Regulation and Exploitation of Enzyme Biosynthesis

Enzymes of the Genus Aspergillus

“…Dissolved oxygen concentration is measured throughout, but fermenter design may limit gaseous availability. Factors that affect production of microbial enzymes in submerged fermentation and regulation of enzyme synthesis have been reviewed by a number of workers (Aunstrup, 1977;Frost and Moss, 1987;Demain, 1990). …”

Media for Industrial Fermentations

“…However, the cost may prohibit their use as inducers in a commercial process. Reviews have been published by Aunstrup et al (1979) and Demain (1990).…”

The Isolation, Preservation and Improvement of Industrially Important Micro-organisms

“…For example, the E. coli ßgalactosidase constitutive mutants isolated by Horiuchi et al (1963) in chemostat culture also contained up to four copies of the lac Ζ gene. According to Demain (1990), during the 1960s and early 1970s the number of gene copies were increased by using plasmids or trans-ducing phage in the same species. The production of /3-galactosidase, penicillinase, chloramphenicol transacetylase and aspartate transcarbamylase were all increased by transferring plasmids containing the structural gene into recipient cultures, especially when the plasmid replicated faster than the host chromosome.…”