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Non-technical summaryEMU is accompanied by increased market integration and factor mobility -especially of the production factor capital. Up to date, there is no clear indication concerning the specialisation tendencies to be expected due to these impacts. This paper thus investigates the driving forces and differences of sectoral investment and employment specialisation of EU regions.The econometric analyses aim at identifying the regional determinants of high relative sectoral specialisation, i.e. the regional investment or employment specialisation in different sectors. The importance of a number of determinants from different theoretical approaches is tested controlling for heteroscedasticity and potential endogeneity.Sectoral productivity differentials between regions generally (mostly) contribute to the explanation of relative investment (employment) shares in those nine manufacturing sectors analysed. Striking is the consistent significance of low (high) regional labour cost levels in explaining high employment shares in labour-intensive (human capital-intensive) sectors. This is less evident for the explanation of investment shares. But to sum up, one can see that in many sectors, productivity differentials and average regional labour cost differentials contribute to the explanation of specialisation patterns in accordance with traditional trade theory. Productivity differentials do so, especially with respect to the explanation of investment patterns, regional labour cost differentials with respect to the explanation of employment patterns.For manufacturing sectors, the location close to large markets seems to matter according to the predictions of the New Economic Geography. We might also be confronted with further agglomeration potential in the manufacturing sectors, since the regional level of sectorspecific economies of scale is consistently significant and positive for the manufacturing sectors. However, market integration, which is supposed to enforce the agglomerative forces of economies of scale does not play a particular role in specific sectors.Additional country-specific effects differ with respect to each sector concerning investment specialisation, but clear country-specific effects are evident for employment patterns. Italy shows significantly lower employment shares in a number of manufacturing sectors and higher ones in the labour-intensive sectors agriculture and trade & lodging.In earlier studies...