Registration of Five Leaf Spot‐Resistant Peanut Germplasm Lines

Stalker, H. T.; Beute, M. K.; Shew, B. B.; Isleib, T. G.

doi:10.2135/cropsci2002.3140

Cited by 38 publications

(18 citation statements)

References 2 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides these resistance sources from cultivated peanut, wild peanut relative A. cardenasii was found to harbor strong resistance to both ELS and LLS (Abdou et al, 1974). Introgression of A. cardenasii chromatin accomplished through a synthetic hexaploid bridge has led to the release of multiple ELS resistant lines including the male parent of our mapping population GP-NC WS 16 (Stalker and Beute, 1993;Stalker et al, 2002;Tallury et al, 2014). The same resistance source was sent to ICRISAT, India in the 1980s and used in breeding programs resulting in the release of GPBD 4 and ICGV86699 (Reddy et al, 1996;Stalker, 2017).…”

Section: Introductionmentioning

confidence: 99%

Major QTLs for resistance to early and late leaf spot diseases are identified on chromosomes 3 and 5 in peanut (Arachis hypogaea)

Chu

Chee

Culbreath

et al. 2019

Preprint

View full text Add to dashboard Cite

Early and late leaf spots are the major foliar diseases of peanut responsible for severely decreased yield in the absence of intensive fungicide spray programs. Pyramiding host resistance to leaf spots in elite cultivars is a sustainable solution to mitigate the diseases. In order to determine the genetic control of leaf spot disease resistance in peanut, a recombinant inbred line population (Florida-07 x GP-NC WS16) segregating for resistance to both diseases was used to construct a SNP-based linkage map consisting of 855 loci. QTL mapping revealed three resistance QTLs for late leaf spot qLLSA05 (phenotypic variation explained, PVE=7-10%), qLLSB03 (PVE=5-7%), and qLLSB05 (PVE=15-41%) that were consistently expressed over multi-year analysis. Two QTL, qLLSA05 and qLLSB05, confirmed our previously published QTL-seq results. For early leaf spot, three resistance QTLs were identified in multiple years, two on chromosome A03 (PVE=8-12%) and one on chromosome B03 (PVE=13-20%), with the locus qELSA03_1.1 coinciding with the previously published genomic region for LLS resistance in GPBD4. Comparative analysis of the genomic regions spanning the QTLs suggests that resistance to early and late leaf spots are largely genetically independent. In addition, QTL analysis on yield showed that the presence of resistance allele in qLLSB03 and qLLSB05 loci might result in protection from yield loss caused by LLS disease damage. Finally, post hoc analysis of the RIL subpopulation that was not utilized in the QTL mapping revealed that the flanking markers for these QTLs can successfully select for resistant and susceptible lines, confirming the effectiveness of pyramiding these resistance loci to improve host-plant resistance in peanut breeding programs using marker-assisted selection.

show abstract

Section: Introductionmentioning

confidence: 99%

Major QTLs for resistance to early and late leaf spot diseases are identified on chromosomes 3 and 5 in peanut (Arachis hypogaea)

Chu

Chee

Culbreath

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, the improvement of existing cultivars and/or development of new cultivars with greater levels of field resistance is the most effective economical means of controlling these diseases and is a major objective in peanut breeding programs [4]. Although peanut cultivars and breeding lines with greater resistance to TSWV or leaf spots have been developed and released [4, 5], variations in disease resistance among these cultivars do exist. To supplement this incomplete resistance, single or multiple genes with known metabolic pathway(s) can be engineered into existing peanut cultivars to increase the effectiveness of natural disease resistance.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Gene Expression Profiles in Leaf Tissues of Cultivated Peanuts and Development of EST‐SSR Markers and Gene Discovery

Guo

Chen

Hong

et al. 2009

International Journal of Plant Genomics

View full text Add to dashboard Cite

Peanut is vulnerable to a range of foliar diseases such as spotted wilt caused by Tomato spotted wilt virus (TSWV), early (Cercospora arachidicola) and late (Cercosporidium personatum) leaf spots, southern stem rot (Sclerotium rolfsii), and sclerotinia blight (Sclerotinia minor). In this study, we report the generation of 17,376 peanut expressed sequence tags (ESTs) from leaf tissues of a peanut cultivar (Tifrunner, resistant to TSWV and leaf spots) and a breeding line (GT-C20, susceptible to TSWV and leaf spots). After trimming vector and discarding low quality sequences, a total of 14,432 high-quality ESTs were selected for further analysis and deposition to GenBank. Sequence clustering resulted in 6,888 unique ESTs composed of 1,703 tentative consensus (TCs) sequences and 5185 singletons. A large number of ESTs (5717) representing genes of unknown functions were also identified. Among the unique sequences, there were 856 EST-SSRs identified. A total of 290 new EST-based SSR markers were developed and examined for amplification and polymorphism in cultivated peanut and wild species. Resequencing information of selected amplified alleles revealed that allelic diversity could be attributed mainly to differences in repeat type and length in the SSR regions. In addition, a few additional INDEL mutations and substitutions were observed in the regions flanking the microsatellite regions. In addition, some defense-related transcripts were also identified, such as putative oxalate oxidase (EU024476) and NBS-LRR domains. EST data in this study have provided a new source of information for gene discovery and development of SSR markers in cultivated peanut. A total of 16931 ESTs have been deposited to the NCBI GenBank database with accession numbers ES751523 to ES768453.

show abstract

“…In order to obtain tetraploid fertile offspring of the diploid wild type and the allotetraploid cultivated peanut, complicated breeding programs have to be designed. It normally takes many years to get a hybrid with agronomical value (Simpson et al 1993;Stalker and Beute, 1993;Simpson and Starr, 2001). Marker assisted selection could increase the efficiency and predictability during the process of wild gene pool utilization.…”

mentioning

confidence: 99%

EST sequencing and SSR marker development from cultivated peanut ( Arachis hypogaea L.)

Song¹,

Li²,

Han³

et al. 2010

Electron. J. Biotechnol.

View full text Add to dashboard Cite

Registration of Five Leaf Spot‐Resistant Peanut Germplasm Lines

Cited by 38 publications

References 2 publications

Major QTLs for resistance to early and late leaf spot diseases are identified on chromosomes 3 and 5 in peanut (Arachis hypogaea)

Major QTLs for resistance to early and late leaf spot diseases are identified on chromosomes 3 and 5 in peanut (Arachis hypogaea)

Analysis of Gene Expression Profiles in Leaf Tissues of Cultivated Peanuts and Development of EST‐SSR Markers and Gene Discovery

EST sequencing and SSR marker development from cultivated peanut ( Arachis hypogaea L.)

Contact Info

Product

Resources

About