Regeneration of Uterine Horn Using Porcine Small Intestinal Submucosa Grafts in Rabbits

Taveau, Jon W; Tartaglia, Michele; Buchannan, Dana; Smith, Bret N.; Koenig, G; Thomfohrde, Kevin; Stouch, Bruce C.; Jeck, Saul; Greene, Charlotte H.

doi:10.1080/08941930490422456

Cited by 30 publications

(21 citation statements)

References 61 publications

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“…No obstruction was observed at the implant/native tissue anastomoses as opposed to a study by Jonkman et al [32] in which the artery were occluded by blood clot due to failure of vascularization into scaffold. Patency of the reconstructed uterus was similar to native tissue proving decellularized tissue to be superior to porcine SIS graft [33] where samples larger than 1 mm were found to be twisted due to lack of mechanical strength. Thickness and DNA content of the SDS and HHP group prior to (Fig.…”

Section: Discussionmentioning

confidence: 74%

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

et al. 2014

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Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss and malformations that require uterine reconstruction for the treatment of their infertility. In this study, we are the first to report the usefulness of decellularized matrices as a scaffold for uterine reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining and quantitative analysis showed that both SDS and HHP methods effectively removed cells from the tissues with, specifically, a significant reduction of DNA contents for HHP constructs. HHP constructs highly retained the collagen content, the main component of extracellular matrices in uterine tissue, compared to SDS constructs and had similar content levels of collagen to the native tissue. The mechanical strength of the HHP constructs was similar to that of the native tissue, while that of the SDS constructs was significantly elevated. Transmission electron microscopy (TEM) revealed no apparent denaturation of collagen fibers in the HHP constructs compared to the SDS constructs. Transplantation of the decellularized tissues into rat uteri revealed the successful regeneration of the uterine tissues with a 3-layer structure 30 days after the transplantation. Moreover, a lot of epithelial gland tissue and Ki67 positive cells were detected. Immunohistochemical analyses showed that the regenerated tissues have a normal response to ovarian hormone for pregnancy. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.

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Section: Discussionmentioning

confidence: 74%

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

et al. 2014

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“…In addition, PTFE is likely to cause infection or obstruction due to its nonbioabsorbability, 6 whereas our polymer seems to have a lower risk, because it is absorbed into the body and replaced by native cells. Recently, the small intestinal submucosa (SIS) of pigs has been used for the reconstruction of the artery, 13 urinary tract, 14 and other tissues. Compared with our polymer, SIS, which is derived from the tissue of a nonhuman animal, carries a risk of causing infection with unknown viruses 15 and has been reported to result in tissue shrinkage when used for the reconstruction of the urinary tract and other sites.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Engineered Patch for the Reconstruction of Inferior Vena Cava During Living-Donor Liver Transplantation

Toshimitsu

Miyazawa

Torii

et al. 2005

Journal of Gastrointestinal Surgery

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“…Previously, several scaffolds such as biodegradable microporous synthetic materials, small intestine submucosa, and collagen loaded with collagen-binding human basic fibroblast growth factor8, 9, 10 were used as potential therapies in animal models of AS. However, all the available treatments are not consistently successful and have not been used in humans.…”

Section: Introductionmentioning

confidence: 99%

CXCL12 Promotes Stem Cell Recruitment and Uterine Repair after Injury in Asherman’s Syndrome

Ersoy

Zolbin

Çoşar

et al. 2017

Molecular Therapy - Methods & Clinical Development

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Asherman’s syndrome is an acquired condition of uterine fibrosis and adhesions in response to injury that adversely affects fertility and pregnancy. We have previously demonstrated that bone marrow-derived mesenchymal stem cells (BMDSCs) contribute to uterine repair after injury and that stem cells supplementation improves fertility. Here, we demonstrate that CXCL12 is the chemokine that mediates stem cell engraftment and functional improvement using a murine model of Asherman’s syndrome. After uterine injury, we demonstrate that CXCL12 augmentation increased BMDSC engraftment and that the CXCL12 receptor (CXCR4) antagonist, ADM3100, blocked stem cell recruitment. CXCL12 reduced, whereas ADM3100 increased fibrosis. CXCL12 treatment led to improved fertility and litter size, whereas ADM3100 treatment reduced fertility and litter size. ADM3100 prevented optimal spontaneous uterine repair mediated by endogenous CXCL12 production, reducing pregnancies after injury in the absence of supplemental CXCL12 administration; however, ADM3100 treatment could be partially rescued by CXCL12 augmentation. CXCL12 or other CXCR4 receptor agonists may be useful in the treatment of infertility or adverse pregnancy outcomes in Asherman’s syndrome and other related uterine disorders.

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Regeneration of Uterine Horn Using Porcine Small Intestinal Submucosa Grafts in Rabbits

Cited by 30 publications

References 61 publications

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models

Tissue-Engineered Patch for the Reconstruction of Inferior Vena Cava During Living-Donor Liver Transplantation

CXCL12 Promotes Stem Cell Recruitment and Uterine Repair after Injury in Asherman’s Syndrome

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