Registration of Common Bacterial Blight, Rust and Bean Common Mosaic Resistant Great Northern Common Bean Germplasm Line ABC‐Weihing

Abstract

Great northern common bean (Phaseolus vulgaris L.) germplasm line ABC‐Weihing (Reg. No. GP‐246, PI 647964) was developed by the University of Nebraska Agricultural Research Division in cooperation with USDA‐ARS and released in 2006. This line, tested as NE1‐05‐4, was bred specifically for enhanced resistance to common bacterial blight (CBB), a major seed borne disease of common bean caused by the bacterium Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp). ABC‐Weihing is a great northern BC5F3:6 line obtained from five backcrosses (‘Weihing’*5//‘Chase’/XAN 159). The first cross was made in spring 1997. Only BCnF1 plants resistant to Xcp isolates Dominican Republic DR‐7 and Nebraska SC4A, as determined by multiple needle leaf inoculation tests in the greenhouse, were used for successive backcrossing. In addition to phenotypic selection for CBB resistance, marker‐assisted selection for the resistant QTL‐linked marker SU91 was conducted in the BC1F1, BC2F1, and ABC‐Weihing. When inoculated with Nebraska Xcp strains in the field, ABC‐Weihing exhibited resistance in both 2005 and 2006. ABC‐Weihing has Ur‐3 and Ur‐6 genes for resistance to common bean rust and carries the single dominant hypersensitive I gene that provides resistance to all non‐necrotic strains of the Bean common mosaic virus (BCMV). ABC‐Weihing has bright white seed, blooms 45 d after planting, and is a midseason bean maturing 92 d after planting.

Common Bacterial Blight Resistance QTL BC420 and SU91 Effect on Seed Yield, Seed Weight, and Canning Quality in Dry Bean

“…Although O'Boyle et al (2007) observed a slight yield penalty of 7% in one of two environments for black bean lines developed with the SU91 marker, many high‐yielding lines with SU91 were also observed. The ABC Weihing great northern bean (Mutlu et al, 2008) with SU91 derived by five backcrosses had similar yield to the recurrent parent Weihing (Coyne et al, 2000), 5177 versus 5107 kg ha −1 , in a recent CBB‐free field trial (unpublished) that we conducted in Othello, WA, in 2015. Mutlu et al (2005b) observed that pinto bean ABCP‐8 with SU91 developed from backcrossing had higher yield than the recurrent parent ‘Chase’ pinto in nondiseased trials (Coyne et al, 1994).…”

“…The identification of major quantitative trait loci (QTL) controlling resistance to CBB (reviewed by Miklas et al, 2006a; Miklas and Singh, 2007; Viteri et al, 2014b; Singh and Miklas, 2015) has facilitated marker‐assisted breeding for higher levels of CBB resistance into better‐adapted and higher‐yielding dry bean lines (Miklas et al, 2000; Mutlu et al, 2005a). The QTL and linked markers, ultimately derived from great northern landrace and tepary bean, have been used to develop dry bean germplasm lines USDK‐CBB‐15 (Miklas et al, 2006b), USWK‐CBB‐17 (Miklas et al, 2006c), ABCP‐8 (Mutlu et al, 2005b), USCR‐CBB‐20 (Miklas et al, 2011), and cultivars such as ‘ABC‐Weihing’ (Mutlu et al, 2008). Generally, the markers tightly linked with the QTL have been used for marker‐assisted backcrossing and early‐generation selection of the resistance genes, which is then followed up by traditional phenotypic selection using pathogen challenge in the greenhouse and field during later generations (reviewed by Viteri et al, 2014b; Singh and Miklas, 2015).…”

Achievements and limitations of contemporary common bean breeding using conventional and molecular approaches

“…Low soil fertility tolerance Derived from germplasm accessions, landraces and improved lines Singh et al (2003a, b) Low soil fertility and drought tolerance Beebe et al (2008) Multiple disease and pest resistance Angular leaf spot, anthracnose, Common bacterial blight, bean Common mosaic virus, Bean golden mosaic virus and leafhopper resistance Singh et al (1998) Common bacterial blight, rust and Bean common mosaic virus resistance Mutlu et al (2005Mutlu et al ( , 2008 Pyramided rust, Bean common mosaic virus and Bean common necrotic mosaic resistance genes producing high levels of resistance Pastor-Corrales (2003) and PastorCorrales et al (2007) Multiple virus resistance Scully et al (1995) Bean common mosaic virus, Beet curly top virus and root rot resistance Silbernagel et al (1998) Smith et al (2007 many important bean diseases have been identified and used in cultivar development programs (Miklas et al 2006b;Beaver et al 2003). However, breeders also need information from plant pathologists concerning the virulence patterns of the pathogens in order to most effectively deploy disease resistance genes .…”

The Sustainability of Agro-Food and Natural Resource Systems in the Mediterranean Basin

“…The objective of the present work was to evaluate the response to CBB of five traditional bean varieties (Tondino bianco, Verdolino, Cannellino, Tabacchino and Ciuoto) of the FS variety pool, in comparison to four available resistant bean breeding lines (VAX-4, USDK-CBB-15, ABC-Wiehing and USCR-CBB-20) (Singh et al 2001;Miklas et al 2006Miklas et al , 2011Mutlu et al 2008). Aliquots of bacterial suspensions of the highly virulent Xap and Xapf strains were inoculated into the first trifoliate mesophyll of bean plants (Lo Cantore et al 2010a).…”