Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level

Narmada, Ida Bagus; Laksono, Valerian; Nugraha, Alexander Patera; Ernawati, Diah Savitri; Winias, Saka; Prahasanti, Chiquita; Dinaryanti, Aristika; Susilowati, Helen; Hendrianto, Eryk; Ihsan, Igo Syaiful; Rantam, Fedik Abdul

doi:10.4034/pboci.2019.191.144

Cited by 13 publications

(16 citation statements)

References 25 publications

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“…The rat with hyperglycemic condition confirmed with the blood sugar levels ≥ 250 mg / dL. Previous studies also support our study that mentioned STZ could be used to induce hyperglycemic conditions in rats [5,7].…”

Section: Discussionsupporting

confidence: 89%

“…The 32 samples were divided into 2 groups (n=16): normoglycemic rats (N) (normal blood glucose 80-120 mg/dl) and hyperglycemic rats (H) (>250 mg/dl) induced with Streptozotocin (Sigma Aldrich Inc., St. Louis, MO, USA) at the dose of 30 mg in PBS injection intraperitoneally. In rats, hyperglycemic condition was confirmed if the blood sugar levels ≥ 250 mg / dL checked using Accucheck (EasyTouch GCU, Bioptik Technology Inc., Taiwan) [5].…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the incidence of type II DM is usually found in patients aged 30 years and over, and the most commonly found are patients with an age range of 50-60 years. The number of people with type II DM covers about 90% of the total number of people with DM [3][4][5]. World Health Organization (WHO) reported that the number of people with DM in Indonesia would increase about 250% from 5 million in 1995 to 12 million in 2025.…”

Section: Introductionmentioning

confidence: 99%

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Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement

Alida

Winoto

Narmada

2020

Pesqui. Bras. Odontopediatria Clín. Integr.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement

Alida

Winoto

Narmada

2020

Pesqui. Bras. Odontopediatria Clín. Integr.

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“…28 The other sources of MSCs, such as those derived from hair follicles, the umbilical cord, adipose tissue, and dental pulp, also positively presented the specific cell surface markers of MSCs. 5,6,8,9,11 GDMSCs have a potential proliferation rate characterized by Oct-4, Nanog, and SOX2 expression that play an important role in the self-renewal and survival of MSCs. 41,42 In addition to the proliferation potential of GDMSCs, their migration capability is also good.…”

Section: Discussionmentioning

confidence: 99%

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

et al. 2020

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Objective This study aims to confirm whether the GDMSCs isolated from rabbit’s (Oryctolagus cuniculus) gingiva are mesenchymal stem cells (MSCs). Materials and Methods This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (O. cuniculus) (n = 2), 6 months old, and 3 to 5 kg of body weight. The specific cell surface markers of MSCs; clusters of differentiation (CD), namely, CD44, CD73, CD90, CD105, and CD200 expressions; and hematopoietic stem cell surface markers CD34 and CD45 were examined using flow cytometry and immunohistochemistry with immunofluorescence. The osteogenic differentiation of isolated GDMSCs was examined using alizarin red staining. Results GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits in vitro with a red–violet and brownish color after alizarin red staining. Conclusion GDMSCs isolated from the rabbits (O. cuniculus) were confirmed as MSCs in vitro documented using immunohistochemistry and flow cytometry. GDMSCs can differentiate into osteogenic lineage in vitro that may be suitable for regenerative dentistry.

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“…10 Bone regeneration can be optimally achieved by the application of mesenchymal stem cells (MSCs). 11 The oral cavity is a unique site with potential resources for MSCs such as gingival mesenchymal stem cells (GMSCs), [12][13][14][15] dental pulp mesenchymal stem cells (DPSCs) 16,17 and stem cells from human exfoliated deciduous teeth (SHED). 18 MSCs demonstrate the potential ability to differentiate into various cell types within the mesenchymal lineage through osteogenic, chondrogenic and adipogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

<p>Exfoliated Human Deciduous Tooth Stem Cells Incorporating Carbonate Apatite Scaffold Enhance BMP-2, BMP-7 and Attenuate MMP-8 Expression During Initial Alveolar Bone Remodeling in Wistar Rats (<em>Rattus norvegicus</em>)</p>

Prahasanti¹,

Nugraha²,

Saskianti³

et al. 2020

CCIDE

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Background: Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known to potentially possess the osteogenic ability. Meanwhile, carbonate apatite scaffold (CAS) can act as a biocompatible scaffold capable of supporting mesenchymal stem cells (MSCs) to proliferate and differentiate optimally. The aim of this study is to investigate the expression of bone morphogenic protein-2 and 7 (BMP2, BMP7) and Matrix Metalloproteinase-8 (MMP-8) after the transplantation of SHED-incorporated CAS during in vivo bone remodeling. Material and Methods: A total of 14 healthy, male, Wistar rats, whose mandible anterior teeth were extracted by means of sterile needle holder clamps, constituted the subjects of this study of alveolar bone defects. Two research groups were created: a control group (CAS) as group I and an experimental group (CAS + SHED) as group II. SHED with a density of 10 6 cells were incorporated into CAS before being transplanted into the experimental group. After 7 days, all the animals were sacrificed and their mandible anterior region extracted. The BMP2, BMP7 and MMP-8 expression were subsequently analyzed by means of immunostaining. An unpaired t-test was conducted to analyze the treatment and control group (p<0.01) data. Results: The expression of BMP-2 and BMP-7 was higher in group II compared to group I. Meanwhile, the level of MMP-8 was lower in group II than group I. There was greater significant increased expression of BMP-2 and BMP-7 expression in Group II compared to Group I. There was significant decreased expression of MMP-8 between group II than group I (p<0.01). Conclusion: SHED-incorporated CAS can enhance BMP-2 and BMP-7 expression while attenuating MMP-8 expression during in vivo alveolar bone remodeling.

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Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level

Cited by 13 publications

References 25 publications

Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement

Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

<p>Exfoliated Human Deciduous Tooth Stem Cells Incorporating Carbonate Apatite Scaffold Enhance BMP-2, BMP-7 and Attenuate MMP-8 Expression During Initial Alveolar Bone Remodeling in Wistar Rats (<em>Rattus norvegicus</em>)</p>

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