Regeneration of eggplant (Solanum melongena L.) from root explants

Franklin, Gregory; Sheeba, Caroline J.; Sita, G. Lakshmi

doi:10.1079/ivp2003491

Cited by 46 publications

(46 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, callus induction response may be affected by the specificity of explants used for shoot regeneration; thus, explants from 30-days old seedlings revealed better callus induction frequency and subsequent regeneration compared with explants from in vitro developed older plants. Further, the differences in callus and organ induction may be a result of genotype or cultural conditions (Franklin et al, 2004;Magioli et al, 1998;Mir et al, 2008;Scoccianti et al, 2000;Sharma, Rajam, 1995). In our case, duration of callus proliferation on cultural media was very important for shoot regeneration.…”

Section: Discussionmentioning

confidence: 54%

“…A relatively high frequency of callus induction was observed from both the explants in both cultivars and cotyledon was more responsive than hypocotyl in 'LN' than in 'BB'. Previous reports (Franklin et al, 2004;Sharma, Rajam, 1995) on S. melongena show that the type of explants used as callus source and the developmental stage of the seedlings significantly affect callus-and organogenesis. …”

Section: Resultsmentioning

confidence: 99%

“…Macchia et al (1983) induced callus and root formation from leaf explants of F 1 hybrids in eggplants on MS medium containing indole-3-butyric acid (IBA). Induction of organogenic calluses from cultured roots and subsequent differentiation into shoot buds were observed on MS medium in the presence of TDZ and 6-benzylaminopurine (BAP) (Franklin et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Zayova¹,

Vassilevska-Ivanova²,

Kraptchev³

et al. 2012

JCEA

View full text Add to dashboard Cite

A protocol for indirect shoot organogenesis of Solanum melongena 'Larga Negra' and 'Black Beauty' was established using hypocotyl and cotyledon derived calluses. The maximum morphogenic callus induction was observed from cultured cotyledons of 30-days old seedlings on Murashige and Skoog (MS) medium containing 2.0 mg/l α-naphthalene acetic acid and 0.5 mg/l 6-benzylaminopurine. The highest percentage of shoot regeneration and the highest mean number of shoots/callus were obtained on hormone-free MS medium. In terms of callus induction and subsequent plant regeneration, cotyledon explants were more responsive than hypocotyl explants. Regenerated shoots (2-3 cm) were rooted on MS hormone-free medium or medium containing 0.1 mg/l indole-3-butyric acid. About 90% of regenerated plantlets survived under field conditions after hardening in the glasshouse. Several somaclones exhibiting useful variation would to be proposed as initial plant material for eggplant breeding programs.Keywords: callus tissue, eggplant, explants, growth regulator, plant regeneration, somaclonal variation Abbreviations: BAP (6-benzylaminopurine), IAA (indole acetic acid), IBA (indole-3-butyric acid), Kn (kinetin), NAA (α-naphthalene acetic acid), PGRs (plant growth regulators), TDZ (thidiazuron), 2,4-D (2,4-Dichlorophenoxyacetic acid) РЕЗЮМЕ Разработена е ефективна система за растителна регенерация чрез индиректен органогенезис при два сорта патладжан (Solanum melongena), която представлява източник на нови форми растения, ценни като изходен материал за селекционни програми. Подробно са описани всички етапи и условия на култивиране, които водят до получаването на растителни регенеранти и успешно развитие в полски уславия.Ключови думи: експланти, калусна тъкан, патладжан, растежен регулатор, регенерация на растенията, сомаклонално вариране 446Journal of Central European Agriculture, 2012, 13(3), p.446-457 DOI: 10.5513/JCEA01/13.3.1070 РАЗШИРЕНО РЕЗЮМЕ Разработен е протокол за индиректен органогенезис при Solanum melongena от калусни култури, получени от два вида експланти (хипокотили и котиледони). В изследването са включени два сорта патладжан -Larga Negra и Black Beauty. Индуциране на морфогенетичен калус е наблюдаван при котиледони от 30-дневни кълнове, култивирани върху среда Murashige and Skoog (MS), съдържаща 2.0 mg/l α-нафтилоцетна киселина и 0.5 mg/l 6-бензиламинопурин. Определени са цвета, консистенцията и структурата на калуса. Преобладаващата част от експлантите формират калус на 14-15 ден от култивирането. Най-висок процент на регенерация и среден брой пъпки от калус са получени на МС среда без фитохормони. Установено е, че отговорът на котиледоните към формиране на калуси и последваща растителна регенерация е по-висок от този на хипокотилите. Растенията-регенеранти са вкоренени на МС среда без растежен регулатор или на среда, съдържаща 0.1 mg/l индолилмаслена киселина. Степента на преживяемост на регенерантите при полски условия е около 90 %. Получени са сомаклонални вариации, които биха могли да бъдат използвани като изх...

