Regulation mechanism of aquaporin 9 gene on inflammatory response and cardiac function in rats with myocardial infarction through extracellular signal-regulated kinase1/2 pathway

Huang, Xingmei; Yu, Xiaobin; Li, Hongxia; Han, Lu; Yang, Xiangjun

doi:10.1007/s00380-019-01452-8

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…it has been demonstrated that aQP9 is notably upregulated in patients with heart failure and Mi (46,61). in addition, silencing of the aQP9 gene was revealed to inhibit apoptosis of myocardial cells and improve cardiac function following Mi (62). Thus, it has been demonstrated that hsa-mir-24-1-5p and aQP9 may play a significant role in cardiac function following MI.…”

Section: Discussionmentioning

confidence: 97%

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Wang¹,

Cao²

2020

Mol Med Rep

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Myocardial infarction (Mi) is one of the leading causes of death globally. The aim of the present study was to find valuable micrornas (mirnas/mirs) and target mrnas in order to contribute to our understanding of the pathology of Mi. mirna and mrna data were downloaded for differential expression analysis. Then, a regulatory network between mirnas and mrnas was established, followed by function annotation of target mrnas. Thirdly, prognosis and diagnostic analysis of differentially methylated target mrnas were performed. Finally, an in vitro experiment was used to validate the expression of selected mirnas and target mrnas. a total of 19 differentially expressed mirnas and 1,007 differentially expressed mRNAs were identified. Several regulatory interaction pairs between mirna and mrnas were identified, such as hsa-mir-142-2p-long-chain-fattyacid-coa ligase 1 (acSl1), hsa-mir-15a-3p-nicotinamide phosphoribosyltransferase (naMPT), hsa-mir-33b-5p-regulator of G-protein signaling 2 (rGS2), hsa-mir-17-3p-Jun dimerization protein 2 (JdP2), hsa-mir-24-1-5p-aquaporin-9 (aQP9) and hsa-mir-34a-5p-STaT1/aKT3. of note, it was demonstrated that acSl1, naMPT, rGS2, JdP2, aQP9, STaT1 and aKT3 had diagnostic and prognostic values for patients with Mi. in addition, STaT1 was involved in the 'chemokine signaling pathway' and 'Jak-STaT signaling pathway'. aKT3 was involved in both the 'MaPK signaling pathway' and 'T cell receptor signaling pathway'. reverse transcription-quantitative Pcr validation of hsa-mir-142-3p, hsa-mir-15a-3p, hsa-mir-33b-5p, acSl1, naMPT, rGS2 and JdP2 expression was consistent with the bioinformatics analysis. In conclusion, the identified miRNAs and mRNAs may be involved in the pathology of Mi.

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Section: Discussionmentioning

confidence: 97%

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Wang¹,

Cao²

2020

Mol Med Rep

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“…For example, AQP9 in the peripheral blood could discriminate patients with CD from patients with chronic inflammation and healthy controls (23). In rat myocardial infarction model, the mRNA and protein expression levels of AQP9 was significantly increased, and silencing AQP9 gene can inhibit the activation of ERK1/2 signaling pathway, attenuate the inflammatory response in rats with myocardial infarction, inhibit apoptosis of myocardial cells, and improve cardiac function (24). In addition, AQP9 was showed to play a role in regulating tissuespecific physiological properties in tight junctions in ulcerative colitis (25).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and Predictive Value of Immune-Related Genes in Crohn’s Disease

Yin

Tang

et al. 2021

Front. Immunol.

