Regeneration of plants from alginate-encapsulated shoot tips of Withania somnifera (L.) Dunal, a medicinally important plant species

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

“…According to most studies, storage at 4 °C is most suitable for many species. In the case of Dalbergia sissoo storage lasted 4 weeks (Chand and Singh 2004), Quercus robur and Quercus cerris 6 weeks (Tsvetkov and Hausman 2005), Eclipta alba and Withania somnifera 8 weeks (Singh et al 2006(Singh et al , 2010. In this paper synseeds were successfully stored even for 12 months, as in the case of shoot apices of Pistacia vera encapsulated with addition of sucrose in artificial endosperm.…”

Encapsulation of Centaurium Erythraea Rafn – An Efficient Method for Regeneration of Transgenic Plants

“…They remained viable longer than encapsulated nontransformed C. erythraea shoot buds (7% after 14 weeks; unpublished data), and also longer than other plant species such as Morus spp. (8-22% after 2-3 months; Pattnaik et al, 1995), Punica granatum (12-16% after 45 days; Naik and Chand, 2006), Withania somnifera (35% after 2 months; Singh et al, 2006) and Psidium guajava (0% after 45 days; Rai et al, 2008). None of the encapsulated C. erythraea HRs or SBs survived after 14 weeks of storage when only water with 3% sucrose was used as the solvent in sodium alginate beads.…”

“…The synthetic seeds can be stored at low temperature and can be used for clonal propagation, conservation and exchange of in vitro plant material between laboratories (Kikowska and Thiem, 2011). Encapsulation of in-vitroderived vegetative propagules for production of synthetic seeds has been widely studied in different plant species such as Momordica dioica (Thiruvengadam et al, 2012), Salvia officinalis (Grzegorczyk and Wysokińska, 2011), Psidium guajava (Rai et al, 2008), Withania somnifera (Singh et al, 2006), Rhodiola kirilowii (Zych et al, 2005), Hibiscus moscheutos (West et al, 2006) and Ocimum sp. (Mandal et al, 2000).…”

An Update on Phytochemicals Analysis and Medicinal Prospects of Indian Herb Withania somnifera

“…Various parts of the plant like Seedlings, embryos, cotyledon, epicotyl, hypocotyl, petiole, leaves, nodes, internodes, stem, shoot tips and roots have been used in different experiments for callus induction, a d v e n t i t i o u s r o o t i n d u c t i o n , r e g e n e r a t i o n , differentiation, flower inductionetc (Singh 2017). Attempt on standardization of cell culture media for encapsulation of shoot tips of along with optimization of media composition has also been taken (Singh ., 2006). The effects of in vitro conditions on accumulation of withanolides, involving organ and callus culture, cell suspension culture and as well as mediated transformation gave an interesting insight on tissue culture of (Singh 2017).…”