Cellular localization and evolution of prolactin receptor mRNA in ovine endometrium during pregnancy

Cassy, Sandrine; Charlier, Madia; Guillomot, Michel; Pessemesse, Laurence; Djiane, Jean

doi:10.1016/s0014-5793(99)00052-6

Cited by 20 publications

(13 citation statements)

References 33 publications

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“…Ovine PL in vitro can heterodimerize the extracellular domains of oGHR and oPRLR, and if this heterodimerization were to occur in a living cell it could activate signal transduction [28]. In the ovine uterus, oPRLR expression is abundant and restricted to the endometrial GE [6,27], whereas oGHR expression is relatively much lower and detectable by reverse transcription PCR in most uterine cell types, including the endometrial GE [26] (unpublished results). In the present study, SEAP-oPL binding to endometrial GE was completely displaced by oPL and oPRL, whereas oGH only partly displaced binding of SEAP-oPL to the endometrial GE.…”

Section: Discussionmentioning

confidence: 90%

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Ovine Placental Lactogen Specifically Binds to Endometrial Glands of the Ovine Uterus1

Noel

Herman

Johnson

et al. 2003

Biology of Reproduction

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A hormonal servomechanism has been proposed to regulate differentiation and function of the endometrial glandular epithelium (GE) in the ovine uterus during pregnancy. This mechanism involves sequential actions of estrogen, progesterone, ovine interferon tau (IFNtau), placental lactogen (oPL), and placental growth hormone (oGH). The biological actions of oPL in vitro are mediated by homodimerization of the prolactin receptor (oPRLR) and heterodimerization of the oPRLR and oGH receptor. The objectives of the study were to determine the effects of intrauterine oPL, oGH, and their combination on endometrial histoarchitecture and gene expression and to localize and characterize binding sites for oPL in the ovine uterus in vivo using an in situ ligand binding assay. Intrauterine infusion of oPL and/or oGH following IFNtau into ovariectomized ewes treated with progesterone daily differentially affected endometrial gland number and expression of uterine milk proteins and osteopontin. However, neither hormone affected PRLR, insulin-like growth factor (IGF)-I, or IGF-II mRNA levels in the endometrium. A chimeric protein of placental secretory alkaline phosphatase (SEAP) and oPL was used to identify and characterize binding sites for oPL in frozen sections of interplacentomal endometrium from pregnant ewes. Specific binding of SEAP-oPL was detected in the endometrial GE on Days 30, 60, 90, and 120 of pregnancy. In Day 90 endometrium, SEAP-oPL binding to the endometrial GE was displaced completely by oPL and prolactin (oPRL) but only partially by oGH. Binding experiments using the extracellular domain of the oPRLR also showed that iodinated oPL binding sites could be competed for by oPRL and oPL but not by oGH. Collectively, results indicate that oPL binds to receptors in the endometrial glands and that oPRL is more effective than oGH in competing for these binding sites. Thus, effects of oPL on the endometrial glands may be mediated by receptors for oPRL and oGH.

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Section: Discussionmentioning

confidence: 90%

“…In the sheep uterus, oPRL receptor (oPRLR) expression is specifically restricted to the GE and increases during pregnancy [6,27]. This receptor transduces signals by oPRL and oPL because oPL homodimerizes the oPRLR and activates signaling pathways [28].…”

Section: Introductionmentioning

confidence: 99%

Ovine Placental Lactogen Specifically Binds to Endometrial Glands of the Ovine Uterus1

Noel

Herman

Johnson

et al. 2003

Biology of Reproduction

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“…The ratio has been shown to vary with growth activity in other ovine and bovine (Schuler et al 1997) tissues. For example, long form expression of the ewe endometrium was elevated during the second half of pregnancy (Cassy et al 1999). Such changes in isoform ratio presumably regulate the magnitude and type of signalling, either by competitive inhibition of the full length receptor (Das & Vonderhaar 1995) or activation of different signalling pathways (Bignon et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Regulation of prolactin receptor expression in ovine skin in relation to circulating prolactin and wool follicle growth status

