Regeneration of Haploid Plantlet through Anther Culture of Chrysanthemum (<i>Dendranthema grandiflorum</i>)

Abstract

To observe the possibility of producing haploid plants of Chrysanthemum, anthers of three Korean cultivars 'Yes Morning', 'Hi-Maya', and pot cultivar 'Peace Pink' were cultured. Callus induction among cultivars differed little, but equally good results were obtained with the basal MS medium supplemented with 1 mg/L of 2,4-D, 2 mg/L of BA, 250 mg/L of casein hydrolysate, 45 g/L of sucrose; solidified by 2.75 g/L gelrite. A pretreatment of anthers in media at 4 °C for 48h enhanced the callus induction. Calli were allowed to differentiate on basal MS medium supplemented with 2 mg/L of BA, 0.1 mg/L of NAA, 30 g/L of sucrose; solidified by 2.75 g/L gelrite. Shoot formation from calli in that media slightly differed among cultivars. Multiple shoots elongated from calli were shifted to basal MS medium supplemented with 0.1 mg/L of NAA, 30 g/L of sucrose; solidified by 3 g/L gelrite for rooting. The plantlets with sufficient roots thus obtained were acclimatized and transferred to the soil. Fifty regenerated plantlets from each cultivar were randomly selected for ploidy observation by chromosome counting and haploid plantlet was detected for the garden cultivar 'Yes morning'.

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

“…A significant ( p < 0.05) rooting response was observed in medium containing 0.1 mg/l NAA, with 13.10 ± 0.873 roots at an average length of 136.35 ± 4.316 mm ( Figure 8 ). The auxin NAA was also shown to be best for rooting, in a number of other studies, in various plants, including S. rebaudiana ( Rafiq et al, 2007 ), Dendranthema grandiflorum ( Khandakar et al, 2014 ), and L. pinaster ( de Souza et al, 2007 ). Similar to our study, Ahmed et al (2007) reported that 0.1 mg/l NAA was the best for rooting of S. rebaudiana , with the highest rooting percentage of 97.66%.…”

“…Similar to our study, Ahmed et al (2007) reported that 0.1 mg/l NAA was the best for rooting of S. rebaudiana , with the highest rooting percentage of 97.66%. Furthermore, a study on D. grandiflorum also reported that the in vitro regenerated shoots developed roots well in 0.1 mg/l NAA ( Khandakar et al, 2014 ). Aspilia africana root numbers and lengths were reduced at high concentrations of NAA, possibly due to auxin inhibition effects, as has been reported for Arabidopsis ( Ivanchenko et al, 2010 ).…”