Regenerative Properties of Recombinant Human Erythropoietin in the Wound Healing

Toleubayev, Medet; Dmitriyeva, Mariya; Kozhakhmetov, Saken; Igissinov, Nurbek; Turebayev, Dulat; Omarbekov, Ardak; Adaibayev, Kairat; Shakenov, Abylay; Izimbergenov, Mirsaid

doi:10.3889/oamjms.2021.5813

Cited by 3 publications

(4 citation statements)

References 30 publications

(44 reference statements)

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“…Tobalem et al [ 62 ] also informed a dose-dependent positive effect of systemic EPO early treatment reducing inflammation and accelerating healing of burn wounds. Toleubayev et al [ 63 ] also stated a positive effect of EPO in several wound healing models, for instance traumatized wounds, infected wounds, chronic ischemic wounds, and diabetic ones where EPO significantly reduced tissue inflammation and accelerated the blood vessels regeneration.…”

Section: Discussionmentioning

confidence: 99%

Healing of induced tongue defects using erythropoietin hydrogel (an experimental study on rats)

Ata,

El-Qashty,

Farid

et al. 2024

BMC Oral Health

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Background Tongue is complex muscular organ that may be affected by recurrent or chronic ulcerations and malignances that require effective treatment to enhance healing and tissue regeneration. So, this study aimed to evaluate the efficiency of erythropoietin (EPO) hydrogel as an anti-inflammatory and an inducer of neovascularization during healing of induced rats’ tongue defects. Methods Thirty six rats were divided into three groups; Group I (negative control): tongues were left without ulceration and received no treatment, Group II (positive control): tongue defects were prepared on the tongues’ dorsal surfaces, measuring (5 mm × 2 mm) using a tissue punch rotary drill for standardization, and left untreated, Group III (EPO group): tongue defects were prepared as in group II, then injected circumferentially around wound margins with a single high dose of EPO hydrogel of 5000 U/kg on the day of defect preparation. Animals were euthanized on seventh and fourteenth days after treatment, tongue specimens were collected, and paraffin blocks were prepared and processed for histological assessment by hematoxylin and eosin stain and immunohistochemical evaluation of anti-iNOS and anti-VEGF followed by histomorphometrical analysis and the relevant statistical tests. Results At both time points, the EPO treated group showed significantly enhanced tissue regeneration marked by the histologically better regenerated tissue with well developed, thick walled and well-organized blood vessels and significant reduction in defect depth compared to positive control group. EPO group also showed significant decrease in iNOS and significant increase in VEGF antibodies indicating its anti-inflammatory and neovascularization effects respectively. Conclusion EPO treatment can significantly accelerate regeneration and filling of tongue defects by reducing tissue inflammation and enhancing neovascularization. Therefore, EPO could be a potential therapeutic strategy for accelerating healing of tongue ulcers. However, further investigations are required to optimize the dose and unravel any potential side effects before its clinical application.

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Section: Discussionmentioning

confidence: 99%

Healing of induced tongue defects using erythropoietin hydrogel (an experimental study on rats)

Ata,

El-Qashty,

Farid

et al. 2024

BMC Oral Health

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“…The activation, migration, and proliferation of endothelial cells, as well as the maturation of cell junctions and the surrounding basement membrane, all contribute to the development of new functional capillary structures. VEGF influences each of these phases [14]. Hence, indirectly, giving erythropoietin can trigger the emergence of VEGF.…”

Section: Discussionmentioning

confidence: 99%

“…Erythropoietin is a glycoprotein hormone that can stimulate angiogenesis [11]. Various studies have been conducted to prove that the administration of erythropoietin accelerates the speed of wound healing [12], [13], [14]. In 2010, Hamed et al found that topical erythropoietin treatment quadrupled the speed of wound repair in diabetic rats with full-thickness wounds and reduced cell apoptosis [15].…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor, Epidermal Growth Factor, and Epithelialization Analysis on Full-Thickness Wound Applied with Topical Erythropoietin

Sari

Saputro²,

Hutagalung³

2022

Open Access Maced J Med Sci

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BACKGROUND: Skin wounds are a major challenge, such as full-thickness wounds that need a long time to heal. However, the addition of erythropoietin can accelerate the wound healing process. AIM: This study was aimed to determine the effect of topical erythropoietin administration on vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and epithelialization in the wound healing process. METHODS: This was randomized controlled trial. Full-thickness wounds were created on the back of each white rat. No treatment was administered in the control group (n=16), and topical erythropoietin was applied in the treatment group (n=16). The rats were euthanized on day 3 and day 6 post-surgery, respectively. The expression levels of VEGF, EGF, and microscopic epithelialization rate were examined. RESULTS: The level of EGF expression in the treatment group increased significantly on day 3 by 2.84 times compared to the control group and on day 6 increased to 4.89 times compared to the control group (p < 0.001). The level of VEGF expression in the treatment group on day 3 increased 2.18 times compared to the control group and on day 6 increased to 2.65 times compared to the control group (p < 0.001). Meanwhile, epithelialization in the treatment group on day 3 increased 1.3 times compared to the control group and on day 6 increased up to 7.62 times compared to the control group (p < 0.001). CONLUSION: Topical administration of erythropoietin could increase the expression of VEGF, EGF, and epithelialization in both early inflammatory and proliferative phases.

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“…Wound healing is a natural physiological response to tissue damage. In general, wound healing is performed with four specific stages of hemostasis, inflammation, proliferation, and maturation (Figure 1) [8][9][10]. ECM plays a crucial role in every step of the wound healing process.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Agent-Loaded Electrospun Nanofiber Membranes for Accelerating Healing Process: A Review

et al. 2021

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Despite the advances that have been achieved in developing wound dressings to date, wound healing still remains a challenge in the healthcare system. None of the wound dressings currently used clinically can mimic all the properties of normal and healthy skin. Electrospinning has gained remarkable attention in wound healing applications because of its excellent ability to form nanostructures similar to natural extracellular matrix (ECM). Electrospun dressing accelerates the wound healing process by transferring drugs or active agents to the wound site sooner. This review provides a concise overview of the recent developments in bioactive electrospun dressings, which are effective in treating acute and chronic wounds and can successfully heal the wound. We also discuss bioactive agents used to incorporate electrospun wound dressings to improve their therapeutic potential in wound healing. In addition, here we present commercial dressings loaded with bioactive agents with a comparison between their features and capabilities. Furthermore, we discuss challenges and promises and offer suggestions for future research on bioactive agent-loaded nanofiber membranes to guide future researchers in designing more effective dressing for wound healing and skin regeneration.

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Regenerative Properties of Recombinant Human Erythropoietin in the Wound Healing

Cited by 3 publications

References 30 publications

Healing of induced tongue defects using erythropoietin hydrogel (an experimental study on rats)

Healing of induced tongue defects using erythropoietin hydrogel (an experimental study on rats)

Vascular Endothelial Growth Factor, Epidermal Growth Factor, and Epithelialization Analysis on Full-Thickness Wound Applied with Topical Erythropoietin

Bioactive Agent-Loaded Electrospun Nanofiber Membranes for Accelerating Healing Process: A Review

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