Regional cerebral metabolic levels and turnover in awake rats after acute or chronic spinal cord injury

Wu, Liang; Niu, Zhanfeng; Hu, Xulei; Liu, Huili; Li, Shuang; Chen, Lei; Zheng, Danhao; Liu, Zhuang; Liu, Taotao; Xu, Fuqiang; Manyande, Anne; Wang, Jie; Xia, Hechun

doi:10.1096/fj.202000447r

Cited by 12 publications

(7 citation statements)

References 55 publications

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“…The NMR spectra for all samples were collected randomly at 298 K using a BrukerAvance III 500 MHz NMR spectrometer from BrukerBiospin in Germany. The data were generated using the POCE ([ 1 H- 13 C]-NMR) pulse sequence, a widely adopted method for detecting 13 C enrichment in cerebral extractions [22]. This approach involves two spin-echo detections: one without a broad reverse pulse applied at the 13 C frequency (the total metabolite concentrations, 12 C+ 13 C), and another with a reverse pulse (the difference of metabolites, 12 C-13 C).…”

Section: Nmr Spectrum Acquisitionmentioning

confidence: 99%

Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique

Chen,

Wu,

et al. 2024

Mol Neurobiol

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Lactate is not only the energy substrate of neural cells, but also an important signal molecule in brain. In modern societies, disturbed circadian rhythms pose a global challenge. Therefore, exploring the in uence of circadian period on lactate and its metabolic kinetics is essential for the advancement of neuroscienti c research. In the present study, mice were infused [3-13 C] lactate through the tail vein for a duration of two minutes. After 30 minutes lactate metabolism, the animals were sacri ced and the brain tissue was extracted and tested. And then the [ 1 H-13 C] NMR technology was employed to investigate the kinetic information of lactate metabolism in different brain regions during various time periods and acute sleep deprivation, to detect the enrichment of various neurochemicals. The results revealed uctuating lactate concentrations in the brain throughout the day, with lower levels during light periods and higher levels during dark periods.Most metabolites displayed strong sensitivity to circadian rhythm, exhibiting signi cant day-night variations.Conversely, only a few metabolites showed changes after acute sleep deprivation, primarily in the temporal brain region. Interestingly, in contrast to brain lactate metabolism, liver lactate metabolism exhibited a signi cant increase following acute sleep deprivation. This study explored the kinetics of lactate metabolism, hinted at potential clinical implications for disorders involving circadian rhythm disturbances, and providing a new research basis for clinical exploration of brain lactate metabolism.

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Section: Nmr Spectrum Acquisitionmentioning

confidence: 99%

Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique

Chen,

Wu,

et al. 2024

Mol Neurobiol

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“…According to our previous study [36], before the experiment, all animals were fasted overnight and only had free access to water (16-18 hours) in order to reduce endogenous glucose levels. On the following experimental day, the rats were anesthetized with 2.0% isoflurane, and a PE50 tube (Intech, PA, USA) was catheterized into the tail vein for the infusion of [1-13 C] glucose (Qingdao Tenglong Weibo Technology Co., LTD, Qingdao, P.R.…”

Section: Brain Sample Preparationmentioning

confidence: 99%

Influence of Cerebral Glucose Metabolism by Chronic Pain–Mediated Cognitive Impairment in Adolescent Rats

et al. 2022

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Chronic pain during adolescence can lead to mental health disorders in adulthood, but the underlying mechanism is still unclear. Furthermore, the homeostasis of cerebral glucose metabolism and neurotransmitter metabolic kinetics are closely associated with cognitive development and pain progression. The present study investigated changes in cognitive function and glucose metabolism in adult rats, which had experienced chronic pain during their adolescence. Here, spared nerve injury (SNI) surgery was conducted in 4-week-old male rats. Mechanical nociceptive reflex thresholds were analyzed, and SNI chronic pain (SNI-CP) animals were screened. Based on animal behavioral tests (open field, three-chambered social, novel object recognition, and the Y maze), the SNI-CP animals showed learning and memory impairment and anxiety-like behaviors, compared to SNI no chronic pain (SNI-NCP) animals. The cerebral glucose metabolism in the prefrontal cortex and hippocampus of adult SNI-CP animals was decreased with positron emission tomography/computed tomography. GABA2 and Glu4 levels in the metabolic kinetics study were significantly decreased in the hippocampus, frontal cortex and temporal cortex, and the expression of GLUT3 and GLUT4 was also significantly downregulated in the prefrontal cortex and hippocampus of adult rats in the SNI-CP group. These findings suggest that the rats which suffered chronic pain during adolescence have lower cerebral glucose metabolism in the cortex and hippocampus, which could be related to cognitive function during the development of the central nervous system.

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“…After an adequate level of anesthesia was provided, all rats were catheterized in the lateral tail vein via a PE50 cannula (Instech, PA, USA) with a needle tip and fastened with paper adhesive tape. The other side of the canula was attached to a syringe filled with saline [11]. After that the animal was moved to a new cage for recovery.…”

Section: Infusion Of [1-13 C] Glucosementioning

confidence: 99%

Identification of metabolic kinetic patterns in different brain regions using metabolomics methods coupled with various discriminant approaches

Zheng

Zhao

et al. 2021

Journal of Pharmaceutical and Biomedical Analysis

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Regional cerebral metabolic levels and turnover in awake rats after acute or chronic spinal cord injury

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 12 publications

References 55 publications

Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique

Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique

Influence of Cerebral Glucose Metabolism by Chronic Pain–Mediated Cognitive Impairment in Adolescent Rats

Identification of metabolic kinetic patterns in different brain regions using metabolomics methods coupled with various discriminant approaches

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