high a¤nity cell surface receptor.…”

Section: Introductionmentioning

confidence: 99%

Cellular localization and evolution of prolactin receptor mRNA in ovine endometrium during pregnancy

et al. 1999

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In this study, we have investigated the expression of the prolactin receptor gene in ovine endometrium during oestrus cycle and pregnancy. Using reverse transcription-PCR analysis, we provided evidence that the prolactin receptor gene is specifically transcribed in this tissue. As shown by Northern blot analysis, the level of the prolactin receptor transcripts increased dramatically during late pregnancy. In situ hybridization experiments revealed that prolactin receptor mRNA was specifically expressed in the glandular compartment and confirmed the dramatic increase of its expression that occurs at the end of pregnancy. Taken together, these findings are consistent with a putative role of prolactin and/or related molecules in the regulation of the proliferation of the glandular compartment and/or in the control of the secretory activity of the endometrium.z 1999 Federation of European Biochemical Societies.

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Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Cited by 27 publications

References 82 publications

The Role of MAPKs in B Cell Receptor-induced Down-regulation of Egr-1 in Immature B Lymphoma Cells

The Role of MAPKs in B Cell Receptor-induced Down-regulation of Egr-1 in Immature B Lymphoma Cells

Increased resistin expression in the adipose tissue of male prolactin transgenic mice and in male mice with elevated androgen levels

Cellular localization and evolution of prolactin receptor mRNA in ovine endometrium during pregnancy

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