Building New Hearts: A Review of Trends in Cardiac Tissue Engineering

Taylor, Doris A.; Sampaio, Luiz C.; Gobin, Andrea S.

doi:10.1111/ajt.12939

Cited by 51 publications

(35 citation statements)

References 51 publications

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“…6,25,26 As stated by Taylor et al 27 as one of the pioneers in developing whole organ decellularization, the improvement of the decellularization process to produce ECM from deceased organs is one of the most notable achievements in the field of tissue engineering within the past 7 years. Acellular ECM can be obtained by decellularization of native tissue either by perfusing detergent solutions through the vasculature or diffusion of this solution through pieces of tissue.…”

Section: Discussionmentioning

confidence: 99%

Using Hemolysis as a Novel Method for Assessment of Cytotoxicity and Blood Compatibility of Decellularized Heart Tissues

et al. 2016

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Developing patient-specific transplantable organs is a promising response to the increasing need of more effective therapies for patients with organ failure. Advances in tissue engineering strategies have demonstrated favorable results, including the use of decellularized hearts as scaffolds for cardiac engineering; however, there is a need to establish methods to characterize the cytotoxicity and blood compatibility of cardiac extracellular matrix (cECM) scaffolds created by decellularization. In this study, porcine hearts were decellularized in an automated perfusion apparatus utilizing sodium dodecyl sulfate (SDS) detergent. Residual SDS was measured by a colorimetric assay. Phosphate-buffered saline, distilled water (DW), and Triton X-100 washes were used to remove SDS. The efficiency of detergent removal was measured as a function of time. It was observed that using Triton-X 100 can nearly double the rate of SDS removal. An assay based on human blood hemolysis was developed to measure the remaining cytotoxicity of the cECM. The results from the hemolysis cytotoxicity assay were consistent with a standard live/dead assay using MS1 endothelial cells incubated with the cECM. This study demonstrated an effective, reliable, and relatively inexpensive method for determining the cytotoxicity and blood compatibility of decellularized cECM scaffolds.

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Section: Discussionmentioning

confidence: 99%

Using Hemolysis as a Novel Method for Assessment of Cytotoxicity and Blood Compatibility of Decellularized Heart Tissues

et al. 2016

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“…Another intriguing analogy can be found with vertebrate cardiac muscle cells that represent an important target of regeneration medicine (Taylor et al, 2014; Kochegarov and Lemanski, 2016; Karra and Poss, 2017; Sommese et al, 2017). In these cells, Ca 2+ currents are also at the base of spike generation, while in skeletal muscle cells spikes rely upon occurrence and propagation of Na + spikes (see Figures 1C,D).…”

Section: Cephalopods Neuro-muscular Systemmentioning

confidence: 98%

Molecular Determinants of Cephalopod Muscles and Their Implication in Muscle Regeneration

Zullo

Fossati

Imperadore³

et al. 2017

Front. Cell Dev. Biol.

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The ability to regenerate whole-body structures has been studied for many decades and is of particular interest for stem cell research due to its therapeutic potential. Several vertebrate and invertebrate species have been used as model systems to study pathways involved in regeneration in the past. Among invertebrates, cephalopods are considered as highly evolved organisms, which exhibit elaborate behavioral characteristics when compared to other mollusks including active predation, extraordinary manipulation, and learning abilities. These are enabled by a complex nervous system and a number of adaptations of their body plan, which were acquired over evolutionary time. Some of these novel features show similarities to structures present in vertebrates and seem to have evolved through a convergent evolutionary process. Octopus vulgaris (the common octopus) is a representative of modern cephalopods and is characterized by a sophisticated motor and sensory system as well as highly developed cognitive capabilities. Due to its phylogenetic position and its high regenerative power the octopus has become of increasing interest for studies on regenerative processes. In this paper we provide an overview over the current knowledge of cephalopod muscle types and structures and present a possible link between these characteristics and their high regenerative potential. This may help identify conserved molecular pathways underlying regeneration in invertebrate and vertebrate animal species as well as discover new leads for targeted tissue treatments in humans.

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“…2 With the currently available therapeutic options, very few patients regain full cardiac function with no long-term side effects following cardiovascular damage. 3 Novel tissue engineering based treatments promise to overcome these limitations, by replacing injured cardiac tissue with artificially created fully functioning muscle and/or inducing endogenous cardiac regeneration. 3 A key part of tissue engineered products are the biomaterial scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Hernández‐Córdova

Mathew

Balint

et al. 2016

J. Biomed. Mater. Res.

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Biomaterial scaffolds are a key part of cardiac tissue engineering therapies. The group has recently synthesized a novel polycaprolactone based polyurethane‐urea copolymer that showed improved mechanical properties compared with its previously published counterparts. The aim of this study was to explore whether indirect three‐dimensional (3D) printing could provide a means to fabricate this novel, biodegradable polymer into a scaffold suitable for cardiac tissue engineering. Indirect 3D printing was carried out through printing water dissolvable poly(vinyl alcohol) porogens in three different sizes based on a wood‐stack model, into which a polyurethane‐urea solution was pressure injected. The porogens were removed, leading to soft polyurethane‐urea scaffolds with regular tubular pores. The scaffolds were characterized for their compressive and tensile mechanical behavior; and their degradation was monitored for 12 months under simulated physiological conditions. Their compatibility with cardiac myocytes and performance in novel cardiac engineering‐related techniques, such as aggregate seeding and bi‐directional perfusion, was also assessed. The scaffolds were found to have mechanical properties similar to cardiac tissue, and good biocompatibility with cardiac myocytes. Furthermore, the incorporated cells preserved their phenotype with no signs of de‐differentiation. The constructs worked well in perfusion experiments, showing enhanced seeding efficiency. © 2016 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1912–1921, 2016.

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Building New Hearts: A Review of Trends in Cardiac Tissue Engineering

Cited by 51 publications

References 51 publications

Using Hemolysis as a Novel Method for Assessment of Cytotoxicity and Blood Compatibility of Decellularized Heart Tissues

Using Hemolysis as a Novel Method for Assessment of Cytotoxicity and Blood Compatibility of Decellularized Heart Tissues

Molecular Determinants of Cephalopod Muscles and Their Implication in Muscle Regeneration

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

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