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Abnormal immune cell infiltration is associated with the pathogenesis of Crohn’s disease (CD). This study aimed to determine the diagnostic and predictive value of immune-related genes in CD. Seven Gene Expression Omnibus datasets that analyzed the gene expression in CD tissues were downloaded. Single-sample gene set enrichment analysis (ssGSEA) was used to estimate the infiltration of the immune cells in CD tissues. Immune-related genes were screened by overlapping the immune-related genes with differentially expressed genes (DEGs). The protein-protein interaction (PPI) network was used to identify key immune-related DEGs. Diagnostic value of CD and predictive value of anti-TNFα therapy were analyzed. Immunohistochemical (IHC) assay was used to verify gene expression in CD tissues. There were significant differences among CD tissues, paired CD tissues, and normal intestinal tissues regarding the infiltration of immune cells. AQP9, CD27, and HVCN1 were identified as the key genes of the three sub-clusters in the PPI network. AQP9, CD27, and HVCN1 had mild to moderate diagnostic value in CD, and the diagnostic value of AQP9 was better than that of CD27 and HVCN1. AQP9 expression was decreased in CD after patients underwent anti-TNFα therapy, but no obvious changes were observed in non-responders. AQP9 had a moderate predictive value in patients who had undergone treatment. IHC assay confirmed that the expression of AQP9, CD27, and HVCN1 in CD tissues was higher than that in normal intestinal tissues, and AQP9, CD27 was correlated with the activity of CD. Immune-related genes, AQP9, CD27, and HVCN1 may act as auxiliary diagnostic indicators for CD, and AQP9 could serve as a promising predictive indicator in patients who underwent anti-TNF therapy.

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“… 30 Huang et al found that silencing of the AQP9 gene improved cardiac function following MI. 31 C5AR1 is involved in complement and coagulation cascades in AMI. 32 PLAUR, a conserved gene, is up-regulated in AMI.…”

Section: Discussionmentioning

confidence: 99%

Screening for Regulatory Network of miRNA–Inflammation, Oxidative Stress and Prognosis-Related mRNA in Acute Myocardial Infarction: An in silico and Validation Study

Yin¹,

Wang²,

Wang³

et al. 2022

IJGM

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Background Acute myocardial infarction (AMI), which commonly leads to heart failure, is among the leading causes of mortality worldwide. The aim of this study was to find potential regulatory network for miRNA-inflammation, oxidative stress and prognosis-related mRNA to uncover molecular mechanisms of AMI. Methods The expression profiles of miRNA and mRNA in the blood samples from AMI patients were downloaded from the Gene Expression Omnibus (GEO) dataset for differential expression analysis. Weighted gene co-expression network analysis (WGCNA) was used to further identify important mRNAs. The negatively regulatory network construction of miRNA–inflammation, oxidative stress and prognosis-related mRNAs was performed, followed by protein–protein interaction (PPI) and functional analysis of mRNAs. Results A total of three pairs of negatively regulatory network of miRNA–inflammation and prognosis-related mRNAs (hsa-miR-636/hsa-miR-491-3p/hsa-miR-188-5p/hsa-miR-188-3p-AQP9, hsa-miR-518a-3p-C5AR1 and hsa-miR-509-3-5p/hsa-miR-127-5p-PLAUR), two pairs of negatively regulatory network of miRNA–oxidative stress and prognosis-related mRNAs (hsa-miR-604-TLR4 and hsa-miR-139-5p-CXCL1) and three pairs of negatively regulatory network of miRNA-inflammation, oxidative stress and prognosis-related mRNA (hsa-miR-634/hsa-miR-591-TLR2, hsa-miR-938-NFKBIA and hsa-miR-520h/hsa-miR-450b-3p-ADM) were identified. In the KEGG analysis, some signaling pathways were identified, such as complement and coagulation cascades, pathogenic Escherichia coli infection, chemokine signaling pathway and cytokine–cytokine receptor interaction and Toll-like receptor signaling pathway. Conclusion Identified negatively regulatory network of miRNA-inflammation/oxidative stress and prognosis-related mRNA may be involved in the process of AMI. Those inflammation/oxidative stress and prognosis-related mRNAs may be diagnostic and prognostic biomarkers for AMI.

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Regulation mechanism of aquaporin 9 gene on inflammatory response and cardiac function in rats with myocardial infarction through extracellular signal-regulated kinase1/2 pathway

Cited by 13 publications

References 29 publications

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Uncovering potential differentially expressed miRNAs and targeted mRNAs in myocardial infarction based on integrating analysis

Diagnostic and Predictive Value of Immune-Related Genes in Crohn’s Disease

Screening for Regulatory Network of miRNA–Inflammation, Oxidative Stress and Prognosis-Related mRNA in Acute Myocardial Infarction: An in silico and Validation Study

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