Abstract:Serotonin synthesis rates were evaluated using a-[ 14 C]methyl-L-tryptophan (a-MTrp) autoradiographic methods in olfactory bulbectomized (OBX) rats. They were significantly (p < 0.05) increased in the frontal (50%) and parietal (40%) cortices, superior olive (over 30%), and the substantia nigra (30%) in the OBX rats as compared to the sham operated animals. There were also increases in 5-hydroxytryptamine (5-HT) synthesis in some limbic areas: the cingulate (32%), the medial forebrain bundle (58%), the hippoca… Show more
“…39 Studies have also shown that 5-HT 1A receptors are supersensitive in the FSL rats when compared to the SPD controls. 16,17 Further, 5-HT synthesis has been identified as a parameter that is affected by OBX 40,41 and is normalized by antidepressant treatments. 27,41 As noted, we concentrate on the OBX and FSL rat models of depression in this review, because our group has acquired a substantial volume of data on the serotonergic system in these two models, and that data is not available on any other animal model.…”
Section: Skelin Et Al Neurochemical and Behavioural Changes In Rmentioning
confidence: 99%
“…40,49 In brief, Sprague-Dawley (SPD) rats (weighting 200-250 g) are anaesthetized with inhalatory anaesthetics, isoflurane or halothane, and the small hole is opened on the frontal bone, 5.2 mm anterior from the bregma and approximately 2 mm on both sides from the midline. The olfactory bulbs are visualized with a surgical microscope, cut and aspirated with a dull needle attached to a vacuum water pump, taking care not to damage the frontal lobes.…”
Section: Surgical Procedures and Its Subsequent Neuroanatomical Implicmentioning
confidence: 99%
“…50 Olfactory bulbectomy-induced changes, in many brain regions, are the result of direct or indirect connectivity of those regions with olfactory bulbs. 24,40 Subsequent extensive changes were referred to retrograde, anterograde and transneuronal degeneration, including the damages that are a result of vascular blood supply deprivation. 24 In addition to the bulbectomy-associated axotomy of 5-HT fibres, the reorganization of 5-HT projections to the forebrain is continued by collateral sprouting and synaptogenesis.…”
Section: Surgical Procedures and Its Subsequent Neuroanatomical Implicmentioning
Abstract. Depression is one of the most prevalent psychiatric disorders with significant lifetime prevalence. The study of human depression is hindered by the relatively significant heterogeneity of this disorder. As a result of these heterogeneities, studies using a sound and representative animal model of depression are extremely valuable and, in general, provide important information regarding the neurochemical/biological changes occurring during depression and the subsequent treatment of its symptoms. In this review we concentrate on two solid rat models of depression: olfactory bulbectomized (OBX) rats and Flinders Sensitive Line (FSL) of rats. These two models were selected because they possess all three characteristics of a good animal model; face, predictive and constructive validities. We also emphasize the brain serotonergic system and we discuss the open field and forced swim test to assess behaviour. (doi: 10.5562/cca1817) Keywords: depression, olfactory bulbectomized rat model of depression, Flinders sensitive line rat, Flinders Resistant line rat, animal model of depression
“…39 Studies have also shown that 5-HT 1A receptors are supersensitive in the FSL rats when compared to the SPD controls. 16,17 Further, 5-HT synthesis has been identified as a parameter that is affected by OBX 40,41 and is normalized by antidepressant treatments. 27,41 As noted, we concentrate on the OBX and FSL rat models of depression in this review, because our group has acquired a substantial volume of data on the serotonergic system in these two models, and that data is not available on any other animal model.…”
Section: Skelin Et Al Neurochemical and Behavioural Changes In Rmentioning
confidence: 99%
“…40,49 In brief, Sprague-Dawley (SPD) rats (weighting 200-250 g) are anaesthetized with inhalatory anaesthetics, isoflurane or halothane, and the small hole is opened on the frontal bone, 5.2 mm anterior from the bregma and approximately 2 mm on both sides from the midline. The olfactory bulbs are visualized with a surgical microscope, cut and aspirated with a dull needle attached to a vacuum water pump, taking care not to damage the frontal lobes.…”
Section: Surgical Procedures and Its Subsequent Neuroanatomical Implicmentioning
confidence: 99%
“…50 Olfactory bulbectomy-induced changes, in many brain regions, are the result of direct or indirect connectivity of those regions with olfactory bulbs. 24,40 Subsequent extensive changes were referred to retrograde, anterograde and transneuronal degeneration, including the damages that are a result of vascular blood supply deprivation. 24 In addition to the bulbectomy-associated axotomy of 5-HT fibres, the reorganization of 5-HT projections to the forebrain is continued by collateral sprouting and synaptogenesis.…”
Section: Surgical Procedures and Its Subsequent Neuroanatomical Implicmentioning
Abstract. Depression is one of the most prevalent psychiatric disorders with significant lifetime prevalence. The study of human depression is hindered by the relatively significant heterogeneity of this disorder. As a result of these heterogeneities, studies using a sound and representative animal model of depression are extremely valuable and, in general, provide important information regarding the neurochemical/biological changes occurring during depression and the subsequent treatment of its symptoms. In this review we concentrate on two solid rat models of depression: olfactory bulbectomized (OBX) rats and Flinders Sensitive Line (FSL) of rats. These two models were selected because they possess all three characteristics of a good animal model; face, predictive and constructive validities. We also emphasize the brain serotonergic system and we discuss the open field and forced swim test to assess behaviour. (doi: 10.5562/cca1817) Keywords: depression, olfactory bulbectomized rat model of depression, Flinders sensitive line rat, Flinders Resistant line rat, animal model of depression
