Region‐specific regulation of the serotonin 2A receptor expression in development and ageing in post mortem human brain

Abstract

Our results show plasticity and region specificity of HTR2A expression regulation in human brain with age, which may be important for the interaction with other neurotransmitter systems and for the occurrence of developmental periods with increased vulnerability to neuropsychiatric or neurodegenerative disorders.

5‐hydroxytryptamine1A receptor density in the hippocampus of patients with temporal lobe epilepsy is associated with disease duration

“…In our study, we measured 5‐HT1AR density using western blotting, a technique often used for protein detection . Western blotting is used to evaluate the protein density of different types of receptors in animal and human cortical tissue , including serotonergic receptors . Few studies have evaluated serotonergic receptors in human cortical tissue.…”

Novel miR-b2122 regulates several ALS-related RNA-binding proteins

“…Interestingly, RGNEF protein levels went in the opposite direction of the mRNA levels within our study. While rare to see inverse correlations between mRNA and protein levels of a single gene, it is not unprecedented [ 44 , 45 ]. This could imply when miR-b2122 binds to the RGNEF 3’UTR, its role is to maintain low levels of mRNA while keeping the transcript in a translationally stable state, and thus, loss of its binding stabilizes the mRNA molecule, but leaves the transcript in a translationally silent state.…”

The Cerebellar Dopaminergic System

“…Moreover, DAT immunoreactive nerve fibers variously oriented in the subcortical white substance, has been detected (Figure 1; Flace et al, 2019aFlace et al, ,b, 2020. In the dentate nucleus, the DAT immunoreactivity in neuronal cell bodies and processes of different neuron types has been detected (Table 1; Figure 3); the small neuron type is involved in intrinsic circuits, the medium neuron type (Table 1; Figure 3) mainly involved in intrinsic and also in extrinsic circuits, and four different large neuron types, which include the central neuron, the border neuron, the intermediate asymmetrical neuron, and the intermediate fusiform neuron, involved in projective circuits of the dentate nucleus (data not showed; Chan-Palay, 1977;Maric, 2010;Ristanović et al, 2010;Flace et al, 2017Flace et al, , 2019bFlace et al, , 2020Flace, 2018). Dopamine transporter immunoreactivity in neuronal cell bodies and processes of the perivascular neuron type has also been observed, a neuron type may be involved in regulatory mechanisms of blood-brain barrier (BBB) permeability and in volume transmission mechanisms (data not showed; Flace et al, 2004;Ambrosi et al, 2007;Flace, 2017Flace, , 2018Flace, , 2019bFlace, , 2020.…”

“…The cerebellar serotonergic fibers originate mostly by the serotonergic cell groups of the reticular formation (B 1 -B 3 , B 6 , B 7 , and B 9 ; Dahlström and Fuxe, 1964;Bishop and Ho, 1985;Törk, 1990, Kerr andBishop, 1991;Bishop, 1994, 1997). In the cerebellar cortex and the deep cerebellar nuclei, different serotonergic subtype receptors such as 5-HT 1B , 5-HT 2A , 5-HT 2B , 5-HT 3 , and 5-HT 5A have been demonstrated (Duxon et al, 1997;Pasqualetti et al, 1998;Sari et al, 1999;Geurts et al, 2002;Oostland et al, 2013;Marinova et al, 2015). During the development, a role of 5-HT in dendritic growth and synaptic plasticity mechanisms has been demonstrated (Bishop et al, 1988;Oostland and van Hooft, 2013;Oostland et al, 2013).…”