Regeneration of Isolated Bulb Scale Segments of Hyacinth*

Pierik, R.L.M.; Woets, J.

doi:10.17660/actahortic.1971.23.71

Cited by 25 publications

(12 citation statements)

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“…Heide (1965) found an interaction between temperature and auxin and cytokinin levels when culturing begonia leaf segments. This was in contrast to the work of Pierik and Ruibing (1973) who suggested that bulblet production was not significantly affected by duration of storage. This was in contrast to the work of Pierik and Ruibing (1973) who suggested that bulblet production was not significantly affected by duration of storage.…”

Section: Stock Plant Temperature Effectscontrasting

confidence: 99%

Cloning: Plants – Micropropagation/Tissue Culture

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Preece

2014

Encyclopedia of Agriculture and Food Systems

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Section: Stock Plant Temperature Effectscontrasting

confidence: 99%

Cloning: Plants – Micropropagation/Tissue Culture

Read

Preece

2014

Encyclopedia of Agriculture and Food Systems

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“…For other bulbous and non‐bulbous plants, explant effects on growth of the organs have been described. In Hyacinthus (Pierik and Ruibing 1973), Hippeastrum (Langens‐Gerrits, unpublished results) and Lachenalia (Perrignon 1992), larger bulblets regenerate from large explants than from small explants. Also in rose (Marcelis‐Van Acker and Scholten 1995) and Vigna radiata (Gulati and Jaiwal 1992) larger organs were obtained from large explants.…”

Section: Discussionmentioning

confidence: 94%

Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro

Langens-Gerrits¹,

Kuijpers²,

Klerk³

et al. 2003

Physiologia Plantarum

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Bulb size is an important factor determining phase change in Lilium: phase change only occurs in bulblets over a certain threshold weight. After phase change has occurred, bulblets sprout with a stem with many leaves. Juvenile bulblets sprout with only a few leaves. The factors contributing to bulb size were studied during in vitro regeneration of bulblets on scale segments. The larger the explants, the larger the regenerated bulblets. Explant size influenced bulb growth during the complete culture period. Bulb growth was stimulated by a high sucrose concentration. The contribution of the medium and the explant reserves to bulb growth were studied in large and small explants using labelled sucrose. Sucrose was mainly taken up through the cut surfaces. In freshly cut explants, the rate of uptake was correlated with the size of the contact area, but at later stages, when regenerating organs were present, the difference in uptake rate of small and large explants almost disappeared. Small explants had a larger sink activity than large ones. Explants with regenerating organs took up more sucrose than freshly cut explants. Sucrose uptake and bulb growth were rather constant in the later phases and doubled when the sucrose concentration was doubled. Partitioning of label from the sucrose over the various organs, was also rather constant in time: approximately 25% was accumulated in the bulblets, 35–45% in the explant and 30–35% was converted to CO2. About half of the label in the explant was recovered at the proximal side where regeneration takes place. Sucrose, once incorporated in the storage pools in the explant, either remained in the explant or was converted to CO2. Redistribution to the growing bulblets hardly occurred. The percentage of bulb growth that could be attributed to uptake of medium components was constant over the regeneration period: 45–50% for large and 65–75% for small explants.

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“…Pierik & Ruibing (1973) stated that the reaction of the different bulbs show a difference in the need for regeneration and growth even if bulbs of the same size and age are used. Research done by Hanks & Rees (1979) showed that the better regeneration was obtained from the outside twin scales of Narcissus, while Hanks (1986) stated that the intermediate twin scales of Narcissus produced the most plantlets.…”

Section: Discussionmentioning

confidence: 99%

Regeneration of bulblets from twin scales ofCrinum macowanii in vitro

Slabbert¹,

Bruyn²,

Ferreira³

et al. 1993

Plant Cell Tiss Organ Cult

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Tissue culture techniques were applied to study the regeneration and growth of bulblets from bulb scale segments of Crinum macowanii Bak. (bush-or march lily) in vitro. Shoots were induced on twin scales taken from the basal plate region of flowering-size bulbs on Murashige and Skoog (MS)-medium containing 0 -2 0 m g l -~ NAA and BA and a modified MS medium (MMS medium) containing 1.25 mg !-~ ancymidoi (A-RestrU), 0.1 mg i-1 NAA and 0.1 mg 1-~ kinetin (ANK). Large bulblets could only be initiated on the latter. Subsequently the bulblets of 5 mm or more in diameter were trimmed and split in half, and secondary plantlets were regenerated on MMS-medium containing ANK or MS-medium without any growth regulators which in turn grew into bulblets suitable for splitting within 12-16 weeks.

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Regeneration of Isolated Bulb Scale Segments of Hyacinth*

Cited by 25 publications

References 0 publications

Cloning: Plants – Micropropagation/Tissue Culture

Cloning: Plants – Micropropagation/Tissue Culture

Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro

Regeneration of bulblets from twin scales ofCrinum macowanii in vitro

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