Nixon

Ca²,

Wildermoth³

et al. 2002

Journal of Endocrinology

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Seasonal patterns of hair growth are governed, at least in part, by levels of prolactin in circulation, and although receptors for prolactin (PRLR) have been demonstrated in hair follicles, little is known of their regulation in relation to follicular cycles. In this study, a photoperiod-generated increase in prolactin was used to induce a wool follicle cycle during which changes in PRLR expression in sheep skin were determined by ribonuclease protection assay and in situ hybridisation. mRNA for prolactin and both isoforms of PRLR were also detected in skin by reverse transcription and polymerase chain reaction. As circulating prolactin began to rise from low levels, PRLR mRNA in the skin initially fell. These changes immediately preceded the catagen (regressive) phase of the hair cycle. Further increase in prolactin resulted in up-regulation of PRLR during telogen (dormancy), particularly in the epithelial hair germ, to reach a peak during proanagen (reactivation). In anagen (when follicle growth was fully re-established), PRLR mRNA returned to levels similar to those observed before the induced cycle. Hence, this longer term rise and fall of PRLR expression followed that of plasma prolactin concentration with a lag of 12-14 days. PRLR mRNA was most abundant in the dermal papilla, outer root sheath, hair germ, skin glands and epidermis. Location of PRLR in the dermal papilla and outer root sheath indicates action of prolactin on the growth-controlling centres within wool follicles. These cycle-related patterns of PRLR expression suggest dynamic regulation of PRLR by prolactin, thereby modulating hormonal responsiveness of seasonally growing hair follicles.

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“…A homodimer of the PRL receptor (PRLR), as well as a heterodimer of PRLR and GH receptor, transduce signals by ovine CSH1/placental lactogen (Gertler & Djiane 2002). In the ovine uterus, PRLR gene expression is unique to GE (Cassy et al 1999, Stewart et al 2000.…”

Section: Csh1 Regulation Of Uterine Gland Morphogenesis and Secretorymentioning

confidence: 99%

Genes involved in conceptus–endometrial interactions in ruminants: insights from reductionism and thoughts on holistic approaches

Spencer¹,

Sandra²,

Wolf³

2008

Reproduction

272

253

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This review summarizes new knowledge on expression of genes and provides insights into approaches for study of conceptus-endometrial interactions in ruminants with emphasis on the peri-implantation stage of pregnancy. Conceptus-endometrial interactions in ruminants are complex and involve carefully orchestrated temporal and spatial alterations in gene expression regulated by hormones from the ovary and conceptus. Progesterone is the hormone of pregnancy and acts on the uterus to stimulate blastocyst survival, growth, and development. Inadequate progesterone levels or a delayed rise in progesterone is associated with pregnancy loss. The mononuclear trophectoderm cells of the elongating blastocyst synthesize and secrete interferon-t (IFNT), the pregnancy recognition signal. Trophoblast giant binucleate cells begin to differentiate and produce hormones including chorionic somatomammotropin 1 (CSH1 or placental lactogen). A number of genes, induced or stimulated by progesterone, IFNT, and/or CSH1 in a cell-specific manner, are implicated in trophectoderm adhesion to the endometrial luminal epithelium and regulation of conceptus growth and differentiation. Transcriptional profiling experiments are beginning to unravel the complex dynamics of conceptus-endometrial interactions in cattle and sheep. Future experiments should incorporate physiological models of pregnancy loss and be complemented by metabolomic studies of uterine lumen contents to more completely define factors required for blastocyst survival, growth, and implantation. Both reduction and holistic approaches will be important to understand the multifactorial phenomenon of recurrent pregnancy loss and provide a basis for new strategies to improve pregnancy outcome and reproductive efficiency in cattle and other domestic animals.

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Cellular localization and evolution of prolactin receptor mRNA in ovine endometrium during pregnancy

Cited by 20 publications

References 33 publications

Ovine Placental Lactogen Specifically Binds to Endometrial Glands of the Ovine Uterus1

Ovine Placental Lactogen Specifically Binds to Endometrial Glands of the Ovine Uterus1

Regulation of prolactin receptor expression in ovine skin in relation to circulating prolactin and wool follicle growth status

Genes involved in conceptus–endometrial interactions in ruminants: insights from reductionism and thoughts on holistic approaches

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