Regulating apoptosis in mammalian cell cultures

Arden, Nilou; Betenbaugh, Michael J.

doi:10.1007/s10616-006-9008-5

Cited by 25 publications

(19 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The market for monoclonal antibodies (mAb) alone is expected to grow 30% a year [1]. This demand has provided a challenge for developing efficient and cost-effective processes for mAb production.…”

Section: Introductionmentioning

confidence: 99%

Effect of α-Ketoglutarate on Monoclonal Antibody Production of Hybridoma Cell Lines in Serum-Free and Serum-Containing Medium

Nilsang

Kumar

Rakshit

2008

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Process development and optimization for increase population growth and protein productivity in mammalian cell culture have been studied for many years. In this study, the behavior of hybridoma cells was investigated using six-well micro-titer plate systems with a working volume of 4 ml. Mouse hybridoma cell lines D2 and 2C83G2 were seeded in serum-free and serum-containing media and cultured for 8 days. alpha-Ketoglutarate is an integral component of the tricarboxylic acid (TCA) cycle and is produced from glutamine via glutamate. To study its effect on cell growth, metabolism, and monoclonal antibody (mAb) production, 2 mM alpha-ketoglutarate (pH 7.2) was added in both media at the beginning of the cultivation and in another set after 72 h. High cell density was observed in D2 cell culturing in serum-free medium, while 2C83G2 cell line showed high cell density in serum-containing medium. However, both cell lines cultured in serum-free medium gave viability above 70% when grown for 8 days. The supplement of 2 mM alpha-ketoglutarate supported cell growth and mAb production of both hybridoma cell lines in serum-free and serum-containing medium. The addition of alpha-ketoglutarate at the beginning of the batch cultivation gave better result in cell growth and mAb production as compared to alpha-ketoglutarate supplementation after 72 h. However, addition after 72 h was better than no addition at all. This indicates that alpha-ketoglutarate have a positive effect on production and release of antibody.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of α-Ketoglutarate on Monoclonal Antibody Production of Hybridoma Cell Lines in Serum-Free and Serum-Containing Medium

Nilsang

Kumar

Rakshit

2008

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

“…However, the productivity of mammalian cells is low as compared with that of prokaryotic hosts, which increases the cost of recombinant protein production and purification. Strategies have been developed in order to increase recombinant protein production in CHO cells, such as the regulation of culture temperature (Fox et al 2004), the addition of chemicals (Kim et al 2005;Rodriguez et al 2005), bioreactor engineering (Nienow 2006), and inhibition of cell apoptosis (Arden and Betenbaugh 2006). For example, Fox et al (2004) used a biphasic temperature process to maximize the recombinant protein, i.e.…”

Section: Introductionmentioning

confidence: 99%

Enhanced recombinant M-CSF production in CHO cells by glycerol addition: model and validation

Liu

Chen

2007

Cytotechnology

View full text Add to dashboard Cite

Addition of stimulatory chemical such as glycerol was found to increase recombinant protein production in Chinese hamster ovary (CHO) cells. However, glycerol influenced cell mitosis and reduced cell growth rate. We developed a controlled proliferation strategy to utilize the stimulation of glycerol on recombinant protein production and mitigate the problem of growth inhibition. The approach is to apply a two-stage process, where cells are cultured without glycerol for a period of time in order to obtain enough cell density and then glycerol is added to achieve high specific productivity. In addition, a model for predicting the profiles of cell proliferation and recombinant protein production was developed and validated. A two-stage process, addition of 1% glycerol after 1 day of growth, could increase the final production of macrophage-colony stimulating factor (M-CSF) by 38% compared with the value obtained without addition of glycerol.

show abstract

“…Apoptosis works on the two basic pathways called extrinsic and intrinsic; when there is a stress factor in a cell, apoptosis starts. When apoptosis starts, the cell digests itself via activation of pro-apoptotic genes in the Bcl2 gene family [2]. Unlike apoptosis, necrosis is a caspase-independent type of cell death.…”

Section: Autophagy Mechanismmentioning

confidence: 99%

The Role of Autophagy in Stress and Cancer

Bostancıklıoğlu¹

2015

Single Cell Biol

View full text Add to dashboard Cite

Programmed cell death plays an important role in development and disease. There are three main types of programmed cell death: apoptosis, autophagy, and necrosis. Apoptosis works on the two basic pathways called extrinsic and intrinsic; when there is a stress factor in a cell, apoptosis starts. Autophagy is critical for the continuation of normal human physiology such as the cellular homeostasis, energy balance, development and cellular defense. In addition to these, it may play a role in the pathogenesis of cancer, neurodegenerative diseases, aging, muscular diseases, infectious diseases, and immune system diseases.While some autophagy genes showed tumor suppressive effect during the development of cancer, some other genes contributes to the survival of cancer cells during cancer progression. Therefore, the relationship between autophagy and cancer is quite complex. The role of autophagy in cancer varies depending on the stage of the cancer, the metabolic status of cell and the presence of stress. Also, it was reported that the expression of certain molecules associated with autophagy vary in different types of cancer. Autophagy is activated and allows the continuation of the viability of tumor cells in hypoxia. In case of nutrient deficiency, autophagy allows tumor tissue to gain time until nutrient, oxygen and growth factors become eligible again. Therefore, autophagy is seen as a necessary mechanism for tumor continuity. Autophagy increases both in cancer cells and normal cells during the cancer treatment. But, due to cancer cells use autophagy to survive more than normal cells; this case may generate new therapeutic opportunities. The studies needed to conduct to find new ways for using the genes play role in autophagy in cancer treatment.In the present study, we aimed to throw light on the interaction of autophagy mechanism and some stresses such as ischemia and reperfusion and cancer development.

show abstract

Regulating apoptosis in mammalian cell cultures

Cited by 25 publications

References 94 publications

Effect of α-Ketoglutarate on Monoclonal Antibody Production of Hybridoma Cell Lines in Serum-Free and Serum-Containing Medium

Effect of α-Ketoglutarate on Monoclonal Antibody Production of Hybridoma Cell Lines in Serum-Free and Serum-Containing Medium

Enhanced recombinant M-CSF production in CHO cells by glycerol addition: model and validation

The Role of Autophagy in Stress and Cancer

Contact Info

Product

Resources

About