Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Mapping Method

Abstract

Postoperative abnormal neurobehavioral performance (PANP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to PANP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as the potential association with PANP is still not well understood. Here, a battery of behavioral tests was used to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and the metabolites in 12 different sub-regions of the brain at different time points were investigated by proton nuclear magnetic resonance (1 H-NMR) spectroscopic technique. The abnormal neurobehavioral performance occurred at 6h and/or 9h, and recovered at 24h after anesthesia/surgery. Compared with the control group, the altered metabolites of the model group at 6h were aspartate (Asp) and N-acetyl-aspartate (NAA), and these differences were mainly displayed in the cortex; while significant changes at 9h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu), these changes returned to baseline level at 24h. The altered metabolic changes could have occurred as a result of the acute PANP, and metabolites of Asp and Glu in the cortex and hippocampus could provide preliminary evidence to our understanding of the PANP process.

Regional cerebral metabolic levels and turnover in awake rats after acute or chronic spinal cord injury

“…The metabolic information, required to determine metabolic fluxes, commonly vary with cerebral regions as shown in the human, 44 rat, 45 and mouse 46 brains. Both the cerebral cortex and the deep brain regions have their own variation characteristics after SCI.…”

Neurochemical alterations of different cerebral regions in rats with myocardial ischemia-reperfusion injury based on proton nuclear magnetic spectroscopy analysis

“…To minimize the impact of post-mortem changes on cerebral metabolites [ 28 ], the anesthetized rat was microwaved using our previous method [ 29 ]. After euthanasia, the rat brain was quickly removed according to the Allen Brain Atlas [ 30 ] and dissected into 12 different regions (as indicated in Figure 8 ) [ 31 ]. Briefly, the olfactory bulb (OB) and the cerebellum (CE) were first sampled, followed by the hippocampus (HP), the thalamus (THA) and the striatum (STR).…”

“…Protocols for cerebral tissue extraction, PMRS spectrum acquirement and PMRS data processing were the same as those described in our previous study [ 29 , 30 , 32 ]. Detailed methods are displayed in the Supplementary Materials .…”

“…Although the underlying neurometabolic coupling mechanisms are still not completely understood, PMRS has already become a valued tool to detect amino acid levels and thus, brain-wide metabolic networks mapping. In the current study, we used PMRS to examine the alterations of amino acid levels in different brain regions associated with myocardial IR injury reproduced by the intermittent occlusion of the left anterior descending coronary artery [9,[24][25][26][27][28][29][30][31][32].…”

Specific Patterns of Spinal Metabolite Ratio Underlying α-Me-5-HT-evoked Pruritus Compared with Compound 48/80 Based on Proton Nuclear Magnetic Resonance Spectroscopy

“…Recently, our laboratory has used the proton nuclear MRS to investigate specific patterns of spinal metabolites underlying histamine-independent pruritogen α-Me-5-HT-evoked pruritus compared with histamine and capsaicin [7] . Results from these studies provide a general strategy for the previously proposed method [9][10][11][12] . Proton nuclear MRS is a commonly used tool to measure levels of N-acetylaspartate (NAA), glutamate (Glu), creatine (Cr), myo-inositol (Ins), etc.…”