Regeneration of Lachenalia Species from Leaf Explants

Abstract

<jats:p>The physiological stage of donor plants determined to a great extent the morphogenic potential of <jats:italic>Lachersalia</jats:italic> (Jacq.) hybrid leaves, but the optimal stage for various cultivars was different. Contact of the bud-forming adaxial epidermal cells with the medium did not significantly stimulate in vitro bud formation on <jats:italic>Lachenalia</jats:italic> leaf explants, but resulted in the formation of callus from the buds of certain hybrids. Wounding on either the adaxial or the abaxial side of leaves had a stimulating effect on certain hybrids, but others did not respond significantly. A reduction in the length of explants from 10 to 3.3 mm resulted in an increase in the total number of buds formed by a specific amount of explant tissue (width of explant = 15 mm).</jats:p>

In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

“…The genus includes many species with excellent horticultural merit (Duncan, 1988). Lachenalia species can be propagated from seed and bulb cuttings, and some from leaf cuttings (Duncan, 1988) and through tissue culture (Klesser and Nel, 1976;Nel, 1983;Niederwieser and Vcelar, 1990). The effects of explant-tissue age and orientation, sucrose, BA, and NAA on adventitious bud formation from leaf tissue have been investigated (Niederwieser and van Staden, 1992;van Staden and Drewes, 1994;van Rensburg and Vcelar, 1989).…”

Review on Adventitious Bud Formation in Lachenalia

“…Adventitious buds form on leaves (Klesser and Nel, 1976;Nel, 1983;Niederwieser and Vcelar, 1990;Niederwieser and Van Staden, 1990 a & b;Perrignon, 1992;Ault, 1995;Suh, Roh and Lee, 1997) bulb-scales, peduncles (unpublished) and axial meristems (Roodbol and Niederwieser, 1998).…”

“…More than 95% of buds w ere observed to originate from derivatives of primary stomatal mother cells, particularly the guard mother cells. Although buds form on the adaxial and abaxial surface of leaf tissue in vitro and on leaf cuttings, the majority of buds form on the adaxial s urface (Niederwieser and Vcelar, 1990;Ndou, 2000). Bud formation shows a strong tendency to take place along the proximal, transverse end of cultured tissue (Niederwieser and Vcelar, 1990;Niederwieser and Van Staden, 1990b) and leaf cuttings (Perrignon, 1992;Ndou, 2000).…”

“…The most evident effect of the genome is on the regeneration potential. Some genotypes formed as much as 35 buds per leaf explant in vitro whereas others formed a few buds per explant (Niederwieser and Vcelar, 1990;Van Staden, 1990, 1992a). Similar genotypic differences were shown for leaf cuttings (Perrignon, 1992;Ndou, 2000).…”

“…Tissue Age. Tissue age has a pronounced effect on the regeneration potential of leaf tissue in vitro (Niederwieser and Vcelar, 1990;N iederwieser and Van Staden, 1990a;Niederwieser and Van Staden, 1992a) and in vivo (Perrignon, 1992;Ndou, 2000). Young tissue generally has the highest regeneration ability, with a tendency to decrease as the age of the tissue increases (proximal, centre and distal parts of leaves).…”