Regional variation in brain capillary density and vascular response to ischemia

Cavaglià, Marco; Dombrowski, Stephen; Drazba, Judith A.; Vasanji, Amit; Bokesch, Paula M.; Janigro, Damir

doi:10.1016/s0006-8993(01)02637-3

Cited by 237 publications

(198 citation statements)

References 43 publications

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“…The variation of MVD and VSI among three cerebral regions found in the healthy volunteers matched very well with general anatomical observations (Cavaglia et al, 2001), i.e., the diameter of microvessels in WM and GM is very similar and lower vessel density is observed in WM than in GM.…”

Section: Feasibility Of Vessel Size Imaging In Acute Strokesupporting

confidence: 85%

Vessel Size Imaging Reveals Pathological Changes of Microvessel Density and Size in Acute Ischemia

Schmidt

Villringer

et al. 2011

J Cereb Blood Flow Metab

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The aim of this study was to test the feasibility of vessel size imaging with precise evaluation of apparent diffusion coefficient and cerebral blood volume and to apply this novel technique in acute stroke patients within a pilot group to observe the microvascular responses in acute ischemic tissue. Microvessel density-related quantity Q and mean vessel size index (VSI) were assessed in 9 healthy volunteers and 13 acute stroke patients with vessel occlusion within 6 hours after symptom onset. Our results in healthy volunteers matched with general anatomical observations. Given the limitation of a small patient cohort, the median VSI in the ischemic area was higher than that in the mirrored region in the contralateral hemisphere (P < 0.05). Decreased Q was observed in the ischemic region in 2 patients, whereas no obvious changes of Q were found in the remaining 11 patients. In a patient without recanalization, the VSI hyperintensity in the subcortical area matched well with the final infarct. These data reveal that different observations of microvascular response in the acute ischemic tissue seem to emerge and vessel size imaging may provide useful information for the definition of ischemic penumbra and have an impact on future therapeutic approaches.

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Section: Feasibility Of Vessel Size Imaging In Acute Strokesupporting

confidence: 85%

Vessel Size Imaging Reveals Pathological Changes of Microvessel Density and Size in Acute Ischemia

Schmidt

Villringer

et al. 2011

J Cereb Blood Flow Metab

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“…In bromocriptine-and saline-treated animals, EBA-immunoreactive vessels were evenly distributed in all structures on both sides with densities that reflected regional differences in vascularization (cf. saline control, Figure 2u-x; Cavaglia et al, 2001). By contrast, L-DOPA-treated animals showed areas lacking EBAimmunoreactive vessels on the DA-depleted side (Table 1; Figure 2m-p; one-factor ANOVA, p GP ¼ 0.018, p EP ¼ 0.010, p SNr ¼ 0.001).…”

Section: Angiogenesis Is Induced By L-dopa But Not Bromocriptinementioning

confidence: 90%

Differential Involvement of D1 and D2 Dopamine Receptors in L-DOPA-Induced Angiogenic Activity in a Rat Model of Parkinson's Disease

Lindgren

Ohlin

Cenci

2009

Neuropsychopharmacol

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Angiogenesis occurs in the brains of Parkinson's disease patients, but the effects of dopamine replacement therapy on this process have not been examined. Using rats with 6-hydroxydopamine lesions, we have compared angiogenic responses induced in the basal ganglia by chronic treatment with either L-DOPA, or bromocriptine, or a selective D1 receptor agonist (SKF38393). Moreover, we have asked whether L-DOPA-induced angiogenesis can be blocked by co-treatment with either a D1-or a D2 receptor antagonist (SCH23390 and eticlopride, respectively), or by an inhibitor of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (SL327). L-DOPA, but not bromocriptine, induced dyskinesia, which was associated with endothelial proliferation, upregulation of immature endothelial markers (nestin) and downregulation of endothelial barrier antigen in the striatum and its output structures. At a dose inducing dyskinesia (1.5 mg/kg/day), SKF38393 elicited angiogenic changes similar to L-DOPA. Antagonism of D1-but not D2 class receptors completely suppressed both the development of dyskinesia and the upregulation of angiogenesis markers. In fact, L-DOPA-induced endothelial proliferation was markedly exacerbated by low-dose D2 antagonism (0.01 mg/kg eticlopride). Inhibition of ERK1/2 by SL327 attenuated L-DOPA-induced dyskinesia and completely inhibited all markers of angiogenesis. These results highlight the specific link between treatment-induced dyskinesias and microvascular remodeling in the dopamine-denervated brain. L-DOPA-induced angiogenesis requires stimulation of D1 receptors and activation of ERK1/2, whereas the stimulation of D2 receptors seems to oppose this response.

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“…Since previous studies showed destruction of the blood-brain barrier (BBB) several hours after injection of Optison into the carotid artery 14 or insonation after intravenous injection of Optison, 15 we carefully evaluated the safety aspect of the present gene transfer method. We first evaluated the change in body weight, tissue damage by hematoxylin and eosin (HE) staining, and destruction of the BBB by microangiography using albumin-fluorescein isothiocyanate (FITCalbumin), 16 as the microbubble-enhanced ultrasound Figure 4 Local gene expression produced by plasmid DNA transfer using microbubble-enhanced ultrasound as assessed by luciferase expression. Luciferase activity was measured in the brainstem and cerebellum, left hemisphere, right hemisphere, and olfactory bulb at 1 day after transfection.…”

Section: Safety Evaluationmentioning

confidence: 99%

“…To evaluate BBB function, we used a recently developed microangiographic technique. 16 This technique allows evaluation of BBB function as well as vascular pattern. Briefly, fluorescent albumin solution was prepared by reconstituting 500 mg bovine desiccate albumin-fluorescein isothiocyanate (SigmaAldrich, USA) in 50 ml PBS.…”

Section: Histological Stainingmentioning

confidence: 99%

Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound

et al. 2004

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Regional variation in brain capillary density and vascular response to ischemia

Cited by 237 publications

References 43 publications

Vessel Size Imaging Reveals Pathological Changes of Microvessel Density and Size in Acute Ischemia

Vessel Size Imaging Reveals Pathological Changes of Microvessel Density and Size in Acute Ischemia

Differential Involvement of D1 and D2 Dopamine Receptors in L-DOPA-Induced Angiogenic Activity in a Rat Model of Parkinson's Disease

Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound

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