Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson’s disease

Maioli, Margherita; Rinaldi, Salvatore; Migheli, Rossana; Pigliaru, Gianfranco; Rocchitta, Gaia; Santaniello, Sara; Basoli, Valentina; Castagna, Alessandro; Fontani, Vania; Ventura, Carlo; Serra, Pier Andrea

doi:10.1038/srep10439

Cited by 40 publications

(39 citation statements)

References 24 publications

(38 reference statements)

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“…It was also found that 1800 MHZ exposure of mouse embryonic neural stem cells, with a specific absorption rate (SAR) of 4 W/kg, could decrease neurite outgrowth [23]. Lately, another team evidenced that radio electric asymmetric conveyor (REAC) technology, involving a 2.4 GHz exposure (SAR = 0.128 W/kg), induced a shift of the exposed PC12 cells toward the neurogenic phenotype, characterized by an increased protein expression of the neuronal marker βIII-tubulin [24].…”

Section: Introductionmentioning

confidence: 99%

Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening

Haas

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Sauleau

et al. 2016

Neuroscience Letters

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Highlights: 60 GHz MMW do not impact neuronal tubulin expression during PC12 differentiation. Exposure at 10 mW/cm² induces a slight increase in neurite outgrowth due to the thermal effect. The thermal effect impacts all parameters of the neurite outgrowth. AbstractTechnologies for wireless telecommunication systems using millimeter waves (MMW) will be widely deployed in the near future. Forthcoming applications in this band, especially around 60 GHz, are mainly developed for high data-rate local and body-centric telecommunications. At those frequencies, electromagnetic radiations have a very shallow penetration into biological tissues, making skin keratinocytes, and free nerve endings of the upper dermis the main targets of MMW. Only a few studies assessed the impact of MMW on neuronal cells, and none of them investigated a possible effect on neuronal differentiation.We used a neuron-like cell line (PC12), which undergoes neuronal differentiation when treated with the neuronal growth factor (NGF). PC12 cells were exposed at 60.4 GHz for 24 h, at an incident power density averaged over the cell monolayer of 10 mW/cm². Using a large scale cell-by-cell analysis based on high-content screening microscopy approach, we assessed potential effects of MMW on PC12 neurite outgrowth and cytoskeleton protein expression. No differences were found in protein expression of the neuronal marker β3-Tubulin nor in internal expression control β-Tubulin. On the other hand, our data showed a slight increase, although insignificant, in neurite outgrowth, induced by MMW exposure.However, experimental controls demonstrated that this increase was related to heating.Abbreviations: MMW (millimeter waves), HC (heat control).

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Section: Introductionmentioning

confidence: 99%

Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening

Haas

Page

Sauleau

et al. 2016

Neuroscience Letters

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“…[ 16 , 41 ]). In addition, experimental field studies indicate a profound role for complex interactions across scales governing community dynamics [ 19 , 42 , 43 ]. The MEM-framework offers a quantitative approach towards understanding scale-dependent community interactions associated with abiotic variables (also see, e.g.…”

Section: Discussionmentioning

confidence: 99%

Cross-Scale Variation in Biodiversity-Environment Links Illustrated by Coastal Sandflat Communities

et al. 2015

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Spatial variation in the composition of communities is the product of many biotic and environmental interactions. A neglected factor in the analysis of community distribution patterns is the multi-scale nature of the data, which has implications for understanding ecological processes and the development of conservation and environmental management practice. Drawing on recently established multivariate spatial analyses, we investigate whether including relationships between spatial structure and abiotic variables enable us to better discern patterns of species and communities across scales. Data comprised 1200 macrozoobenthic samples collected over an array of distances (30 cm to 1 km) in three New Zealand harbours, as well as commonly used abiotic variables, such as sediment characteristics and chlorophyll a concentrations, measured at the same scales. Moran’s eigenvector mapping was used to extract spatial scales at which communities were structured. Benthic communities, representing primarily bivalves, polychaetes and crustaceans, were spatially structured at four spatial scales, i.e. >100 m, 50–100 m, 50–15 m, and < 15 m. A broad selection of abiotic variables contributed to the large-scale variation, whereas a more limited set explained part of the fine-scale community structure. Across all scales, less than 30% of the variation in spatial structure was captured by our analysis. The large number of species (48) making up the 10 highest species scores based on redundancy analyses illustrate the variability of species-scale associations. Our results emphasise that abiotic variables and biodiversity are related at all scales investigated and stress the importance of assessing the relationship between environmental variables and the abundance and distribution of biological assemblages across a range of different scales.

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“…Compounding this picture, electromagnetic fields have been shown to afford neurological and morphofunctional differentiation in PC 12 cells [40], a rat adrenal pheochromocytoma cell line displaying metabolic features of Parkinson's disease. The action of electromagnetic fields was mediated by the transcriptional activation of neurogenic genes, as neurogenin-1, β3-tubulin and nerve growth factor (NGF), and was associated with a consistent increase in the number of cells expressing both β3-tubulin and tyrosine hydroxylase [40].…”

Section: S4mentioning

confidence: 99%

“…The action of electromagnetic fields was mediated by the transcriptional activation of neurogenic genes, as neurogenin-1, β3-tubulin and nerve growth factor (NGF), and was associated with a consistent increase in the number of cells expressing both β3-tubulin and tyrosine hydroxylase [40]. More recently, we have shown that the antiaging effect of REAC conveyed electromagnetic fields could be significantly opposed by stem cell exposure to an inhibitor of type 2 hyaluronan synthase (HAS2) [41].…”

Section: S4mentioning

confidence: 99%

Seeing Cell Biology with the Eyes of Physics

Ventura¹

2017

NanoWorld J

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Rhythmic oscillatory patterns permeate the entire universe and sustain cellular dynamics at biological level. The intracellular environment is now regarded as a complex nanotopography embedding "intracrine" signals that encompass the intracellular action of regulatory molecules coupled with the newly discovered functions of the microtubuar network. Microtubuli, are far away from simply being a part of the cytoskeleton, since they produce both nanomechanical motions and display electromagnetic resonance modes that may turn local events into non-local, long-ranging paths. The possibility that microtubuli form a bioelectronic circuit prompts rethinking of biomolecular recognition as a result of synchronization of the oscillatory patterns of proteins sustained and orchestrated by resonance modes brought about by the microtubular network itself. Here, we discuss these issues within the biomedical perspective of using physical energies to govern (stem) cell fate. We focus on the ability of specially conveyed electromagnetic fields to afford optimization of stem cell polarity and pluripotency, reversing stem cell aging and promoting a multilineage repertoire in human adult stem cells, as well as in human non-stem somatic cells. We discuss the use atomic force microscopy and hyperspectral imaging for deciphering the nanomotions generated by (stem) cells during their growth and differentiation. We highlight the potential for unraveling vibrational signatures that can be exploited to direct the differentiation and self-healing potential of tissue-resident stem cells in vivo. In conclusion, seeing stem cell biology with the eyes of Physics may help developing a Regenerative/Precision medicine afforded through the stimulation of the natural ability of tissues for self-healing, without the needs of stem cell transplantation.

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Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson’s disease

Cited by 40 publications

References 24 publications

Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening

Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening

Cross-Scale Variation in Biodiversity-Environment Links Illustrated by Coastal Sandflat Communities

Seeing Cell Biology with the Eyes of Physics

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