Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc

Smith-Bolton, Rachel K.; Worley, Melanie I.; Kanda, Hiroshi; Hariharan, Iswar K.

doi:10.1016/j.devcel.2009.04.015

Cited by 261 publications

(521 citation statements)

References 72 publications

Supporting

Mentioning

492

Contrasting

Unclassified

Order By: Relevance

“…2,4,5 One important finding was that the secretion of Wg and Dpp is not limited to undead cells, but also occurs in 'genuine' apoptotic cells. 1,3,4,6 These observations suggested that the proliferation mediated by apoptotic cells might have an important role in CP, 1,3,4 as it would be instrumental in the restoration of normal size after massive cell death. However, flies carrying mutations in both wg and dpp are still capable of CP after high levels of X-rays induced apoptosis.…”

mentioning

confidence: 94%

Compensatory proliferation and apoptosis-induced proliferation: a need for clarification

et al. 2012

View full text Add to dashboard Cite

mentioning

confidence: 94%

Compensatory proliferation and apoptosis-induced proliferation: a need for clarification

et al. 2012

View full text Add to dashboard Cite

“…4, also see ref. 53), is a latent characteristic of lgl − clones that appears to be hastened by down-regulation of N-Wg signaling, or possibly by a concomitant gain of Ras/EGFR signaling. In the eye primordium, switch-to-a-primitive-cell-state is triggered by recruitment of Hth (Fig.…”

Section: -S)mentioning

confidence: 99%

Epithelial neoplasia in Drosophila entails switch to primitive cell states

Khan

Bajpai

Alam

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Only select cell types in an organ display neoplasia when targeted oncogenically. How developmental lineage hierarchies of these cells prefigure their neoplastic propensities is not yet well-understood. Here we show that neoplastic Drosophila epithelial cells reverse their developmental commitments and switch to primitive cell states. In a context of alleviated tissue surveillance, for example, loss of Lethal giant larvae (Lgl) tumor suppressor in the wing primordium induced epithelial neoplasia in its Homothorax (Hth)-expressing proximal domain. Transcriptional profile of proximally transformed mosaic wing epithelium and functional tests revealed tumor cooperation by multiple signaling pathways. In contrast, lgl − clones in the Vestigial (Vg)-expressing distal wing epithelium were eliminated by cell death. Distal lgl − clones, however, could transform when both tissue surveillance and cell death were compromised genetically and, alternatively, when the transcription cofactor of Hippo signaling pathway, Yorkie (Yki), was activated, or when Ras/EGFR signaling was up-regulated. Furthermore, transforming distal lgl − clones displayed loss of Vg, suggesting reversal of their terminal cell fate commitment. In contrast, reinforcing a distal (wing) cell fate commitment in lgl − clones by gaining Vg arrested their neoplasia and induced cell death. We also show that neoplasia in both distal and proximal lgl − clones could progress in the absence of Hth, revealing Hth-independent wing epithelial neoplasia. Likewise, neoplasia in the eye primordium resulted in loss of Elav, a retinal cell marker; these, however, switched to an Hth-dependent primitive cell state. These results suggest a general characteristic of "cells-of-origin" in epithelial cancers, namely their propensity for switch to primitive cell states.

show abstract

“…The imaginal discs that develop into adult appendages can recover from damages caused by physical injury or apoptosis through regenerative growth (McEwen and Peifer, 2005; Smith- Bolton et al, 2009;Wang et al, 2009). The expression of the Wingless (Wg) morphogen in surviving cells is required for this regenerative repair (Ryoo et al, 2004;Smith-Bolton et al, 2009).…”

Section: Knockdown Of Smt3 Leads To Apoptosis and Activates Wg Expresmentioning

confidence: 99%

“…The expression of the Wingless (Wg) morphogen in surviving cells is required for this regenerative repair (Ryoo et al, 2004;Smith-Bolton et al, 2009). We sought to determine whether the Smt3 depletion-induced apoptosis may trigger regenerative growth by examining wg expression.…”

Section: Knockdown Of Smt3 Leads To Apoptosis and Activates Wg Expresmentioning

confidence: 99%

Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus

Huang

Chen

et al. 2011

Development

View full text Add to dashboard Cite

SUMMARYPost-translational modification by the small ubiquitin-related modifier (SUMO) is important for a variety of cellular and developmental processes. However, the precise mechanism(s) that connects sumoylation to specific developmental signaling pathways remains relatively less clear. Here, we show that Smt3 knockdown in Drosophila wing discs causes phenotypes resembling JNK gain of function, including ectopic apoptosis and apoptosis-induced compensatory growth. Smt3 depletion leads to an increased expression of JNK target genes Mmp1 and puckered. We show that, although knockdown of the homeodomaininteracting protein kinase (Hipk) suppresses Smt3 depletion-induced activation of JNK, Hipk overexpression synergistically enhances this type of JNK activation. We further demonstrate that Hipk is sumolylated in vivo, and its nuclear localization is dependent on the sumoylation pathway. Our results thus establish a mechanistic connection between the sumoylation pathway and the JNK pathway through the action of Hipk. We propose that the sumoylation-controlled balance between cytoplasmic and nuclear Hipk plays a crucial role in regulating JNK signaling.

show abstract

Regenerative Growth in Drosophila Imaginal Discs Is Regulated by Wingless and Myc

Cited by 261 publications

References 72 publications

Compensatory proliferation and apoptosis-induced proliferation: a need for clarification

Compensatory proliferation and apoptosis-induced proliferation: a need for clarification

Epithelial neoplasia in Drosophila entails switch to primitive cell states

Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus

Contact Info

Product

Resources

About