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Zayova¹,

Vassilevska-Ivanova²,

Kraptchev³

et al. 2012

JCEA

View full text Add to dashboard Cite

show abstract

“…It is important to note that the roots are the principal plant material from which drugs are prepared (Sudha and Seeni 2001) and that root culture is an alternative method of both clonal propagation and germplasm conservation (Bernabé-Antonio et al 2010). Additionally, root explants are advantageous over other explants in terms of their easy manipulation and higher degree of regeneration potential (Franklin et al 2004). In the case of plants with sex chromosomes, developing a method of in vitro micropropagation from this type of explants could offer a unique opportunity to obtain true-to-type plants of the same sex, which is essential in cytogenetic research.…”

Section: Introductionmentioning

confidence: 99%

Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets

Ślesak

Góralski

Kwolek

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Rumex thyrsiflorus Fingerh. is one of the few dioecious plant species that have sex chromosomes. The chromosome constitution of females is 2n = 12A ? XX and 2n = 12A ? XY 1 Y 2 of males. It is a medicinally important plant species and has also been the object of studies on the structure and function of sex chromosomes and sex ratio. An efficient plant regeneration protocol was developed from karyologically stable male roots that had been derived from a long-term liquid culture. The root segments were grown on MS medium supplemented with the following plant growth regulators: 2.4-D, NAA, kinetin, BAP and TDZ. The highest frequency (81.73 %) of adventitious shoot formation (16.27 shoots/explant) was obtained on MS ? 0.5 mg/l TDZ. Regenerated shoots were successfully rooted on MS ? 2 % sucrose ? 0.5 mg/l IBA and acclimated to in vivo conditions. Histological analysis revealed indirect (via callus) adventitious shoot formation. The cells of the morphogenetic callus were surrounded by a fibrillar structure that was similar to the extracellular matrix. Molecular analysis based on genetic sex markers confirmed that all of the root explants were male. The genetic stability of the regenerated plantlets was confirmed using random amplified polymorphic DNA analysis. This is the first report concerning the micropropagation protocol for R. thyrsiflorus Fingerh. from male roots derived from a long-term liquid culture, which offers a unique opportunity to obtain true-to-type plants of the same sex.

show abstract

“…The major type of anthocyanins in purple brinjal is nasunin which is identified as delphinidin-3-(pcoumaroylrutinoside)-5-glucoside [17,18] and the isolated extracts have shown to have a high antioxidant activity [19]. The plant has been successfully regenerated by in vitro culture methods using root [20] and leaf [21] explants and also through somatic embryogenesis [22]. But there have been no attempt to produce pigmented callus from purple brinjal.…”

Section: Introductionmentioning

confidence: 99%

Induction of anthocyanin pigment in callus cultures of Solanum melongena L. in response to plant growth regulators and light

Chaudhary¹,

Mukhopadhyay²

2012

iosrphr

View full text Add to dashboard Cite

Anthocyanins are natural pigments which have various health benefits and are potential candidate for use in the pharmaceutical industries. The fruits and vegetables containing anthocyanins have proved to be beneficial for humans due to their antioxidant activity. For the present study purple brinjal (Solanum melongena L.) is selected as the anthocyanin source. The type of anthocyanins in the peels of this vegetable has been determined as nasunin which is identified as delphinidin-3-(p-coumaroylrutinoside)-5-glucoside. The present study attempts to produce pigmented callus from in vitro grown seedlings of purple brinjal as explants. Various combinations of phytohormones were added to the culture media and the induction of pigmented callus was studied. The effect of light intensity was also considered. The best results were recorded when Naphthalene Acetic Acid (NAA) at 1 mg L -1 was used with Kinetin at 0.25 and 0.1 mg L -1 , at a light intensity of 4000 lux. The total anthocyanin accumulation in the pigmented callus was calculated as 70 µg g -1 . This type of study in S. melongena is being reported for the first time to the best of our knowledge.

show abstract

Regeneration of eggplant (Solanum melongena L.) from root explants

Cited by 46 publications

References 13 publications

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets

Induction of anthocyanin pigment in callus cultures of Solanum melongena L. in response to plant growth regulators and light

Contact Info

Product

Resources

About