Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound

Shimamura, Munehisa; Sato, Naoyuki; Taniyama, Yoshiaki; Yamamoto, Seiji; Endoh, Masayuki; Kurinami, Hitomi; Aoki, Motokuni; Ogihara, Toshio; Kaneda, Yasufumi; Morishita, Ryuichi

doi:10.1038/sj.gt.3302323

Cited by 99 publications

(70 citation statements)

References 30 publications

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“…To overcome this problem, we previously developed an efficient gene transfer method into the adult rat brain based on a microbubble-enhanced ultrasound method using plasmid DNA. 13 In a previous study, we transfected foreign genes by intracisternal or intrastriatal injection. 13 In this study, we modified the previous method to obtain a more effective method.…”

Section: Ultrasound-mediated Gene Transfer Into Cerebral Ventriclementioning

confidence: 99%

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Alleviation of Aβ-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model

et al. 2008

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A new therapeutic approach to treat Alzheimer's disease (AD) is needed, and the use of growth factors is considered to be a candidate. Hepatocyte growth factor (HGF) is a unique multifunctional growth factor, which has the potential effect to exert neurotrophic action and induce angiogenesis. In this study, we examined the effects of overexpression of human HGF plasmid DNA using ultrasound-mediated gene transfer into the brain in an Ab-infused cognitive dysfunction mouse model. We demonstrated that HGF gene transfer significantly alleviated Ab-induced cognitive impairment in mice in behavioral tests. These beneficial effects of HGF might be due to (1) significant recovery of the vessel density in the dentate gyrus of the hippocampus, (2) upregulation of BDNF, (3) a significant decrease in oxidative stress and (4) synaptic enhancement. A pharmacological approach including gene therapy to increase the HGF level in combination with anti-Ab therapy might be a new therapeutic option for the treatment of AD.

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Section: Ultrasound-mediated Gene Transfer Into Cerebral Ventriclementioning

confidence: 99%

“…13 In a previous study, we transfected foreign genes by intracisternal or intrastriatal injection. 13 In this study, we modified the previous method to obtain a more effective method. Initially, we examined gene expression of a marker gene, luciferase.…”

Section: Ultrasound-mediated Gene Transfer Into Cerebral Ventriclementioning

confidence: 99%

Alleviation of Aβ-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model

et al. 2008

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“…(Current address: Department of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Oaska, 565-0871, Japan. Phone: +81-6-6879-3652: FAX: +81-6-6879-3659; e-mail : m-kinoshita@nsurg.med.osaka-u.ac.jp) hyperthermia, such as coagulation of tissues [1][2][3][4][5][6][7][8][9][10][11][12], mechnical tissue destruction [13,14], disruption of tight junctions of the vasculature for enhanced local drug delivery [15][16][17][18][19][20], and sonoporation, a transient enhanced permeability of the cell membrane caused by the combination of ultrasound and microbubbles [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. As all of these bio-effects can be created non-invasively in vivo, ultrasound is gathering a great attention as a next generation surgical or drug delivery system.…”

Section: Introductionmentioning

confidence: 99%

Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Kinoshita

Hynynen

2007

Biochemical and Biophysical Research Communications

104

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Ultrasound induced intracellular drug delivery, sonoporation, is an appealing and promising technique for next generation drug delivery system. Many types of molecules, such as plasmid DNAs, siRNAs and peptides, have been demonstrated to be delivered into the cell by ultrasound with the aid of microbubbles both in vitro and in vivo. Although there are many reports on in vitro sonoporation, the efficiency of successful sonoporation and the viabilities of cells after the procedure documented in each report vary in a wide range, and the reasons for these differences are not fully understood. In this study, we have investigated how different experimental settings would affect sonoporation efficiency and cell viabilities after the procedure. Our results show that the fashion of cell culture (e.g. in suspension or in monolayer culture) and the presence of standing wave have a great impact on the overall results. These results indicate that in vitro sonoporation settings should be carefully evaluated in each experiment. The fact that standing wave is necessary to achieve high sonoporation efficiency may be a problematic issue for clinical application of sonoporation, as it may be difficult (although not impossible) to create standing wave in a human body.

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“…Gene delivery efficiency appears to be controlled by pulse intensity, frequency and duration. [139] Sonoporationmediated gene delivery has been demonstrated in the cornea, [140] brain, [141] CNS, [142][143][144] bone, [145] peritoneal cavity, [146] kidney, [147] pancreas, [148] liver, [149,150] embryonic tissue, [151] dental pulp, [152] muscle [153,154] and heart. [155,156] More recent studies in mouse liver showed that inclusion of gas-filled microbubbles enhanced gene delivery efficiency.…”

Section: Sonoporationmentioning

confidence: 99%

Advances in Gene Delivery Systems

et al. 2011

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The transfer of genes into cells, both in vitro and in vivo, is critical for studying gene function and conducting gene therapy. Methods that utilize viral and nonviral vectors, as well as physical approaches, have been explored. Viral vector-mediated gene transfer employs replication-deficient viruses such as retro-virus, adenovirus, adeno-associated virus and herpes simplex virus. A major advantage of viral vectors is their high gene delivery efficiency. The nonviral vectors developed so far include cationic liposomes, cationic polymers, synthetic peptides and naturally occurring compounds. These nonviral vectors appear to be highly effective in gene delivery to cultured cells in vitro but are significantly less effective in vivo. Physical methods utilize mechanical pressure, electric shock or hydrodynamic force to transiently permeate the cell membrane to transfer DNA into target cells. They are simpler than viral-and nonviral-based systems and highly effective for localized gene delivery. The past decade has seen significant efforts to establish the most desirable method for safe, effective and target-specific gene delivery, and good progress has been made. The objectives of this review are to (i) explain the rationale for the design of viral, nonviral and physical methods for gene delivery; (ii) provide a summary on recent advances in gene transfer technology; (iii) discuss advantages and disadvantages of each of the most commonly used gene delivery methods; and (iv) provide future perspectives.

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Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound

Cited by 99 publications

References 30 publications

Alleviation of Aβ-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model

Alleviation of Aβ-induced cognitive impairment by ultrasound-mediated gene transfer of HGF in a mouse model

Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Advances in Gene Delivery Systems

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