“…As several reports have linked olfactory bulbectomy to changes in the amygdala and hippocampus (Richardson and Tiong, 1999, elevated density of b-adrenoceptors in the amygdala and hippocampus; Wrynn et al, 2000, decreased MRI signal in the amygdala, indicating an altered tissue volume, pronounced enlargement of the lateral ventricle with impaired hippocampal function as a consequence; Rutkoski et al, 2002, significant increase in neuropeptide Y expression in the amygdala; Watanabe et al, 2003, increased serotonin synthesis in the hippocampus), we selected the two brain areas for this study. Moreover, the hippocampus and amygdala besides the anterior cingulate and the prefrontal cortex are included into the key neural circuit of depression (Henn and Vollmayr, 2004b;Sapolsky, 2004).…”
Growing evidence indicates that alterations of neuroplasticity may contribute to the pathophysiology of depression. In contrast, various antidepressants increase adult hippocampal neurogenesis and block the effects of stress. These findings result in the 'neurogenesis hypothesis of depression'. The present study seeks to determine out whether cell proliferation is altered in the hippocampus, subventricular zone (SVZ), and basolateral amygdala of adult rats exposed to bilateral olfactory bulbectomy, another established model of depression and, if so, how imipramine effects bulbectomy-induced changes of cell genesis. Bulbectomy results in a significant reduction of cell proliferation in the hippocampus and SVZ, an effect that is normalized by subchronic doses of imipramine. Moreover, an increase in cell genesis in the basolateral amygdala, which is not affected by imipramine, is demonstrated. TUNEL staining indicates an enhanced apoptosis after bulbectomy in the SVZ that cannot be reduced by imipramine. Cell death rates in the hippocampus and amygdala are not affected by bulbectomy. The opposing effects of bulbectomy and imipramine treatment in the hippocampus and amygdala demonstrate that these structures of the limbic system, both integrated in emotional processing, react quite differently with regard to neuroplasticity. Further to this, we discuss a possible link between the pathogenesis of depression and changed neuronal plasticity in the SVZ.
“…Atualmente, a buspirona é utilizada no tratamento da ansiedade. Entretanto, estudos demonstram que pode haver alterações nas funções do sistema serotoninérgico durante a patogê-nese da epilepsia 3,4 , despertando, assim, o interesse na investigação da buspirona em modelos de epilepsia.…”
ResumoContexto: Crises epilépticas induzidas por pilocarpina podem produzir alterações histopatológicas em muitas regiões cerebrais como consequência da produção excessiva de radicais livres. Objetivo: O objetivo do presente estudo foi avaliar o efeito antioxidante da buspirona no modelo de epilepsia induzida por pilocarpina. Material e métodos: Quarenta e oito animais foram divididos em quatro grupos. O primeiro grupo foi tratado com solução salina 0,9% (Controle). O segundo grupo foi tratado com pilocarpina 400 mg/kg (P400). Por sua vez, o terceiro grupo foi tratado com buspirona 5 mg/kg (BUSP) durante 14 dias consecutivos. Já os animais do quarto grupo foram tratados com buspirona durante 14 dias consecutivos, e, 30 minutos após a última administração dela, os camundongos receberam P400 (BUSP + P400). Resultados: Durante o período do tratamento não se observaram sinais de toxicidade e nenhuma morte entre os animais tratados com buspirona. Em nosso estudo o grupo tratado com P400 demonstrou um aumento signi cativo da produção de nitrito e nos níveis de peroxidação lipídica após as crises epilépticas. Por outro lado, no hipocampo dos animais que receberam o pré-tratamento com buspirona e após 30 minutos receberam P400, foi observada redução signi cativa nos níveis de peroxidação lipídica (65%) e nitrito (85%), bem como aumento na atividade da enzima superóxido dismutase. Conclusão: O pré-tratamento com BUSP aumentou a latência para primeira crise epiléptica e diminuiu a taxa de mortalidade e o número de animais que apresentaram crise epiléptica e que progridem para o estado de mal epiléptico. Além disso, apresentou efeitos anticonvulsivantes associados com a redução do estresse oxidativo hipocampal no modelo de epilepsia induzida por pilocarpina.
AbstractBackground: Pilocarpine-induced seizures can cause pathological changes in many brain regions as a result of excessive production of free radicals. Objective: e objective of this study was to evaluate the antioxidant e ect of buspirone in the epilepsy model induced by pilocarpine. Material and methods: Forty-eight animals were divided into four groups. e rst group was treated with saline 0.9% (control); the second group received pilocarpine 400 mg/kg (P400); the third group was treated with buspirone 5 mg/kg (BUSP) for 14 consecutive days and animals in the fourth group were treated with buspirone for 14 consecutive days, and 30 minutes a er the last buspirone administration were administered with P400 (BUSP + P400). Results: No toxicity signs or death were observed in buspirone-treated animals. P400 group showed a signi cant increase in nitrite production and lipid peroxidation a er seizures. Moreover, reduction in both the lipid peroxidation level (65%) and nitrite content (85%) as well as an increase in superoxide dismutase activity was detected following P400 injection in the hippocampus of buspirone-pretreated mice. Discussion: Pretreatment with BUSP increased latency to rst seizure, decreased the mortality rate and number of animals that presented seizur...
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