Regenerative implants for cardiovascular tissue engineering

Lee, Avione Y.; Mahler, Nathan; Best, Cameron; Lee, Yong‐Ung; Breuer, Christopher K.

doi:10.1016/j.trsl.2014.01.014

Cited by 44 publications

(32 citation statements)

References 170 publications

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“…Because of biocompatible degradation properties, PCL is approved for use in human beings by the US Food and Drug Administration 22. Gelatin is a mixture of peptides and proteins produced by partial hydrolysis of collagen extracted from animals.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium

Wang

et al. 2017

J Cellular Molecular Medi

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Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF‐1α, Tβ4, VEGF and SDF‐1 expression and decreased CXCL14 expression in hypoxic and serum‐deprived conditions. In murine MI models, the survival and distribution of the engrafted MSCs and the activation of the epicardium were examined, respectively. At 4 weeks after transplantation of the cell patch, the cardiac functions were significantly improved. The engrafted MSCs migrated across the epicardium and into the myocardium. Tendency of HIF‐1α, Tβ4, VEGF, SDF‐1 and CXCL14 expression in the infarcted myocardium was similar with expression in vitro. The epicardium was activated and epicardial‐derived cells (EPDCs) migrated into deep tissue. The EPDCs differentiated into endothelial cells and smooth muscle cells, and some of EPDCs showed to have differentiated into cardiomyocytes. Density of blood and lymphatic capillaries increased significantly. More c‐kit+ cells were recruited into the infarcted myocardium after transplantation of the cell patch. The results suggest that epicardial transplantation of the cell patch promotes repair of the infarcted myocardium and improves cardiac functions by enhancing the survival of the transplanted cells, accelerating locality paracrine, and then activating the epicardium and recruiting endogenous c‐kit+ cells. Epicardial transplantation of the cell patch may be applied as a novel effective MI therapy.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium

Wang

et al. 2017

J Cellular Molecular Medi

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“…Transplantation of vascular cells has tremendous potential for vascular regeneration either by restoring blood flow to ischemic tissues or implanting a tissue engineered blood vessel [70, 71]. So far no clinical trials have been performed using these cells due to a concern for potential tumorigenicity abilities of these cells [72–74].…”

Section: Regenerative Therapymentioning

confidence: 99%

Induced pluripotent stem cell-derived vascular smooth muscle cells: methods and application

Dash

Jiang

Suh

et al. 2015

Biochemical Journal

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Vascular smooth muscle cells (VSMCs) play a major role in the pathophysiology of cardiovascular diseases. The advent of induced pluripotent stem cell (iPSC) technology and their capability to differentiation into virtually every cell type in the human body make this field a ray of hope for vascular regenerative therapy and for understanding disease mechanism. In this review, we first discuss the recent iPSC technology and vascular smooth muscle development from embryo and then examine different methodology to derive VSMCs from iPSCs and their applications in regenerative therapy and disease modeling.

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“…Scaffolds can be generated from either synthetic matrices, such as polylactic acid and polyglycolic acid, which are often used for simpler constructs, or from biologically derived matrices, such as fibrin, collagen and tissue ECM. For synthetic matrices, properties such as composition, porosity, biodegradation rate and architecture can be precisely controlled, but these materials often fail to recapitulate the properties of native tissue and can be poor substrates for cell attachment and growth (2). In contrast, biologically derived ECM matrices, if left in a native state, can provide natural cues for cell migration, proliferation and differentiation, but scaffolds made from single ECM components often lack the sophistication to develop into a functional tissue.…”

Section: Scaffoldsmentioning

confidence: 99%