Regenerative treatment using a radioelectric asymmetric conveyor as a novel tool in antiaging medicine: an in vitro beta-galactosidase study

Abstract

BackgroundBeta-galactosidase is the most widely used biomarker for highlighting the processes of cellular aging, including neurodegeneration. On this basis, we decided to test in vitro whether a set of rescuing/reparative events previously observed by us in subjects treated with radioelectric asymmetric conveyor (REAC) technology may also involve antagonism of a marker of aging-related degenerative processes, as assessed by a reduction in beta-galactosidase at the cellular level.MethodsHuman adipose-derived stem cells were cultured at different passages, ranging from 5 to 20, with or without REAC exposure for 12 hours. The cells were then processed for biochemical beta-galactosidase staining and morphological microscopy analysis.ResultsWe observed a significant reduction in expression of senescence associated-beta-galactosidase, and a persistence of fibroblast-like morphology typical of human adipose-derived stem cells, even at late passages.ConclusionOur results indicate the ability of REAC technology to counteract in vitro senescence of human adipose-derived stem cells, and prompt the hypothesis that such technology may be exploited to antagonize in vivo senescence of tissue-resident or transplanted stem cells playing an important role in clinical treatment of age-related processes.

Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson’s disease

“…REAC tissue optimization (TO) regenerative treatments (RGN) includes a set of treatment protocols 1 2 3 4 5 20 which are carried out by the ACP immersed in the medium of the cell culture or covering the treated area with ACP. The REAC TO-RGN has been administered 168 hours without interruption.…”

“…Previous studies, conducted using the radio electric asymmetric conveyor (REAC) technology, have shown that this technology is able to induce neurogenic cell differentiation both in cultures of murine embryonic cells 1 and in differentiated human cells, such as fibroblasts 2 and adipocytes 3 . In addition, the REAC technology has been shown to effectively counteract cell aging 4 5 6 , a process often related to neurodegenerative diseases as Parkinson’s disease (PD). To better dissect and understand the potential of the REAC treatments in PD, we chose a PC 12 cellular model.…”

Stem cell senescence. Effects of REAC technology on telomerase-independent and telomerase-dependent pathways

“…Furthermore, REAC TO-RGN exposure proved effective in antagonizing the aging process in vitro 333435. Here, we show that REAC TO-RGN exposure also affected telomerase-dependent senescence mechanisms, by transcriptionally enhancing the expression of TERT, and counteracting telomere shortening.…”

Unravelling Cellular Mechanisms of Stem Cell Senescence: An Aid from Natural Bioactive Molecules

“…For these reasons, researchers in recent years have been trying to dissect the epigenetic modifications involved in cellular aging, in an attempt to reverse stem cell senescence and achieve rejuvenation [32,33]. Physical stimuli such as electromagnetic fields have been largely applied to slow down cell senescence, by modulating cell polarity and the expression of the aging related markers, such as TERT, p16INK4, ARF, p53, and p21, together with the activity of beta-galactosidase [21,[34][35][36]. Moreover, other authors demonstrated that Rapamycin can inhibit mTOR and its signalling pathway, restoring both the self-renewal and the potency of stem cells [37].…”