Lachenalia varieties are propagated vegetatively through adventitious bud formation. In this report, published and unpublished results and observations are reviewed. Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems. In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells. The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme. Tissue age affects both the number of buds formed, as well as the size of bud/bulblet. Young tissue proved to be the best source of tissue in several studies. The physiological stage of the mother plant affected bud formation on bud forming tissue. Once plants enter the post anthesis stage, the regeneration potential decreases markedly. This effect is less evident when plant growth regulators (PGR's) are used in vitro. PGR's stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown. Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases. Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.
The distribution of photosynthetates, originating in leaves of different parts of the shoot of Vi tis vinifera L. cv Caber-net Sauvignon at berry set, pea size, veraison and ripeness stages, was investigated. Specific photosynthetic activity of the "CO,-treated leaves gradually decreased during the season. Photosynthetates were hoarded in the leaves at berry set, but were increasingly diverted to the bunches after that. The apical leaves displayed the Itlghes.t.photosynthesis. The leaves opposite and below the bunches accumulated very little photosynthe-tafes, especially from veraison to ripeness. Redistribution of photosynthetates among the basal, middle and apical leaves was generally very restricted at all stages. Multidirectional distribution from the site of application of 14 CO, occurred at berry set stage, while from pea size to ripeness photosynthetates were mainly translocated basipetally. Highest accumulation in the bunches occurred at veraison, while the basal leaves were primarily used to nourish the bunch.
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