Registration of GPP5BR(M/H/F)C3 Anthracnose‐Resistant Sorghum Germplasm Population

Duncan, R. R.; Hepperly, P. R.; Torres-Cardona, S.; Sotomayor-Ríos, A.

doi:10.2135/cropsci1991.0011183x003100010075x

Cited by 4 publications

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“…A biparental mapping population was generated from an initial cross of the parents ‘Bk7’ (female parent; made male-sterile by tying a plastic bag over the flower at the onset of anther dehiscence), and ‘Early Hegari-Sart’ (EH-S). ‘Bk7’ is a grain sorghum generated by Dr. Daniel Gorbet (University of Florida, retired) based on an initial selection made from the genetically diverse GPP5BR germplasm population of Sorghum bicolor (L.) Moench ( Duncan et al 1991 ). The pure line ‘Bk7’ resulted from inbreeding the initial selection for nine generations while selecting for resistance to several diseases prevalent in North Florida, most notably anthracnose, and short stature to enable combine harvesting of the seed.…”

Section: Methodsmentioning

confidence: 99%

Using Genotyping by Sequencing to Map Two Novel Anthracnose Resistance Loci inSorghum bicolor

Felderhoff

McIntyre

Saballos

et al. 2016

G3 Genes|Genomes|Genetics

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Colletotrichum sublineola is an aggressive fungal pathogen that causes anthracnose in sorghum [Sorghum bicolor (L.) Moench]. The obvious symptoms of anthracnose are leaf blight and stem rot. Sorghum, the fifth most widely grown cereal crop in the world, can be highly susceptible to the disease, most notably in hot and humid environments. In the southeastern United States the acreage of sorghum has been increasing steadily in recent years, spurred by growing interest in producing biofuels, bio-based products, and animal feed. Resistance to anthracnose is, therefore, of paramount importance for successful sorghum production in this region. To identify anthracnose resistance loci present in the highly resistant cultivar ‘Bk7’, a biparental mapping population of F3:4 and F4:5 sorghum lines was generated by crossing ‘Bk7’ with the susceptible inbred ‘Early Hegari-Sart’. Lines were phenotyped in three environments and in two different years following natural infection. The population was genotyped by sequencing. Following a stringent custom filtering protocol, totals of 5186 and 2759 informative SNP markers were identified in the two populations. Segregation data and association analysis identified resistance loci on chromosomes 7 and 9, with the resistance alleles derived from ‘Bk7’. Both loci contain multiple classes of defense-related genes based on sequence similarity and gene ontologies. Genetic analysis following an independent selection experiment of lines derived from a cross between ‘Bk7’ and sweet sorghum ‘Mer81-4’ narrowed the resistance locus on chromosome 9 substantially, validating this QTL. As observed in other species, sorghum appears to have regions of clustered resistance genes. Further characterization of these regions will facilitate the development of novel germplasm with resistance to anthracnose and other diseases.

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Section: Methodsmentioning

confidence: 99%

Using Genotyping by Sequencing to Map Two Novel Anthracnose Resistance Loci inSorghum bicolor

Felderhoff

McIntyre

Saballos

et al. 2016

G3 Genes|Genomes|Genetics

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“…Early Hegari-Sart is an experimental forage sorghum line (Pedersen et al, 1982;Oliver et al, 2005). Bk7 is a grain sorghum cultivar derived from the GPP5BR germplasm population (Duncan et al, 1991), following nine generations of selection by Dr. Daniel Gorbet (University of Florida, retired) for resistance to several diseases prevalent in Florida. In a preliminary screening of adult plants under water-limited field conditions, Bk7 was identified as drought-sensitive on the basis of the observation that its leaves were wilted and partially desiccated, whereas the EH plants were unaffected (Fig.…”

Section: Plant Materials and Experimental Design For Phenotypingmentioning

confidence: 99%

QTLs Associated with Crown Root Angle, Stomatal Conductance, and Maturity in Sorghum

et al. 2017

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Three factors that directly affect the water inputs in cropping systems are root architecture, length of the growing season, and stomatal conductance to water vapor (g s ). Deeper-rooted cultivars will perform better under water-limited conditions because they can access water stored deeper in the soil profile. Reduced g s limits transpiration rate (E) and thus throughout the vegetative phase conserves water that may be used during grain filling in water-limited environments. Additionally, growing early-maturing varieties in regions that rely on soil-stored water is a key water management strategy. To further our understanding of the genetic basis underlying root depth, growing season length, and g s we conducted a quantitative trait locus (QTL) study. A QTL for crown root angle (a proxy for root depth) new to sorghum was identified in chromosome 3. For g s , a QTL in chromosome seven was identified. In a follow-up field study it was determined that the QTL for g s was associated with reduced E but not with net carbon assimilation rate (A) or shoot biomass. No differences in guard-cell length or stomatal density were observed among the lines, leading to the conclusion that the observed differences in g s must be explained by partial stomatal closure. The well-studied maturity gene Ma1 was identified in the QTL for maturity. The transgressive segregation of the population was explained by the possible interaction of Ma1 with other loci. Finally, the most probable position of the genes underlying the QTLs and candidate genes were proposed.

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A cost-benefit analysis to select the most effective method for positional cloning: genotyping by sequencing versus allele-specific PCR

2017

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Registration of GPP5BR(M/H/F)C3 Anthracnose‐Resistant Sorghum Germplasm Population

Cited by 4 publications

References 0 publications

Using Genotyping by Sequencing to Map Two Novel Anthracnose Resistance Loci inSorghum bicolor

Using Genotyping by Sequencing to Map Two Novel Anthracnose Resistance Loci inSorghum bicolor

QTLs Associated with Crown Root Angle, Stomatal Conductance, and Maturity in Sorghum

A cost-benefit analysis to select the most effective method for positional cloning: genotyping by sequencing versus allele-specific PCR

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