Regenerative activity of <i>Hericium erinaceus</i> on axonal injury model using <i>in vitro</i> laser microdissection technique

Üstün, Ramazan; Ayhan, Peray

doi:10.1080/01616412.2018.1556494

Cited by 7 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supplementation of NGF and BDNF have been used to promote survival and axonal regrowth of injured neurons after spinal cord injury (Harvey et al, 2015). In this context, in vitro studies using total extract of H. erinaceus reported regenerative capabilities on peripheral neurons after laser microdissection (Ustun & Ayhan, 2019). This opens the door to additional studies using specific and more potent derivatives from H. erinaceus to identify their potential therapeutical action during axon regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Our study reveals that hericene A enhances recognition memory at concentrations as low as 5 mg/kg/day. The medicinal mushroom H. erinaceus has long been known for its neurotrophic activity on the peripheral nervous system through promotion of NGF synthesis (Haure‐Mirande et al, 2017; Lai et al, 2013; Raman et al, 2015; Ustun & Ayhan, 2019). Several compounds extracted from H. erinaceus have been shown to protect from aging‐dependent cognitive decline in wild‐type (Brandalise et al, 2017; Rossi et al, 2018) and in Alzheimer's disease mouse models (Mori et al, 2011; Tsai‐Teng et al, 2016) suggesting a yet to be defined activity on the CNS.…”

Section: Discussionmentioning

confidence: 99%

“…Compounds derived from H. erinaceus extracts have been shown to promote NGF synthesis and secretion (Kawagishi et al, 1996; Lai et al, 2013; Mori et al, 2009; Raman et al, 2015; Zhang et al, 2015) and to act via the TrkA pathway involving MEK/ERK and PI3K/Akt activation (Haure‐Mirande et al, 2017; Phan et al, 2014). However, the NGF/TrkA‐mediated neuroprotective effects predominantly focus on neurons in the peripheral nervous system (Ustun & Ayhan, 2019). More recently, H. erinaceus extract has been suggested to also affect the BDNF pathway in 1321 N1 human astrocytoma cell line (Rupcic et al, 2018), in the mouse brain (Chiu et al, 2018) and to promote increasing circulating pro‐BDNF levels in humans (Vigna et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hericerin derivatives activates a pan‐neurotrophic pathway in central hippocampal neurons converging to ERK1/2 signaling enhancing spatial memory

Martínez-Mármol

Chai

Conroy

et al. 2023

Journal of Neurochemistry

View full text Add to dashboard Cite

The traditional medicinal mushroom Hericium erinaceus is known for enhancing peripheral nerve regeneration through targeting nerve growth factor (NGF) neurotrophic activity. Here, we purified and identified biologically new active compounds from H. erinaceus, based on their ability to promote neurite outgrowth in hippocampal neurons. N‐de phenylethyl isohericerin (NDPIH), an isoindoline compound from this mushroom, together with its hydrophobic derivative hericene A, were highly potent in promoting extensive axon outgrowth and neurite branching in cultured hippocampal neurons even in the absence of serum, demonstrating potent neurotrophic activity. Pharmacological inhibition of tropomyosin receptor kinase B (TrkB) by ANA‐12 only partly prevented the NDPIH‐induced neurotrophic activity, suggesting a potential link with BDNF signaling. However, we found that NDPIH activated ERK1/2 signaling in the absence of TrkB in HEK‐293T cells, an effect that was not sensitive to ANA‐12 in the presence of TrkB. Our results demonstrate that NDPIH acts via a complementary neurotrophic pathway independent of TrkB with converging downstream ERK1/2 activation. Mice fed with H. erinaceus crude extract and hericene A also exhibited increased neurotrophin expression and downstream signaling, resulting in significantly enhanced hippocampal memory. Hericene A therefore acts through a novel pan‐neurotrophic signaling pathway, leading to improved cognitive performance.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hericerin derivatives activates a pan‐neurotrophic pathway in central hippocampal neurons converging to ERK1/2 signaling enhancing spatial memory

Martínez-Mármol

Chai

Conroy

et al. 2023

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…The authors also concluded that inhibition of the PI3K/Akt pathway through LY294002 reverses the neuroprotective effects of FGF10 in both in vivo and in vitro conditions (Dong et al 2019). Likewise, Üstün and Ayhan 2019 demonstrated the neuroprotective effect of NGF administration in neuronal cultures obtained from dorsal root ganglia (DRG) of 6-8-week aged mice (Üstün and Ayhan 2019).…”

