Regeneration of Plants from Protoplasts of Musa Species (Banana)

Panis, Bart; Sági, László; Swennen, Rony

doi:10.1007/978-3-662-09366-5_8

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…The density of protoplasts was 10 6 × 800 per µl. Subsequently, they also reported the regeneration of plants from protoplasts (Panis et al, 1994 [122] ).…”

Section: Protoplast Culturementioning

confidence: 99%

Biotechnology of the Banana: A Review of Recent Progress

2000

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A number of biotechnological tools have been developed which could help breeders to evolve new plant types to meet the demand of the food industry in the next century. Available techniques for the transfer of genes could significantly shorten the breeding procedures and overcome some of the agronomic and environmental problems which would otherwise not be possible through conventional methods. In vitro protocols have been standardized to allow commercially viable propagation of desired clones of Musa. An overview of the regeneration of banana by direct and indirect organogenesis, and somatic embryogenesis is presented in this article. In addition, the use of several other biotechnological techniques to enrich the genome of banana, such as selection of somaclonal variants, screening for various useful characteristics, cryopreservation, genetic transformation and molecular genetics are reviewed. In conclusion, the improvement of banana through modern biotechnology should help ensure food security by stabilizing production levels in sustainable cropping systems geared towards meeting domestic and export market demands.

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“…The density of protoplasts was 10 6 × 800 per µl. Subsequently, they also reported the regeneration of plants from protoplasts (Panis et al, 1994 [122] ).…”

Section: Protoplast Culturementioning

confidence: 99%

Biotechnology of the Banana: A Review of Recent Progress

2000

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“…The commonly used regulators were auxin and cytokinin (Zhang et al 1998, Park and Son 1992, Assani et al 2001, Panis et al 1994 but ABA and GA were also used for some species (Deng et al 1988, Ara et al 2000, Hartmann et al 1992, Xiao et al 2007.…”

Section: Plant Regenerationmentioning

confidence: 99%

Isolation of Protoplasts Banana (<i>Musa acuminate</i> Colla) cvs. Dwarf Cavendish and Valery and Research Morphological and Cytogenetic Their Plantlets Regenerated

et al. 2017

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Bananas (Musa spp.) are one of the most important fruits in the world. The mesophyll tissue of leaves excised from in vitro plantlets of banana (Musa spp.) cvs. Dwarf Cavendish and Valery were used as a source of protoplast isolation. Aliquots of purified protoplasts were plated in Murashige and Skoog (MS, 1962) liquid culture medium (F) without growth regulators. Protoplast-derived microcolonies were individually picked up and gently transferred onto regeneration medium P (MS medium with IAA and BAP). The highest protoplast numbers were produced in cv. Valery. Microcolonies were regenerated to plantlets, the longest mean of length shoot and the highest shoot proliferation rate were observed in cv. Dwarf Cavendish and cv. Valery, respectively. Chromosome numbers of the plants regenerated from Musa cv. Valery protoplasts varied from 66% normal (2n=3x= 33), 20% (2n=2x+1= 23) and 14% aneuploids (2n=2x 2= 20) of the cells and 54% normal (2n=3x= 33), 26% (2n=2x+1= 23) and 20% aneuploids (2n=2x 2=20) of cells were in cv. Dwarf Cavendish.

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Biotechnology of banana (Musa spp.): multi-dimensional progress and prospect of in vitro–mediated system

Subrahmanyeswari

Gantait

2022

Appl Microbiol Biotechnol

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Regeneration of Plants from Protoplasts of Musa Species (Banana)

Cited by 4 publications

References 14 publications

Biotechnology of the Banana: A Review of Recent Progress

Biotechnology of the Banana: A Review of Recent Progress

Isolation of Protoplasts Banana (<i>Musa acuminate</i> Colla) cvs. Dwarf Cavendish and Valery and Research Morphological and Cytogenetic Their Plantlets Regenerated

Biotechnology of banana (Musa spp.): multi-dimensional progress and prospect of in vitro–mediated system

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