Section: Axonal Regenerationmentioning

confidence: 97%

Free radical biology in neurological manifestations: mechanisms to therapeutics interventions

Tripathi

Gupta

Sahu

et al. 2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

Recent advancements and growing attention about free radicals (ROS) and redox signaling enable the scientific fraternity to consider their involvement in the pathophysiology of inflammatory diseases, metabolic disorders, and neurological defects. Free radicals increase the concentration of reactive oxygen and nitrogen species in the biological system through different endogenous sources and thus increased the overall oxidative stress. An increase in oxidative stress causes cell death through different signaling mechanisms such as mitochondrial impairment, cell-cycle arrest, DNA damage response, inflammation, negative regulation of protein, and lipid peroxidation. Thus, an appropriate balance between free radicals and antioxidants becomes crucial to maintain physiological function. Since the 1brain requires high oxygen for its functioning, it is highly vulnerable to free radical generation and enhanced ROS in the brain adversely affects axonal regeneration and synaptic plasticity, which results in neuronal cell death. In addition, increased ROS in the brain alters various signaling pathways such as apoptosis, autophagy, inflammation and microglial activation, DNA damage response, and cell-cycle arrest, leading to memory and learning defects. Mounting evidence suggests the potential involvement of micro-RNAs, circular-RNAs, natural and dietary compounds, synthetic inhibitors, and heat-shock proteins as therapeutic agents to combat neurological diseases. Herein, we explain the mechanism of free radical generation and its role in mitochondrial, protein, and lipid peroxidation biology. Further, we discuss the negative role of free radicals in synaptic plasticity and axonal regeneration through the modulation of various signaling molecules and also in the involvement of free radicals in various neurological diseases and their potential therapeutic approaches.

show abstract

“…Moreover, an in vivo study conducted using a rat model of global ischemic stroke further revealed the H. erinaceus defensive function, showing the inhibition of inducible NO synthase (iNOS), phosphorylation of p38 MAPK and CHOP, and the reduction of acute inflammatory cytokine levels [46]. Lately, the beneficial regenerative effect of H. erinaceus has been described in a mouse peripheral nerve injury model, demonstrating that, compared to NGF, H. erinaceus possesses higher neuroprotective and neurotogenic action, improving axonal regeneration ability [80]. Additionally, an aqueous extract of H. erinaceus was demonstrated to stimulate neurite outgrowth in NG108-15, a neuroblastoma-glioma cell line, with a synergistic interaction with exogenous NGF [81].…”

Section: H Erinaceus In Vitro Preclinical Studies and Molecular Mecha...mentioning

confidence: 99%

Hericium erinaceus in Neurodegenerative Diseases: From Bench to Bedside and Beyond, How Far from the Shoreline?

Brandalise

Roda

Ratto

et al. 2023

JoF

View full text Add to dashboard Cite

A growing number of studies is focusing on the pharmacology and feasibility of bioactive compounds as a novel valuable approach to target a variety of human diseases related to neurological degeneration. Among the group of the so-called medicinal mushrooms (MMs), Hericium erinaceus has become one of the most promising candidates. In fact, some of the bioactive compounds extracted from H. erinaceus have been shown to recover, or at least ameliorate, a wide range of pathological brain conditions such as Alzheimer’s disease, depression, Parkinson’s disease, and spinal cord injury. In a large body of in vitro and in vivo preclinical studies on the central nervous system (CNS), the effects of erinacines have been correlated with a significant increase in the production of neurotrophic factors. Despite the promising outcome of preclinical investigations, only a limited number of clinical trials have been carried out so far in different neurological conditions. In this survey, we summarized the current state of knowledge on H. erinaceus dietary supplementation and its therapeutic potential in clinical settings. The bulk collected evidence underlies the urgent need to carry out further/wider clinical trials to prove the safety and efficacy of H. erinaceus supplementation, offering significant neuroprotective applications in brain pathologies.

show abstract

Regenerative activity of Hericium erinaceus on axonal injury model using in vitro laser microdissection technique

Cited by 7 publications

References 52 publications

Hericerin derivatives activates a pan‐neurotrophic pathway in central hippocampal neurons converging to ERK1/2 signaling enhancing spatial memory

Hericerin derivatives activates a pan‐neurotrophic pathway in central hippocampal neurons converging to ERK1/2 signaling enhancing spatial memory

Free radical biology in neurological manifestations: mechanisms to therapeutics interventions

Hericium erinaceus in Neurodegenerative Diseases: From Bench to Bedside and Beyond, How Far from the Shoreline?

Contact Info

Product

Resources

About